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Tissue culture method of Fritillaria unibracteata Hsiao

A dark purple fritillary, tissue culture technology, applied in the field of plant tissue culture, can solve the problems of low seedling rate and slow speed of seed propagation

Inactive Publication Date: 2015-07-22
冯文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seed propagation has a low seedling rate and slow speed, and it takes 5 to 6 years to develop into the size of a commercial bulb

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: take the bulbs of Fritillaria cerevisiae as explants, soak them in 75% ethanol for 15 seconds,

[0019] Rinse 3 times with sterile water, then soak in 10% sodium hypochlorite solution for 20 minutes, rinse 3 times with sterile water and set aside.

[0020] (2) Induction culture: Cut the fritillaria bulbs treated in step (1) into pieces of about 0.5 cm and inoculate them in induction medium for adventitious bud induction. After inoculation, it was placed in the light for 12 hours a day, the light intensity was 1000lx, the culture temperature was 25°C, and the relative air humidity was 75% and the induction rate reached 85.7% after culturing for 30 days. The induction medium is: MS+1.0mg / L NAA+0.1mg / L 6-BA+0.5mg / L AC+15g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0021] (3) Proliferation culture: take the adventitious buds induced in step (2) as materials, remove the aged and poorly growing adventitious buds, and cut the adventitious bu...

Embodiment 2

[0025] (1) Disinfection of explants: take the bulbs of Fritillaria cerevisiae as explants, first soak them in 75% ethanol for 25s,

[0026] Rinse 5 times with sterile water, then soak in 15% sodium hypochlorite solution for 25 minutes, rinse 4 times with sterile water and set aside.

[0027] (2) Induction culture: Cut the fritillaria bulbs treated in step (1) into pieces of about 0.5 cm and inoculate them in induction medium for adventitious bud induction. After inoculation, it was placed in the light for 13 hours a day, the light intensity was 2000lx, the culture temperature was 27°C, and the relative air humidity was 75% and the induction rate reached 93.7% after culturing for 30 days. The induction medium is: MS+3.0mg / L NAA+0.5mg / L 6-BA+0.5mg / L AC+25g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0028] (3) Proliferation culture: take the adventitious buds induced in step (2) as materials, remove the aged and poorly growing adventitious buds, and cut the adventitious bud...

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Abstract

The invention discloses a tissue culture method of Fritillaria unibracteata Hsiao. Fritillaria unibracteata Hsiao is a liliaceous fritillary medicinal plant, and the underground bulb of the Fritillaria unibracteata Hsiao is used as medicine and has the effects of clearing heat and removing stasis, preventing phlegm from forming and stopping coughing, and the like. As wild Fritillaria unibracteata Hsiao mostly grows in high-altitude regions, and the growing period is long, shortage of the resource is caused. As a result, the medicinal Fritillaria unibracteata Hsiao has the problem of short supply in the long term. According to the tissue culture method, the scale of the Fritillaria unibracteata Hsiao is taken as an explant, a Fritillaria unibracteata Hsiao in-vitro regenerated plant is obtained by virtue of the processes such as explant sterilization, adventitious bud induction, multiplication culture, rooting culture, and acclimatization and transplanting, and an effective tissue culture rapid propagation technical system of the Fritillaria unibracteata Hsiao is established; as a result, a large quantity of seedlings can be produced in short time, the multiplication efficiency is improved, the production cost can be reduced, and then the breeding and popularization of the Fritillaria unibracteata Hsiao can be accelerated favorably.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture of Fritillaria fritillaria. Background technique [0002] Dark purple fritillary is a rare and precious Chinese medicinal material unique to my country. It is a herb of the genus Fritillaria in the family Liliaceae, and its underground bulbs are used as medicine. It has the effects of clearing heat and moistening the lungs, reducing phlegm and relieving cough, and is used to treat symptoms such as cold cough, dry cough due to lung heat, dry cough and less phlegm. The fritillary fritillary mainly grows on the alpine and plateau grasslands at an altitude of 2800-4500 meters. The growth cycle is slow and the environmental conditions are very harsh. It is expensive and its wild resources are on the verge of exhaustion. Therefore, the supply of dark purple Fritillaria has been in short supply for a long time. [0003] At ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯文杰
Owner 冯文杰
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