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Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum

A technology of Bacillus megaterium and solid fermentation, applied in the field of microbial preparations, can solve the problems of low capacity, restrict the effective utilization of feed resources, etc., and achieve the effects of wide application, improved feed utilization, and short fermentation time

Active Publication Date: 2013-12-25
江苏省苏微微生物研究有限公司 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Agricultural and sideline products such as bran, soybean meal, and fungus bran are rich in nutrients such as carbohydrates, proteins, minerals, and vitamins. They are good sources of animal feed, but their high fiber and low capacity limit their use in feed resources. effective use of

Method used

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  • Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum
  • Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum
  • Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum

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Embodiment 1

[0030] Embodiment 1: Determination of enzyme production characteristics of Bacillus megaterium JSSW-JD-2F5

[0031] (1) Strain activation:

[0032] Aseptically opened the freeze-dried preserved strain of strain JSSW-JD-2F5, streaked and inoculated on the slant of LB agar test tube, cultured at 30°C for 40 hours, and then transferred to the slope of LB agar eggplant bottle by streaking and cultured at 30°C for 40 hours. Microscopic examination showed that more than 90% of the bacteria had formed spores, and mature seeds were obtained.

[0033] (2) Determination of enzyme production characteristics

[0034]Use an inoculation needle to take a small amount of bacteria in the above step (1) and place them on the starch hydrolysis medium plate, casein medium plate, cellulose medium plate and lipase medium plate, and repeat the spotting for each medium 3 times , and cultivated at 30°C for 24h. Add a small amount of Lugol's iodine solution dropwise on the cultivated starch hydrolys...

Embodiment 2

[0044] Embodiment 2: the preparation of bacillus megaterium solid fermentation bacterial agent

[0045] (1) Strain activation:

[0046] Aseptically opened the freeze-dried preserved strain of strain JSSW-JD-2F5, streaked and inoculated on the slant of LB agar test tube, cultured at 30°C for 40 hours, then transferred to the slope of LB agar eggplant bottle, and cultured at 30°C for 40 hours. Microscopic examination showed that more than 90% of the bacteria had formed spores, and mature seeds were obtained.

[0047] (2) Preparation of seed bacterial suspension: scrape and wash the mature seeds from the slope with sterile water, put them into a sterilized triangular flask with sterile glass beads inside, shake and disperse the bacterial sludge to make a uniform bacterial suspension ,spare.

[0048] (3) Solid fermentation culture: put the solid fermentation medium into a fermentation bottle at a volume of 10% (w / v), seal it with eight layers of gauze, and sterilize at 121°C for...

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Abstract

The invention discloses bacillus megatherium, a method for preparing a microbial inoculum through solid fermentation of the bacillus megatherium and an application of the microbial inoculum, belonging to the technical field of microbial preparations. The bacillus megatherium JSSW-JD-2F5 has been preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.8045. By adopting solid fermentation, a preparation method of a bacillus megatherium JSSW-JD-2F5 solid fermentation microbial inoculum and the application of the solid fermentation microbial inoculum are disclosed, wherein the solid fermentation microbial inoculum is used as an additive of a livestock, poultry and fishery cultivation feed and is added into the feed according to a mass percentage of 0.01-0.05%. The product of the microbial inoculum contains high-concentration viable bacteria, active enzyme and physiological active matters, and the concentration of the viable bacteria reaches more than 1.0*10<10>CFU / g and can meet requirements of a feeding microbial additive on the viable count; the application of the inoculum not only can improve the breeding environment, but also has an important significance for increasing the feed utilization ratio and promoting the growth of animals, so that the inoculum is widely applied to livestock, poultry and fishery cultivation.

Description

technical field [0001] The invention relates to a method and application of bacillus megaterium and its solid fermentation for preparing bacterial preparations, belonging to the technical field of microbial preparations. Background technique [0002] Bacillus megaterium ( Bacillus megaterium ) belongs to the genus Bacillus, which can rely on a variety of carbon sources for growth, so it is widely distributed. A variety of enzymes secreted by Bacillus megaterium have application value, such as amylase in bread production and penicillin amidase in the synthesis of new artificial antibiotics. In addition, some Bacillus megaterium can produce proteins with antibacterial effects, and the antibacterial proteins can be further purified as agricultural antibiotics, or their coding genes can be cloned for research on transgenic plants that resist fungal diseases. It can be said that Bacillus megaterium is an excellent bacterial species with multiple functions, safe and efficient, n...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/18A23K1/16C12R1/11
Inventor 孙梅匡群陈秋红夏飞高亮施大林张宪中何义进陈林刘俊美
Owner 江苏省苏微微生物研究有限公司
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