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Method for preparing algin oligosacchride by using algin lyase

A technology of alginate lyase and alginate oligosaccharides, which is applied in the direction of fermentation, can solve the problem of not being retrieved, and achieve the effects of simple economy, excellent stability, and high enzyme yield

Inactive Publication Date: 2009-05-06
OCEAN UNIV OF CHINA
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Problems solved by technology

Except for agarase, no reports on alginate lyase produced by Agarivorans were retrieved
There is no report on the preparation of alginate oligosaccharides by using alginate lyase Agarivorans albus YKW-34

Method used

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Embodiment Construction

[0008] The present invention uses the alginate lyase Agarivorans albus YKW-34 to prepare the method for algin oligosaccharide, which is characterized in that the alginate lyase Agarivorans albus YKW-34 enzyme liquid is prepared first, and the alginate is dissolved in water, and the prepared enzyme liquid is added , in a water bath at 20-55°C, decompose for 2-180 hours, heat up to 60-100°C to inactivate, filter through a membrane, concentrate, and freeze-dry to obtain alginate oligosaccharides.

[0009] When preparing alginate lyase Agarivorans albus YKW-34 enzyme solution, take 1000ml seawater, add 50g kelp powder and 10g potassium nitrate, adjust the initial pH value to 7.0 with NaOH aqueous solution, sterilize and cool to room temperature, add 100ml 12h seed-aged Agarivorans albus YKW-34 strain was shaken at 25°C and 110rpm for 48 hours, the culture solution was taken, centrifuged to get the supernatant, 10g of polyetherimide was added to remove the degradation products of po...

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PUM

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Abstract

The invention relates to a method for preparing algin oligosaccharide by using algin lyase Agarivorans albus YKW-34, which is characterized by comprising the following steps: firstly preparing enzyme liquid of the algin lyase Agarivorans albus YKW-34, dissolving algin in water, adding the prepared enzyme liquid into the solution, decomposing the algin for 2 to 180 hours in a water bath at a temperature of 20 to 55 DEG C, raising the temperature to between 60 and 100 DEG C, inactivating the algin, filtering the algin by a film, and condensing, freezing and drying the algin to obtain the algin oligosaccharide. The method has the advantages that a culture medium for preparing the algin lyase has economic and simple compositions; the algin lyase has high yield, unique Na<+> / K<+> ion dependence, superior temperature and pH stability and high specific activity, and can degrade seminose half aldehyde and guluronic acid fragments of the algin at the same time; and the produced algin oligosaccharide with the polymerization degree of 2 to 12 has favorable application prospect.

Description

technical field [0001] The invention relates to a method for preparing algin oligosaccharides by using algin lyase Agari vorans albus YKW-34. Background technique [0002] Alginate is an important structural polysaccharide in the cell wall of brown algae. It is a linear unbranched polysaccharide composed of two monomers, β-D-mannuronic acid and α-L-guluronic acid. Alginate lyase is a kind of enzyme that catalyzes the degradation reaction of alginate. According to its substrate specificity, it can be divided into mannuronate lyase and guluronic acid lyase. Alginate lyase has a wide range of applications. First, it can catalyze the degradation of alginate into alginate oligosaccharides (AOS) with special activity. AOS can significantly improve the germination and growth of plants, has the activity of scavenging free radicals and anticancer, can improve the leukocyte phagocytosis activity of macrophages to improve human immunity, and can effectively promote the expansion of h...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04C12P19/12
Inventor 付晓婷许加超金相武王永昌王经佐高昕李来好杨贤庆林洪薛长湖徐杰
Owner OCEAN UNIV OF CHINA
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