Antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and microbial organic fertilizer thereof
A technology of microbial organic fertilizer and organic fertilizer, applied in the direction of organic fertilizer, organic fertilizer preparation, biological organic part treatment, etc. The ability to reproduce and function, the effect of enhanced growth and stress resistance, and high bioavailability
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[0022] (1) Isolation and identification of strains
[0023] Acquisition of antagonistic strains: the pathogenic strain Rhizoctonia solani was purchased from the Vegetable and Flower Research Institute of Beijing Academy of Agricultural Sciences, and its pathogenicity was confirmed. Antagonism tests were carried out on NA-improved medium, PDA-improved medium, and LB-improved medium by using the confrontation method, and the antagonistic strains were initially screened from the rhizosphere soil of healthy cucumber plants in continuous-cropping cucumber blight-affected fields, and then passed the pot test. The high-efficiency antagonistic strains were re-screened, and the antagonistic strain NJN43 was finally obtained and identified, and the strain was freeze-dried and stored at -20°C. As a result of the plate confrontation test, it was found that the diameter of the inhibitory zone of antagonistic bacteria against pathogenic bacteria was as high as 2.4cm ( figure 1 ).
[0024...
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