Method for quickly detecting 4-methyl iminazole in food

Abstract

The invention discloses a method for detecting the 4-methyl imidazole in food quickly belonging to food additives detection range. The sample containing caramel pigment is extracted in solid phase through solid phase extraction pile (XTR is more than 500mg), after the sample passes through the pile, it is rinsed with organic solvent, the purged liquid is condensed, uses micro sample applicator to spot sample directly on the chromatography material polyamide film, and it is developed in the developing agent with the same proportion, observes it with naked eye after it is color developed with iodine steam, it observes the brown spots with the rf values the same to the standard sample in rf range between 0.5 and 0.7, then considers that the measured material has 4-methyl imidazole. It can detect if the measured sample is natural or is confected manually, especially proper to detect the sample such as sauce, vinegar and cola containing caramel pigment.

Description

technical field [0001] The invention belongs to the detection scope of food additives, and in particular relates to a method for rapidly detecting 4-methylimidazole in food which can be used as a food production factory to detect caramel pigment and food containing caramel pigment. Background technique [0002] Caramel coloring is a food coloring widely used in the world, with an annual consumption of about 200,000 tons worldwide, of which more than 90% are used in food and beverages. About 95% of caramel coloring in the United States is used in beverages; caramel coloring in Asia is mainly used in soy sauce and other condiments. For example, soy sauce is an indispensable traditional condiment in the life of our people. With the improvement of people's living standards, in addition to requiring soy sauce to have certain nutritional components and sensory quality, higher requirements have been put forward for the safety and hygiene of soy sauce. Color is one of the most impo...

AI Technical Summary

Problems solved by technology

Although the qualitative and quantitative analysis methods of caramel pigment have reached a certain level with the progress of analytical instruments in terms of accuracy and precision, the instrumental analysis methods require special analytical instruments and expensive testing costs

The problems in the method of silica gel thin-layer chromatography and paper chromatography for the determination of 4-methylimidazole contained in caramel pigments are high detection limit, low sensitivity, difficult quantification, and it is not suitable for trace amounts contained in foods added with caramel pigments. 4-Methylimidazole for detection

[0005] At present, the 4-methylimidazole brought in by adding caramel pigment in food lacks a special detection method due to the many components in the food and the difficulty of separation and purification.