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A method for distinguishing teliospores of Tilletia dwarfis and teliospores of Tilletia glabris

A technology of wheat dwarf black ears and teliospores, applied in the field of cell biology, can solve problems such as difficult to distinguish, and achieve the effects of saving detection time, simplifying reagents, and simplifying operations

Active Publication Date: 2021-01-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the study of TFL, ordinary optical microscope is generally used to distinguish, the smut caused by T. tritici and T. dwarf are very similar, and it is difficult to distinguish them by general morphological identification methods

Method used

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  • A method for distinguishing teliospores of Tilletia dwarfis and teliospores of Tilletia glabris
  • A method for distinguishing teliospores of Tilletia dwarfis and teliospores of Tilletia glabris
  • A method for distinguishing teliospores of Tilletia dwarfis and teliospores of Tilletia glabris

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Embodiment 1

[0031] The identification method of embodiment 1.TCK teliospore and TFL teliospore

[0032] 1. Dyeing

[0033] Take one grain of T. dwarf Tilletia galls and one grain of T. glabras galls, put them into small centrifuge tubes respectively, soak in water for 30 minutes, remove the seed coat with tweezers, and centrifuge to remove the supernatant. The samples to be tested were stained with 20 μg / ml WGA-AF488 dye for 60 min, then washed with 1×PBS (pH 7.4) for 3 to 5 times, and stored in 1×PBS for observation. All operations were carried out at room temperature, and the stained samples should be kept away from light.

[0034] 2. Microscopic observation

[0035] Put the teliospores of T. tritici dwarfis and T. glabris after dyeing and washing on concave glass slides respectively, cover them with coverslips and place them under a laser confocal microscope (Leica TCS SP5CLSM) for scanning observation. Select the xyz scanning mode, select the excitation light with an excitation wav...

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Abstract

The invention relates to the field of cell biotechnologies, in particular to a method of identifying a Tilletia controversa Kuhn (TCK) teliospore and a Tilletia foetida (Walle.)Lindr. (TFL) teliospore. The method comprises the steps that a to-be-detected sample suspected to be a TCK teliospore or a TFL teliospore is placed in WGA-AF488 dyestuff for dying, washed with PBS and then placed under a confocal laser scanning microscopy (CLSM) for scanning observation; if the muri and cell wall are both dyed into green, the to-be-detected sample is the TCK teliospore; and if only the cell wall is dyedinto green, the to-be-detected sample is the TFL teliospore. Through the method, the dying result is clear, and the TCK teliospore and the TFL teliospore can be distinguished quickly and accurately.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for distinguishing the teliospores of Tilletia dwarfis and the teliospores of Tilletia glabris. Background technique [0002] Tilletia controversa Kühn (TCK) caused by Tilletia controversa Kühn (TCK) is an important quarantine ear disease of wheat (Hoffmann, 1982), and it is a serious and highly contagious disease. , Difficult-to-control diseases are also one of the important objects for the study of invasive alien organisms in my country. Common Bunt ofwheat is a devastating and global wheat smut caused by Tilletia foetida (Walle.) Lindr., TFL. It was classified as a quarantine object (Li Jianqiang et al., 2003). Due to the difference in the infection mode and the physiology of the pathogenic bacteria, especially the fundamental difference in the teliospore germination and infection environment, it was only in the 1930s that the wheat dwarf smut was distinguished ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N15/14
CPCG01N15/1434G01N15/1468G01N15/1475G01N21/6458G01N2015/1445G01N2021/1787
Inventor 高利张建民刘俭俭陈万权刘太国刘博
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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