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Preventing Bacillus subtilis grown inside diseased plant

A technology of Bacillus subtilis and plant endophytic bacteria, applied in the direction of plant growth regulators, botany equipment and methods, bacteria, etc., to achieve the effects of increasing yield, increasing fresh weight, and promoting plant growth

Inactive Publication Date: 2007-12-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been some reports on Bacillus subtilis with disease resistance, most of them are plant rhizosphere microorganisms, and there are few endophytic Bacillus subtilis isolated from plant tissues. The Bacillus subtilis that can colonize the body of crops, has the effect of disease prevention and growth promotion, and can also produce a variety of antibacterial metabolites has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Liquid preparation

[0030] The endophytic Bacillus subtilis (Bacillus subtilis) Jaas ed1 strain was inoculated on the slant of the test tube, the medium was NA, and cultured at a constant temperature of 25° C. for 2-4 days to obtain the test tube species.

[0031] Inoculate the test tube seed into NB medium, culture it overnight at 30°C with constant temperature shaking, then transfer it to NB medium, and culture it after 3-4 days at 30°C constant temperature shaking culture can be the preparation. It can also be transferred to a fermenter where the fermentation medium is corn flour medium for fermentation and production. Using this corn flour medium for fermentation and culture can simultaneously meet the requirements of high bacterial growth, high production of antibacterial active substances and high yield of spores requirements.

[0032] Formula one, common bacteria NA culture medium (weight %)

[0033] 0.3% beef extract, 0.1% yeast extract, 0.5% pepton...

Embodiment 2

[0042] Embodiment 2 Colonization in multiple crops

[0043] After domesticating the endogenous Bacillus subtilis strain Jaas ed1 resistant to rifampicin, a marker strain resistant to Rif 300 μg / ml was obtained, and the inoculation methods of different inoculation methods of stem acupuncture and root injury were used to measure the marker strain in various plants. Colonization situation. The results show that the marker strain can enter eggplant, rice, wheat, rapeseed, cotton, cucumber and other crop plants through different inoculation methods, and can also reproduce and conduct in the plant. However, different inoculation methods had different effects on the colonization effect of the marker strains, and the method of inoculation with stem acupuncture had the best colonization effect.

Embodiment 3

[0044] Embodiment 3 to the growth-promoting effect of eggplant seedling

[0045]Soak eggplant seeds in clean water for 12 hours, accelerate germination under humid conditions at 30°C, and transfer them to pots filled with sterile soil after the eggplant seeds grow small buds. After the eggplant seedlings grow to two or three true leaves, use wounded Root irrigation method, every eggplant seedling pours 20ml Bacillus subtilis bacterial strain Jaas ed1 bacterium liquid (concentration is 10 8 cfu / ml), pour the bacteria solution again three days later, and use clean water as the control. After three weeks, 15 seedlings were randomly selected, and the plant height, root length, plant weight and dry weight were measured. The results showed that the eggplant seedlings treated with the bacterial solution grew better than the control, with thick stems and large and green leaves. The increase rate of plant height reached 91.64%; the increase rate of root length reached 4.28%, the incr...

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PUM

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Abstract

This invention relates to Bacillus subtilis and its application. Bacillus subtilis Jaas ed1 is stored in China General Microbiological Culture Collection Center on Dec. 12, 2005 with a number of CGMCC No.1564. Bacillus subtilis Jaas ed1 can enter crops with a low bacteria solution concentration, and colonize in crops. Bacillus subtilis Jaas ed1 has good effect on preventing plant diseases, especially soil-borne diseases. Besides, Bacillus subtilis Jaas ed1 can promote plant growth and produce various antibacterial metabolites, and has such advantages as low cost, easy operation, no pollution and no harm.

Description

Technical field: [0001] The invention relates to a plant endophytic bacillus subtilis (Bacillus subtilis) and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique: [0002] Plant endophytic bacteria are microorganisms that can inhabit healthy plant tissues and establish a harmonious relationship with plants without causing substantial harm to plants. A part of plant endophytic bacteria can also benefit plants, such as fixing nitrogen, promoting plant growth or inducing plant disease resistance. In fact, there have been reports about the existence of endophytic bacteria in plants without causing plant diseases more than 50 years ago. From early studies to the 1990s, according to incomplete literature statistics, nearly 60 genera of endophytic bacteria have been isolated from nearly 30 plant species, of which about 2 / 3 are Gram-negative bacteria, so people began to Realize that endophytic bacteria are ubiquitou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02C12R1/125
Inventor 林玲乔勇升周益军顾本康
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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