Sudan red 1 immunity-chromatography test paper detection method
A technology of immunochromatography test paper and detection method, which is applied in the field of bioengineering, can solve the problems of narrow application range, expensive equipment, complicated measurement method, etc., and achieve the effect of simple and convenient operation and high sensitivity
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[0034] Below in conjunction with accompanying drawing, the present invention will be further explained:
[0035] (1) Test paper in the form of 1 test line plus 1 reference line
[0036] The detection line is coated with Sudan Red I polyclonal antibody or Sudan Red I detection antigen at a concentration of 0.1-30 μg / mL, with a width of 1 mm; the reference line is coated with an anti-second species at a concentration of 0.1-30 μg / mL One IgG line of animal protein, 1mm wide; the distance between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection line, which can be greater than or equal to A certain concentration of 10 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is in...
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