Method for determination of multiple nutrient compositions and antibiotic titer changes by using near infrared spectroscopy during production process of antibiotics from microbial fermentation

Abstract

The invention provides a method for simultaneous determination of multiple nutrient compositions and antibiotic titer changes by using near infrared spectroscopy during a production process of antibiotics from microbial fermentation. The method comprises the following steps: spectrums of antibiotic (including aureomycin, terramycin, neomycin and other fermentation type antibiotics) fermentation liquor in different fermentation periods are acquired by using the near infrared spectroscopy technology; each acquired sample is detected by a chemical method, and physical and chemical data of multiple nutrient composition contents and antibiotic titer changes are obtained; the acquired near infrared spectrums as well as the physical and chemical data are fitted for establishing a model. After verification, the model is suitable for detecting nutrient composition contents and antibiotic titer changes in antibiotic fermentation liquor in different periods. The method is used for rapidly feeding back and understanding fermentation cases, timely carrying out nutrition supplement and process control, and monitoring antibiotic titer changes; the whole detection process is highly efficient and saving without pollution.

Description

technical field [0001] The invention belongs to the technical field of near-infrared spectroscopy, and in particular relates to a method for simultaneously measuring the contents of various nutrients and changes in antibiotic potency in the process of microbial fermentation to produce antibiotics by using near-infrared spectroscopy. Background technique [0002] Existing methods for detecting changes in the content of various nutrients and antibiotic potency in fermentation broth require different pretreatments and different instruments for detection according to different components. A sugar meter is required, and the detection of amino nitrogen requires the use of formaldehyde for reaction and titration with sodium hydroxide. The detection of antibiotic potency requires filtration, addition of acid, boiling, constant volume, etc., and then measurement with a spectrophotometer, etc. The existing detection methods are cumbersome, consume a lot of manpower, material resources...

AI Technical Summary

Problems solved by technology

The existing detection methods are cumbersome, consume a lot of manpower, material resources and time, and use a lot of chemical reagents, causing certain pollution to the environment

[0003] The patent application with the publication number CN2881620A discloses an automatic analyzer for antibiotic potency, which includes an antibacterial zone measuring device and a peripheral PC for measuring the diameter of the antibacterial plaque, and automatically distinguishes the results and prints a report; it invented the automatic measurement The device of the potency inhibition zone is only changed on the potency measuring device and cannot be used for the detection of other components, so the detection process of the nutritional components of the fermentation broth and the potency of antibiotics cannot be fundamentally simplified

The patent document with the publication number CN102023140A discloses a method for measuring the content of 1-2 propanediol by NIR technology, which uses near-infrared spectroscopy to measure 1-2 propanediol, but the measured substances do not involve changes in the biological fermentation process, and the measured components are less

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