Clinical-grade human mesenchymal stem cell serum-free complete medium

Abstract

The invention relates to a human mesenchymal stem cell culture medium. According to the culture medium, the basal culture medium comprises the following components based on the final concentration: 1-2g / L of human serum albumin, 5-10mg / L of transferring, 2-8mg / L of fibronectin, 1-4mg / L of laminin, 50g / L of Fe(NO3)3.9H2O, 417g / L of FeSO4.7H2O, 1-3mu g / L of estradiol, 2-5mu g / L of testosterone, 1-3mu g / L of progesterone, 39.25-117.74 mu g / L of dexamethasone, 5-10mg / L of insulin, 376.36mg / L of riboflavin, 80.96-242.87mg / L of coenzyme A, 4.41-6.17mg / L of butanediamine, 1-2mg / L of taurine, 0.61-1.85mg / L of aminoethanol, 8.81-26.42mg / L of pyruvic acid, 3.78-7.56mu g / L of sodium selenate, 292.3-584.6mg / L of L-glutamine, 2-8mu g / L of vascular endothelial growth factor, 4-10mu g / L of epidermal growth factor, 4-10mu g / L of basic fibroblast growth factor, 1-5mu g / L of leukaemia inhibitory factor, 1-5mu g / L of insulin-like growth factor-I and 2-8mu g / L of stem cell factor. The culture medium does not contain the animal serum, the potential animal endogenous endotoxin or virus of the animal serum is eliminated, and the culture medium is conveniently applied to clinics.

Description

Technical field [0001] The invention belongs to the technical field of cell culture, and specifically relates to a human mesenchymal stem cell culture medium. Background technique [0002] Stem cells (SC) are a type of self-renewing pluripotent cells. Under certain conditions, they can differentiate into a variety of functional cells, which can be used to treat leukemia, congenital metabolic diseases, certain Various diseases such as solid tumors, diabetes, heart disease and cerebral palsy have very broad medical uses. As of December 2012, 4178 stem cell clinical experimental studies have been carried out globally, and as many as 602 projects have entered clinical phase III. Mesenchymal stem cells (MSC) are currently the safest, most effective and widely used adult stem cells in treatment, mainly derived from bone marrow, fat, umbilical cord, placenta, amniotic membrane and so on. As of April 2013, 7 kinds of stem cell clinical treatment products have been approved for marketing...

AI Technical Summary

Problems solved by technology

There is a risk of potential virus infection in animal-derived serum, and the serum components are complex, it is not easy to qualitatively study the effect of each component on the growth and differentiation of stem cells, and it is easy to cause allergic reactions

[0004] At present, there are many mesenchymal stem cell culture medium products on the market. Disadvantages: 1. The use of animal-derived serum increases pathogens, heterologous antigens, and instability between batches

The US FDA is considering banning the use of fetal bovine serum for cell therapy; 2. Stem cells are easy to differentiate and cannot maintain stemness for a long time

3. Without a full set of third-party pathogen testing (14 tests), a full set of third-party pathogen testing reports cannot be provided. The cultured cells have the risk of pathogen contamination and have not reached the clinical level.

4. Several serum-free culture media for human mesenchymal stem cells can be purchased in the market, but all of them are imported from abroad, and the price is expensive. The price per 500ml is more than 3,000 yuan, but the culture effect is not ideal. The expression of stem cell surface markers is not ideal

And it is for research application only, not for human diagnosis and treatment

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