Wall-breaking method of ganoderma lucidum spore
A technology of Ganoderma lucidum spores and Ganoderma lucidum is applied in the field of breaking the walls of Ganoderma lucidum spores by a biological method, which can solve the problems of high price, complex mechanical equipment structure and high operating cost, and achieves easy operation, elimination of harmful biological residues, and technological progress. The natural effect of the process
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[0036] Referring to Fig. 1, the inventive method comprises the following steps:
[0037] 1. Isolation and purification of endophytic bacteria from Ganoderma lucidum:
[0038] 1) Isolate the endogenous bacteria of Ganoderma lucidum: first wash the surface of fresh Ganoderma lucidum with tap water, soak it in mercuric chloride for 3 minutes, then rinse and sterilize it with tap water, cut it into small pieces of appropriate length and soak it in 75% alcohol for 3~ 5min, rinse with sterile water 3 to 4 times to sterilize again, blot dry with sterile filter paper, then use a sterile blade to cut off the epidermis of the tissue and cut into small pieces of 0.5cm×0.5cm×0.1cm and put them on the beef Put it on the paste peptone medium, and culture it in a 35-37°C incubator for 3-20 days. After hyphae grow around each plant tissue on the medium, pick the edge part and transfer it to a new beef extract peptone plate medium, and continue Place them in a 35-37°C incubator for 3-7 days. ...
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