Method for producing biological pesticide by using endophytic bacillus subtilis

Abstract

The invention relates to a method for producing a biological pesticide by using endophytic bacillus subtilis, and belongs to the field of biological pesticide preparation. According to the method, the strain of the endophytic bacillus subtilis is subjected to shake cultivation for 24 hours in a seed culture solution containing 0.3% of beef extract, 1% of peptone, and 0.5% of NaCl at a temperature of 32 DEG C and a rotation speed of 170 r / min, wherein the resulting seed culture solution is adopted as the production bacteria seed; the resulting seed culture solution is inoculated in a fermentation culture medium comprising soybean cake powder, soluble starch, peptone and NaCl to carry out fermentation culture for 72 hours at a fermentation temperature of 30-34 DEG C under a condition of the pH value of 5-9, wherein the inoculation amount of the seed culture solution is 6% of the fermentation culture medium; the fermentation culture medium with the bacterial concentration more than 0.5*10<9> cfu / mL is subject to spray drying to prepare into the wettable powder with the bacterial content more than 2.0*10<9> cfu / g. With the method of the present invention, the characteristics of the endophytic bacillus subtilis are adopted, such that the significant prevention and treatment effect of the wheat sharp eyespot is provided, wherein the characteristics of the endophytic bacillus subtilis are that: the endophytic bacillus subtilis can colonize on the wheat rhizosphere and in the body, and the endophytic bacteria can produce biological surface active element (surfactin) antibacterial matters.

Description

technical field [0001] The invention relates to a method for producing biological pesticides, in particular to a method for producing biological pesticides with endophytic bacillus subtilis. Background technique [0002] Wheat sheath blight has become a common disease in my country's wheat regions. After wheat is infected by Rhizoctonia solani, symptoms such as rotten buds, dead seedlings, rotten flower stems, and white ears of dead plants appear at various growth stages. The rotten buds and scabs are browned, and the rear buds are dead and rotten, and cannot be unearthed; the dead seedlings occur at the 3-4 leaf stage, and only the first leaf sheath is gray in the middle at the beginning, and there are brown lesions around it. The diseased seedlings die; after the rotten stems are jointed, a moiré-like lesion in the middle gray with light brown edges is formed on the leaf sheath at the base. Behind the stem wall, grayish-brown in the middle and brown near-round or oval ey...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of medium and long-term prevention and control of wheat sheath blight, large-scale application of chemical pesticides is easy to cause environmental pollution, drug resistance and pesticide residues, etc., the present invention provides a method for producing biological pesticides by using endophytic bacillus

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