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Bacillus subtilis BS193 for preventing and controlling potato late blight and application of bacillus subtilis BS193

A technology of Bacillus subtilis and potato late blight, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of unsatisfactory chemical control effects and environmental pollution, and achieve the prevention and control of potato late blight, reduction of agricultural pollution, and simple cultivation conditions Effect

Inactive Publication Date: 2017-08-01
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of biocontrol Bacillus subtilis and its application for efficient prevention and treatment of potato late blight in view of the serious occurrence of potato late blight at present, the area is expanding year by year, the effect of chemical control is not ideal, and the problem of environmental pollution is brought method

Method used

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  • Bacillus subtilis BS193 for preventing and controlling potato late blight and application of bacillus subtilis BS193
  • Bacillus subtilis BS193 for preventing and controlling potato late blight and application of bacillus subtilis BS193
  • Bacillus subtilis BS193 for preventing and controlling potato late blight and application of bacillus subtilis BS193

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment one: the identification of bacterial strain BS193

[0022] 1. Source of the strain

[0023] Strain BS193 was isolated from healthy tomato leaves. After washing the tomato leaves with tap water and drying them, select an appropriate amount of leaf tissue, wash with distilled water three times, disinfect with 75% ethanol for 3 minutes, clear with sterile water for 3 times, treat with mercury chloride for 10-15 minutes, and then wash with sterile water 3 times. Add 10 mL of sterile water and sterilized quartz sand into a sterile mortar, grind it into a homogenous slurry, cover it and let it rest for 30 minutes, suck 50 μL of the supernatant with a pipette gun and spread it on the NA medium, and after 48 hours, Colonies were grown for purification and preservation.

[0024] , observation of morphological characteristics

[0025] Streak inoculate the strain BS193 in LB medium (10 g tryptone, 5 g yeast powder, 5 g sodium chloride and 15 g agar powder, add disti...

Embodiment 2

[0028] Example 2: Antagonistic assay of bacterial strain BS193

[0029] Using the flat plate confrontation culture method, the bacterial strain BS193 was tested for antagonism against P. infestans. First, the mycelium block of P. infestans was inserted in the center of the rye medium plate, and when the colony of P. The strain BS193 was connected to the week point, and the width of the inhibition zone of the strain BS193 against Phytophthora sojae was measured after culturing at 25°C for 15 days. The results of the screening test showed that the strain BS193 had a good antagonistic effect on Phytophthora infestans ( image 3 ).

Embodiment 3

[0030] Embodiment three: bacterial strain BS193 fermented liquid preventive effect test

[0031] BS193 strain fermentation broth (10 8 cfu / mL), centrifuge at 8000r / min for 20min, and collect the cell pellet and supernatant respectively. After the bacterial cell sedimentation is washed several times with sterile water, the bacterial cell suspension with the same volume as the original fermented bacterial liquid is prepared with sterile water. Potatoes were sown in nutrient pots filled with sterile soil, four plants per pot. The roots were irrigated with 20 mL of strain culture liquid, bacterial suspension, and sterile filtrate, and 24 h later inoculated with 10 mL of Phytophthora spore suspension (concentration of sporangia 1×10 4 / mL). Each treatment was repeated 3 times, and inoculated with P. infestans after treatment with clean water and LB medium as the control, and treated with moisture. The disease incidence was investigated 3 days after inoculation.

[0032] The di...

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Abstract

The invention provides bacillus subtilis BS193 for preventing and controlling a potato late blight and application of bacillus subtilis BS193 and belongs to the technical field of crop disease prevention and control. A strain is Bacillus subtilis BS193 and has been recorded and preserved by China General Microbiological Culture Collection Center at March 27, 2017 with a preservation number being CGMCC No. 13936. The strain inhibits growth of germs of the potato late blight by generating active substances and expresses better preventing and controlling effect to the potato late blight. The fungicide can be used for atomizing or root irrigation of a potato plant when being applied and can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer. The bacillus subtilis BS193 provided by the invention is obtained by being extracted from a tomato plant body, belongs to endophyte, is nontoxic, does not have pathogenicity, and thus having a good application prospect in biological prevention and control of the potato late blight.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain bacillus subtilis BS193 for preventing and treating potato late blight and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique [0002] by Phytophthora infestans ( Phytophthora infestans Late blight caused by (Mont) de Bary) is a devastating disease in potato production. It occurs and is prevalent in all major potato producing areas in the world, and seriously threatens potato production. The damage it causes, the difficulty of control and the The social impact has surpassed rice blast and wheat stripe rust, and is regarded as the largest crop disease in the world. Late blight is common and serious in our country, causing an economic loss of about 8 billion yuan each year due to late blight, which has become the first major obstacle to restricting potato production and industrialization in my country. [0003] So far, the preventio...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/125
CPCA01G13/00A01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 陈庆河李本金王荣波刘裴清肖小露翁启勇
Owner INST OF PLANT PROTECTION FAAS
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