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Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds

A technology of Pistacia chinensis and endophytic bacteria, applied in the field of biotechnology and modern agriculture, can solve the problems of unsatisfactory disinfection effect of Pistacia chinensis, and achieve the effect of increasing germination rate and reducing pollution rate

Active Publication Date: 2012-06-20
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The object of the present invention is to aim at the unsatisfactory problem of the general disinfection effect of Pistacia chinensis, and to design a method that not only has a good disinfection effect but also has a high germination rate and robust growth of the aseptic seedlings obtained.

Method used

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Examples

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Embodiment 1

[0012] Example 1: The specific operation method for the removal of endophytic bacteria and rapid germination of Pistacia chinensis seeds is as follows:

[0013] 1. Wash the Pistacia chinensis seeds with the shell removed several times with pure water, then treat them with 70% ethanol for 1 minute, rinse them once with sterile water, and then treat them with 20% sodium hypochlorite for 10 minutes. Rinse with bacterial water 3-4 times;

[0014] 2. Soak the washed seeds in a sterile Erlenmeyer flask, put them into an ultrasonic instrument at 20°C for 10 minutes, and then rinse them with sterile water until they are clean;

[0015] 3. Inoculate the sterilized seeds in the germination medium 1 / 2MS+6-BA2.0 mg / L+SNP15 mg / L+glucose 20g / L+agar 8 g / L, and you can get no endophyte contamination, Sterile seedlings with high germination rate and strong growth.

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Abstract

The invention discloses a method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating the pistacia chinensis bunge seeds. The method mainly comprises the following steps of: sterilizing the pistacia chinensis bunge seeds, of which the shells are removed by using ethanol and sodium hypochlorite; soaking the seeds in water to perform ultrasonic processing; and flushing the seeds, and then inoculating the seeds to a seed germination culture medium to obtain aseptic robust seedlings. By adoption of the method, the difficulty that the germination rate of the seeds is influenced by pollution caused by inexhaustive sterilization and long sterilization time is solved, and more importantly, the germination rate of the seeds is increased, the seedlings grow robustly, and a basis is provided for tissue culture and genetic transformation of pistacia chinensis bunge.

Description

technical field [0001] The invention relates to the fields of biotechnology and modern agricultural technology, in particular to a method for removing endophytic bacteria and rapidly germinating seeds of Pistacia chinensis. Background technique [0002] Pistacia ( pistacia chinensis Bunge) is an important perennial woody oil plant with a wide range of distribution. It can be planted in barren mountains and sandy lands. It is suitable for large-scale afforestation, and the oil content of its seeds is as high as 40%. Biodiesel produced from Pistacia chinensis seeds is clean Renewable energy is a high-quality substitute for petroleum diesel, and has been identified as a priority tree species for future ecological energy forest construction and biodiesel industry. Due to its promising prospects as a biomass energy tree species, there are more and more studies on Pistacia chinensis, but they are basically concentrated on cultivation techniques and other aspects, while the...

Claims

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Application Information

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IPC IPC(8): A01C1/02A01C1/08
Inventor 吴丽芳侯金艳李明浩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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