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Clonostachys rosea, Clonostachys rosea liquid and application of Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary

A technology of Glioma pink and Glioma pink, applied in the fields of application, fungicides, fungi, etc., can solve problems that do not meet the road and direction of green sustainable development, affect the yield and quality of sunflowers, and pesticide residues

Active Publication Date: 2021-05-07
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually these operations are cumbersome and chemical pesticides will cause pesticide residues and other problems, which will affect the improvement of sunflower yield and quality. Chemical control will cause environmental pollution, which is not in line with the road and direction of green and sustainable development.

Method used

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  • Clonostachys rosea, Clonostachys rosea liquid and application of Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary
  • Clonostachys rosea, Clonostachys rosea liquid and application of Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Preparation of Gliocus pink MES72701 bacterial liquid

[0028] This embodiment provides a method for preparing a Gliocladium rosea MES72701 bacterial liquid, which includes the following steps.

[0029] 1. Inoculation and cultivation

[0030] Pick the fresh mycelium of MES72701 that has been activated and cultured, inoculate it on solid PDA medium, inoculate 3 plates, and then place it in a constant temperature incubator at 28°C for 15 days. The formula of PDA medium is: glucose 20g / L, potato 200g / L, agar 19g / L, water 1000mL, natural pH; the configuration method of PDA medium refers to the preparation of conventional potato glucose medium.

[0031] 2. Preparation of spore solution

[0032] After 15 days, 25 mL of sterile water (containing 0.05% Tween 80) was added to each petri dish, the spores were washed with a spreader, filtered with four layers of gauze, and a 10-fold gradient dilution was performed.

[0033] 3. Detection of spore content

[0034] ...

Embodiment 2

[0035] Embodiment 2: Bacteriostasis test of Glioma pink MES72701 bacterium liquid to sunflower sclerotinia

[0036] 1. Test time and place: In December 2019, it will be carried out in the laboratory of the company's R&D center.

[0037] 2. Test method:

[0038] (1) The source of the tested bacteria

[0039] Sclerotinia sclerotiorum was isolated from the sunflower sclerotiorum pathogenic field in Chifeng City, Inner Mongolia, cultivated and purified by the R&D personnel of the company, and finally identified as Sclerotinia sclerotiorum (Lib.) deBary by 16S rDNA, and identified as Sclerotinia sclerotiorum (Lib.) deBary by pathogenicity assay Strong pathogenicity.

[0040] Glioma pink fungus liquid is obtained by inoculating, culturing, and preparing spore liquid from the bacterial species described in Example 1.

[0041] (2) Plate antagonism experiment

[0042] To prepare a PDA solid plate, pipette 200 μL of the spore suspension prepared in Example 1 and apply it evenly on t...

Embodiment 3

[0046] Embodiment 3: Potted control effect of Glioma pink MES72701 bacterium liquid to sunflower sclerotinia

[0047] 1. Test drug

[0048] (1) Microbial agent

[0049] Glioma pink MES72701 bacterium liquid (MES72701 spore suspension) prepared in embodiment 1, spore content is 2 * 10 7 individual / mL.

[0050] (2) Chemical agents

[0051] 50% procymidone wettable powder 2000 times liquid (Shandong Haixun Biochemical Co., Ltd.).

[0052] (3) Blank control

[0053] Set clean water treatment as blank control.

[0054] 2. Test time and place

[0055] The test was carried out in the company's nursery in December 2019.

[0056] 3. Test method

[0057] Treatment settings: the sunflower variety is Longshikui No. 2, and the test set 5 treatments, which are respectively the original concentration of the spore suspension described in Example 1, dilution 1 times, dilution 3 times, dilution 5 times and adding water without adding bacteria control , 50% procymidone wettable powder 2...

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Abstract

The invention relates to Clonostachys rosea, Clonostachys rosea liquid and application of the Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary. The collection unit name of the Clonostachys rosea MES is China General Microbiological Culture Collection Center, and the collection number is CGMCC 3.11519. The Clonostachys rosea liquid contains 1.8 * 10<7> to 2.3 * 10<7> Clonostachys rosea spores per mL. The preparation method comprises the following steps of: picking fresh activated and cultured Clonostachys rosea hyphae, inoculating a plate containing a solid PDA (potato dextrose agar) culture medium with the fresh activated and cultured Clonostachys rosea hyphae, and culturing in a constant-temperature incubator at 25-30 DEG C for 12-16 days; and adding 20-30mL of Tween 80-containing sterile water into the culture dish, washing off the spores with a coater, filtering, and carrying out 8-12-time gradient dilution. The Clonostachys rosea liquid provided by the invention has obvious antagonistic effects on sclerotinia sclerotiorum causing Sclerotinia sclerotiorum(Lib.)deBary, and is capable of preventing or relieving the occurrence of Sclerotinia sclerotiorum(Lib.)deBary, promoting stable yield and income increase of sunflowers and improving the yield and quality of sunflowers.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to Gliocladium pink, Glioma pink fungus liquid and its application in preventing and treating sunflower sclerotinia. Background technique [0002] Sunflower is an important oil crop in my country. In recent years, the sunflower production areas in my country have been severely damaged by Sclerotinia sclerotiorum, and even caused the extinction of sunflower production. It will affect the improvement of sunflower production and quality, and hinder the development of sunflower industry. [0003] Sunflower sclerotinia (Sclerotinia sclerotiorum (Lib.) deBary) is called Sclerotinia sclerotiorum, which belongs to Ascomycota subphylum fungus. The fungus survives the winter in the soil, in diseased debris and between seeds with sclerotia. Pathogenic bacteria can grow in the temperature range of 0-37 ℃, and the optimum temperature for growth is 25 ℃....

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 顾欣燕肖培英赵晓琛李立培刘刚
Owner 天津开发区坤禾生物技术有限公司
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