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Biological prepn. for preventing and controlling soft rot of konjak, and its application

A biological agent and soft rot technology, applied in biocides, chemicals for biological control, applications, etc., can solve problems such as polluting the environment, low feasibility, and affecting the growth of foreign exports

Inactive Publication Date: 2006-06-28
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For konjac bacterial soft rot, agricultural measures such as crop rotation and large doses of chemical pesticides are currently the main control measures in my country. Lower and lower; chemical control not only has high cost and poor control effect, but also pollutes the environment and affects the quality, and some pathogenic bacteria show resistance to the commonly used agricultural streptomycin in production, making the control effect even worse
In addition, due to the use of chemical pesticides and pesticide residues exceeding international standards, the growth of its exports has been affected.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) C 3 Transfer to the test tube slant medium, the formula of the test tube slant medium is: beef extract 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, agar 1.7%, pH: 7.0.

[0082] Incubate at 28°C for 2 days to obtain test tube species.

[0083] The formula of the liquid fermentation medium is: soybean powder 2.0%, starch 1.0%, glucose 0.30%, KH 2 PO 4 0.1%, peptone 0.2% (NH 4 ) 2 SO 4 0.05%, MgSO 4 .7H 2 O 0.03%, CaCO 3 0.1%, peanut oil 0.1%, defoamer 0.01%, pH 7.5;

[0084] The test tube seed is inoculated in 500ml triangular flask (200ml of every bottle) liquid medium, and medium formula is: beef extract 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, agar 1.7. Shake culture at a constant temperature of 28° C. for 14 hours at a rotation speed of 150 rpm, and the obtained culture solution is the biological preparation of the present invention.

Embodiment 2

[0086] At first obtain test tube species (same as embodiment one), test tube species is inoculated in 500ml triangular flask (200ml of every bottle) liquid medium, medium formula is: beef soak 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, agar 2.0%. Shake culture at a constant temperature of 28° C. for 14 hours at a rotational speed of 200 rpm, transfer the obtained culture solution to a fermenter for fermentation production, and obtain a liquid preparation.

[0087] The formula of the liquid fermentation medium is: soybean powder 2.0%, starch 1.0%, glucose 0.30%, KH 2 PO 4 0.1%, peptone 0.2% (NH 4 ) 2 SO 4 0.05%, MgSO 4 .7H 2 O 0.03%, CaCO 3 0.1%, peanut oil 0.1%, defoamer 0.01%, pH 8.0.

[0088] Liquid fermentation conditions

[0089] Tank temperature: the tank temperature of the fermenter is controlled at 25°C, measured by a thermometer inserted into the medium, and adjusted by passing cooling water or hot water into the interlayer.

[0090] Tank pressu...

Embodiment 3

[0097] At first carry out the liquid fermentation culture of bacterium (same as embodiment two). Then, it is made into wettable powder by adding filling agent, filtering with plate and frame, beating, drying and pulverizing. The specific process is as follows:

[0098] Specifically add the filler: put the fermentation hydraulic pressure into the storage tank, add the filler diatomite (light calcium carbonate) according to 10% of the volume of the tank, and stir for 30 minutes after adding.

[0099] Plate and frame filtration: Press the material from the storage tank into the plate and frame with a pressure of 2 kg / cm2, and filter through the No. 7 filter cloth. The pressure should be adjusted at any time so that the bacteria content in the filtrate does not exceed 0.2 billion / ml.

[0100] Beating: unload the filter cake into the beating tank, add thick milk No. 100 at 8% of the amount of calcium carbonate added, or add SDS at 3%, add 2% of the filtrate and stir evenly for 30 ...

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PUM

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Abstract

A biological product in the form of liquid or wettable powder for preventing and eliminating the bacterial soft rot of konjak is prepared from the Bacillus amyloliquefaciens C3 (CGMCC No.1553) through liquid fermenting to obtain the cultured substance, adding wetting agent, adhesive and additive, and preparing liquid or wettable powder.

Description

Technical field: [0001] The invention relates to a biological preparation for preventing and treating bacterial soft rot of konjac and its application, belonging to the technical field of biological pesticides. Background technique: [0002] Konjac is rich in glucomannan. It is one of the few plants containing natural glucomannan in nature, and it is also the only plant with wide distribution, strong adaptability, and large-scale extraction of glucomannan. It is a medical health food and industrial raw material with great development potential. With the continuous development of konjac products and the adjustment of rural industrial structure, konjac has become a major economic crop in the mountainous areas of central and western my country, and it is one of the effective ways for farmers to get rid of poverty and revitalize the local economy. According to incomplete statistics, in 2005, the planting area in Yunnan Province reached 150,000 mu, and the national planting area...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 姬广海李成云叶敏吴亚鹏
Owner YUNNAN AGRICULTURAL UNIVERSITY
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