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A kind of tobacco matrix composite biopesticide and its manufacturing method

A biopesticide and manufacturing method technology, applied in the direction of pesticides, botanical equipment and methods, biocides, etc., can solve the problem of underutilization of resources

Inactive Publication Date: 2011-12-07
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct use of tobacco waste as pesticides, the above resources are not fully utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Select 1000kg of waste tobacco stems produced by threshing and redrying plant, put it in the soaking tank, add 10000kg of water, mix well, soak at room temperature for 2 days, stir appropriately during this period to fully dissolve the solubles; then release the extract, The material is drained and sent to the press for solid-liquid separation. The liquid is combined with the aforementioned extract, 0.5% peptone and 0.3% calcium carbonate are added, and the pH is adjusted to 7.2. After high temperature sterilization, the tobacco stem liquid medium is obtained. spare.

[0050] Domestication and cultivation of Bacillus thuringiensis (Bt) primary slant strains: Take a small amount of the above liquid medium, and insert Bacillus thuringiensis (Bt) slant strains into the tobacco stem liquid medium, and culture it with shaking at 28°C for 24 hours , Then transfer to the same medium for culture, follow this method to transfer 3 to 5 times in succession, dilute the obtained medium...

Embodiment 2

[0055] According to the method in Example 1, the preparation of tobacco stem liquid medium and the expansion culture of Bacillus thuringiensis (Bt) secondary strains were carried out, and then solid-state fermentation was used to produce Bacillus thuringiensis (Bt) insecticide: 1000kg tobacco stem Break the waste to a particle size of 0.2~2cm, add 800kg of water, mix well, adjust the pH to 7.2, sterilize at 121°C for 20 minutes, after cooling, connect to Bacillus thuringiensis (Bt) secondary species (inoculation amount is 30%) , At a temperature of 28 ~ 35 ℃, aeration culture for 3 days to obtain the fermented material, the fermented material is dried and pulverized to obtain a powdered tobacco matrix Bacillus thuringiensis (Bt) compound insecticide, and its bacterial content is determined to be 1.5×10 10 Pieces / g, the total amount of tobacco insecticidal components is 12%.

Embodiment 3

[0057] Select 1000kg of waste tobacco stems produced by threshing and redrying plant, put it in the soaking tank, add 10000kg of water, water temperature 60℃, mix well, soak for 1 day, stir properly during this period to fully dissolve the solubles; then release the extract , Drain the material, send it to the squeezer for solid-liquid separation, combine the liquid with the aforementioned extract, and obtain a tobacco stem liquid culture medium after high temperature sterilization, for use.

[0058] Domestication and cultivation of Beauveria bassiana first-grade slant strain: Take a small amount of the above liquid medium, insert the Beauveria bassiana slant strain, and culture it on a shaker at 25°C for 3 days. Then transfer it to the same medium for culture, and transfer it continuously for 3 to 5 times in this way. Dilute the obtained culture solution and apply it to the tobacco stem waste solid medium (tobacco stem liquid medium plus 1.9% agar) Cultivate for 3 days at 25°C. ...

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PUM

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Abstract

The invention provides a tobacco matrix composite biopesticide and a preparation method thereof. The composite biopesticide which comprises at least a tobacco botanical insecticidal component and at least a microbial insecticidal component is prepared through fermenting insecticidal microbes with selecting a tobacco waste as a raw material. The preparation method comprises the following operationsteps: preparation of a medium, amplification culturing of spawns, fermentation of the biopesticide, and extraction. A fermentation technology can adopt liquid state fermentation or solid state fermentation. The tobacco matrix composite biopesticide of the invention which not only contains the insecticidal microbes and metabolites thereof, but also contains tobacco insecticidal components (nicotine and the like for example) has dual insecticidal effects. The method of the invention which allows resources of the tobacco waste which is not reasonably utilized at present to be fully utilized allows grain resources to be saved, and good economic benefits, ecological benefits and social benefits to be generated.

Description

Technical field [0001] The present invention relates to the technical field of biopesticide production, in particular to a method for producing biopesticides using biomass waste. In more detail, it relates to a tobacco matrix compound biological pesticide and a manufacturing method thereof. Background technique [0002] Biopesticides have the characteristics of wide sources of raw materials, safety to non-target organisms, low toxic and side effects, and good environmental compatibility. They have become a new trend in the development of the global pesticide industry. Therefore, the development of the biopesticides industry is very important to ensure the sustainable development of agriculture, protect people's lives and health, and protect the ecological environment. [0003] At present, biological pesticides are mainly produced by fermentation of agricultural and sideline products. The main raw materials are soybean cake powder, peanut cake powder, fish meal, rice bran, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/38A01N63/04A01N63/02A01N43/40A01N43/90A01P7/04
CPCY02A50/30
Inventor 朱大恒席宇杜雷朱润琪
Owner ZHENGZHOU UNIV
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