Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tobacco matrix composite biopesticide and preparation method thereof

A technology for biological pesticides and manufacturing methods, which can be applied in the fields of pesticides, botanical equipment and methods, biocides, etc., and can solve problems such as underutilization of resources.

Inactive Publication Date: 2013-09-25
ZHENGZHOU UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct use of tobacco waste as pesticides, the above resources are not fully utilized

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Select 1000kg of waste tobacco stems produced by threshing and redrying plant, place it in the soaking tank, add 10000kg of water, mix well, soak at room temperature for 2 days, stir appropriately during this period to fully dissolve the solubles; then release the extract, The material is drained and sent to the press for solid-liquid separation. The liquid is combined with the aforementioned extract, 0.5% peptone and 0.3% calcium carbonate are added, and the pH is adjusted to 7.2. After high temperature sterilization, the tobacco stem liquid medium is obtained. spare.

[0050] Domestication and cultivation of Bacillus thuringiensis (Bt) primary slant strains: Take a small amount of the above liquid medium, and insert Bacillus thuringiensis (Bt) slant strains into the tobacco stem liquid medium, and culture it with shaking at 28°C for 24 hours , And then transfer to the same medium for culture. Follow this method to transfer 3 to 5 times continuously. Dilute the obtained c...

Embodiment 2

[0055] According to the method in Example 1, the preparation of tobacco stem liquid medium and the expansion culture of Bacillus thuringiensis (Bt) secondary strains were carried out, and then solid-state fermentation was used to produce Bacillus thuringiensis (Bt) insecticide: 1000kg tobacco stem The waste material is broken to a particle size of 0.2-2cm, add 800kg of water, mix well, adjust the pH to 7.2, sterilize at 121°C for 20 minutes, and connect to Bacillus thuringiensis (Bt) secondary species after cooling (inoculation amount is 30%) , At a temperature of 28 ~ 35 ℃, aeration culture for 3 days to obtain the fermented material, the fermented material is dried and pulverized to obtain a powdered tobacco matrix Bacillus thuringiensis (Bt) compound insecticide, and its bacterial content is determined to be 1.5×10 10 Pieces / g, the total amount of tobacco insecticidal components is 12%.

Embodiment 3

[0057] Select 1000kg of waste tobacco stems produced by threshing and redrying plant, put it in the soaking tank, add 10000kg of water, water temperature 60℃, mix well, soak for 1 day, stir properly during this period to fully dissolve the solubles; then release the extract , Drain the material, send it to the squeezer for solid-liquid separation, combine the liquid with the aforesaid extract, and obtain the tobacco stem liquid medium after high temperature sterilization, which is ready for use.

[0058] Domestication and cultivation of Beauveria bassiana first-grade slant strain: Take a small amount of the above liquid medium, insert the Beauveria bassiana slant strain, and culture it on a shaker at 25°C for 3 days. Then transfer it to the same medium for culture, and transfer it continuously for 3 to 5 times in this way. Dilute the obtained culture solution and apply it to the tobacco stem waste solid medium (tobacco stem liquid medium plus 1.9% agar) Cultivate for 3 days at 25...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a tobacco matrix composite biopesticide and a preparation method thereof. The composite biopesticide which comprises at least a tobacco botanical insecticidal component and at least a microbial insecticidal component is prepared through fermenting insecticidal microbes with selecting a tobacco waste as a raw material. The preparation method comprises the following operationsteps: preparation of a medium, amplification culturing of spawns, fermentation of the biopesticide, and extraction. A fermentation technology can adopt liquid state fermentation or solid state fermentation. The tobacco matrix composite biopesticide of the invention which not only contains the insecticidal microbes and metabolites thereof, but also contains tobacco insecticidal components (nicotine and the like for example) has dual insecticidal effects. The method of the invention which allows resources of the tobacco waste which is not reasonably utilized at present to be fully utilized allows grain resources to be saved, and good economic benefits, ecological benefits and social benefits to be generated.

Description

Technical field [0001] The invention relates to the technical field of production of biological insecticides, in particular to a method for producing biological insecticides by using biomass waste. In more detail, it relates to a tobacco matrix composite biological insecticide and a manufacturing method thereof. Background technique [0002] Biological pesticides have a wide range of raw materials for production, are safe to non-target organisms, have low toxic and side effects, and are compatible with the environment. They have become a new trend in the development of the global pesticide industry. Therefore, the development of the biopesticide industry is very important for ensuring the sustainable development of agriculture, protecting people's lives and health, and protecting the ecological environment. [0003] At present, biological pesticides are mainly produced by fermentation of agricultural and sideline products. The main raw materials are soybean meal, peanut meal, fish...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/38A01N63/04A01N63/02A01N43/40A01N43/90A01P7/04
CPCY02A50/30
Inventor 朱大恒席宇杜雷朱润琪
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com