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Plant and microbe composite pesticide, and preparation method thereof

A technology of microorganisms and pesticides, which is applied in the field of compound pesticides of plants and microorganisms and its preparation, can solve the problems that are not involved in the fermentation process of tobacco, singleness, etc., and achieve pollution-free, safe and reliable, broad application prospects, and a wide range of applications Effect

Inactive Publication Date: 2019-09-06
LIUYANG BRANCH OF CHANGSHA COMPANY OF HUNAN TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above patent application involves tobacco, it does not involve the fermentation process of tobacco, which belongs to the category of single botanical pesticides

Method used

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  • Plant and microbe composite pesticide, and preparation method thereof
  • Plant and microbe composite pesticide, and preparation method thereof
  • Plant and microbe composite pesticide, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A compound insecticide for plants and microorganisms, comprising the following raw materials in parts by weight:

[0059] Plants: 20 parts tobacco, 4 parts Chinese cedar, 4 parts marigold;

[0060] Bacteria solution: 0.1 part of Paecilomyces solution, 0.1 part of Ascospora solution, 0.5 part of Penicillium solution, and 0.5 part of Bacillus sphaericus solution;

[0061] 40 parts of water.

[0062] The preparation method is as follows:

[0063] 1) The preparation method of the caterpillar bacterium liquid or the bacillus sphaericus liquid:

[0064] Inoculate the purchased Penicillium spp. or Bacillus sphaericus in the beef extract peptone liquid medium respectively, and culture at 30°C and 60r / min for 20h;

[0065] 2) The preparation method of Paecilomyces liquid or Ascospora liquid:

[0066] Inoculate the purchased Paecilomyces or Ascosporium into PS medium + 1% egg yolk liquid medium, culture at 25°C with shaking at 100r / min for 3 days;

[0067] 3) Preparation of ...

Embodiment 2

[0073] A compound insecticide for plants and microorganisms, comprising the following raw materials in parts by weight:

[0074] Plants: 35 parts of tobacco, 7 parts of cedarwood, 7 parts of marigold;

[0075] Bacteria liquid: 0.3 part of Paecilomyces liquid, 0.3 part of Ascospora liquid, 1.0 part of Penicillium liquid, 1.0 part of Bacillus sphaericus liquid;

[0076] 60 parts of water.

[0077] The preparation method is as follows:

[0078] 1) The preparation method of the caterpillar bacterium liquid or the bacillus sphaericus liquid:

[0079] Inoculate the purchased Penicillium spp. or Bacillus sphaericus into the beef extract peptone liquid medium respectively, and incubate at 35°C with shaking at 90r / min for 36h;

[0080] 2) The preparation method of Paecilomyces liquid or Ascospora liquid:

[0081] Inoculate the purchased Paecilomyces or Ascosporium into PS medium + 1% egg yolk liquid medium respectively, and culture at 27°C with shaking at 120r / min for 4 days;

[0...

Embodiment 3

[0088] A compound insecticide for plants and microorganisms, comprising the following raw materials in parts by weight:

[0089] Plants: 40 parts of tobacco, 10 parts of three-pointed cedar, 10 parts of marigold;

[0090] Bacteria liquid: 0.5 part of Paecilomyces liquid, 0.5 part of Ascospora liquid, 1.5 parts of Penicillium liquid, 1.5 parts of Bacillus sphaericus liquid;

[0091] 80 parts of water.

[0092] The preparation method is as follows:

[0093] 1) The preparation method of the caterpillar bacterium liquid or the bacillus sphaericus liquid:

[0094] Inoculate the purchased Penicillium spp. or Bacillus sphaericus in the beef extract peptone liquid medium respectively, and culture at 40°C and 120r / min shaking for 48h;

[0095] 2) The preparation method of Paecilomyces liquid or Ascospora liquid:

[0096] Inoculate the purchased Paecilomyces or Ascosporium into PS medium + 1% egg yolk liquid medium respectively, and culture at 30°C with shaking at 150r / min for 5 day...

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PUM

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Abstract

The invention discloses a plant and microbe composite pesticide, and a preparation method thereof. The plant and microbe composite pesticide comprises, by weight, a plant component composed of 20 to 50 parts of tobacco leaf, 4 to 10 parts of cephalotaxus fortunei, and 4 to 10 parts of Aztec marigold; a bacterium solution composed of 0.1 to 0.5 part of paecilomyces varioti bacterium solution, 0.1 to 0.5 part of Aschersonia bacterium solution, 0.5 to 1.5 parts of insecticidal bacteria qingchongjun solution, and 0.5 to 1.5 parts of Bacillus sphaericus solution; and 40 to 80 parts of water. The preparation method comprises following steps: fungus and bacteria are subjected to independent culture and breeding to prepare the bacterium solution; the plant raw materials are subjected to treatmentincluding washing and crushing; the plant raw materials are introduced into a fermentation tank, and the fermentation tank is inoculated with the bacterium solution for fermentation;and after fermentation, filtering is carried out so as to obtain the plant and microbe composite pesticide. The plant and microbe composite pesticide is high in efficiency, low in toxicity, and wide in insecticidal range, is harmless for human, domestic animals, and the environment, and can be applied on rice, fruits, and economic crops.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a plant-microbe composite pesticide and a preparation method thereof. Background technique [0002] With the improvement of living standards, people's demand for pollution-free melons, fruits, vegetables and other crops has increased sharply. However, traditional chemical pesticides tend to leave toxins on the surface of crops and are difficult to remove. Therefore, biopesticides have become one of the most active fields in agricultural bioengineering research due to their high efficiency, low toxicity and no residue in the past ten years. At present, chemical pesticides are decreasing at a rate of 2% per year, while biological pesticides are increasing at a rate of 5%. [0003] Tobacco contains a variety of natural insecticidal ingredients, such as tobacco alkaloids, tobacco essential oils, etc., which can be used as pollution-free botanical insecticides. Moreover, the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/38A01N63/04A01N63/00A01N63/02A01P5/00A01P7/04
CPCA01N65/00A01N65/38A01N65/12A01N65/06A01N63/00A01N63/30A01N63/10Y02A50/30
Inventor 曾维爱谢鹏飞蔡海林谭琳翟争光向世鹏戴杏华赵阿娟唐春闺程予奇
Owner LIUYANG BRANCH OF CHANGSHA COMPANY OF HUNAN TOBACCO
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