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Bacillus subtilis, preparation, preparation method of preparation, and application of bacillus subtilis and preparation

A Bacillus subtilis and preparation technology, applied in the field of biological pesticides, can solve the problems of poor drug resistance and safety, poor antibacterial effect, and need to improve the killing or inhibition effect, and achieve a significant control effect

Active Publication Date: 2012-07-04
高丙利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as the Chinese invention patent whose application number is 200910148250.9 discloses a Bacillus subtilis microbial inoculum and a composite microbial inoculum, which can inhibit pathogenic bacteria such as eggplant sclerotinia and tomato Botrytis cinerea, overcome In the prior art, chemical pesticides have defects such as poor environmental protection, easy to produce drug resistance and poor safety, so as to realize the advantages of good environmental protection, not easy to produce drug resistance and good safety
The microbial agent and the composite microbial agent have a wide antibacterial spectrum, but the antibacterial effect is not very good, especially the killing or inhibiting effect on crop molds, especially downy mildew, needs to be improved

Method used

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  • Bacillus subtilis, preparation, preparation method of preparation, and application of bacillus subtilis and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A wettable powder prepared by:

[0032] 1. Liquid fermentation culture

[0033] ①Strain activation

[0034] After the strain was taken out from the -70°C refrigerator, it was activated and cultured through 3 transfers, and the medium was liquid seed medium PSA;

[0035] ②Preparation of seed solution

[0036] Pick a single colony and culture it in a new liquid seed medium PSA at 28°C and 180rpm for 15 hours;

[0037] ③ Inoculate the cultured liquid bacteria into the liquid medium at an inoculation amount of 0.5%;

[0038] ④ 200rpm, cultured at 20°C for 3 days;

[0039] ⑤ The fermented liquid obtained through step ④ is fermented and cultivated to obtain mother powder by drying, then adding carrier fillers, auxiliary agents, stabilizers and protective agents in proportion, and crushing to obtain the preparation;

[0040] The formula of the liquid medium is: by weight percentage, maltose 0.2%; corn flour, 0.5%; soybean flour, 0.4%; tryptone, 0.5%; CaCl 2 , 0.02%; MnSO...

Embodiment 2

[0047] A wettable powder prepared by:

[0048] 1. Liquid fermentation culture

[0049] ①Strain activation

[0050] After the strain was taken out from the -70°C refrigerator, it was activated and cultured through 3 transfers, and the medium was liquid seed medium LB;

[0051] ②Preparation of seed solution

[0052] Pick a single colony and culture it in a new liquid seed medium LB at 30°C and 200rpm for 18 hours;

[0053] ③Inoculate the cultured liquid bacteria into the liquid culture medium according to the inoculum amount of 2%;

[0054] ④ 230rpm, cultured at 25°C for 2 days;

[0055] ⑤ The fermented liquid obtained through step ④ is fermented and cultivated to obtain mother powder by drying, and then adding carrier fillers, auxiliary agents, stabilizers and protective agents in proportion and pulverizing to obtain the preparation;

[0056]The formula of the liquid medium is: by weight percentage, maltose 0.3%; corn flour, 0.6%; soybean flour, 0.6%; tryptone, 0.8%; CaCl ...

Embodiment 3

[0063] A wettable powder prepared by:

[0064] 1. Liquid fermentation culture

[0065] ①Strain activation

[0066] After the strain was taken out from the -70°C refrigerator, it was activated and cultured through 3 transfers, and the medium was liquid seed medium PSA;

[0067] ②Preparation of seed solution

[0068] Pick a single colony and culture it in a new liquid seed medium PSA at 35°C and 220rpm for 20 hours;

[0069] ③ Inoculate the cultured liquid bacteria into the liquid culture medium at an inoculum volume of 4%;

[0070] ④ 250rpm, culture at 30°C for 1 day;

[0071] ⑤ The fermented liquid obtained through step ④ is fermented and cultivated to obtain mother powder by drying, and then adding carrier fillers, auxiliary agents, stabilizers and protective agents in proportion and pulverizing to obtain the preparation;

[0072] The formula of the liquid medium is: by weight percentage, maltose 0.4%; corn flour, 0.8%; soybean flour, 0.9%; tryptone, 1.0%; CaCl 2 , 0.08...

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Abstract

The invention relates to a bacillus subtilis strain, a preparation prepared from the bacillus subtilis strain, a preparation method of the preparation, application of the bacillus subtilis strain to preparation of a preparation for preventing and treating crop mycete diseases and application of the preparation to prevention and treatment of the crop mycete diseases, and belongs to the technical field of biopesticide. The bacillus subtilis with the biological name of bacillus subtilis was collected in China General Microbiological Culture Collection Center (CGMCC) on December 26, 2012 (the address is Rentun Road, Chaoyang District, Beijing and the postal code is 100101), wherein the collection number is CGMCC No.5652. An in vitro living plant test shows that the bacillus subtilis preparation has a certain effect of preventing and treating crop mycete diseases and particularly has an obvious effect of preventing and treating peronospora sparsa disease of crops.

Description

technical field [0001] The invention relates to a strain of Bacillus subtilis, a preparation made by using the strain, a preparation method of the preparation, an application of the strain in the preparation of a preparation for preventing and treating fungal diseases of crops, and the use of the preparation in preventing and controlling crop fungal diseases. The application of the mold disease aspect belongs to the technical field of biological pesticides. Background technique [0002] With people's increasing concern for food safety and environmental protection, "organic agriculture" has become the development direction of agriculture, and the development of low-toxic, high-efficiency, low-residue pure natural biological pesticides has become a new direction of recent pesticide research. However, due to the limitations of biological pesticides, they usually have low drug efficacy, slow action and high price, so they are difficult to be accepted by farmers. [0003] Bacill...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N25/14A01P3/00C12R1/125
Inventor 高丙利沈婷婷张鹏荣跃文张海风
Owner 高丙利
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