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Bacillus methylotrophicus wettable powder and preparation method and application thereof

A methylotrophic, wettable powder technology, applied in the direction of botany equipment and methods, applications, fungicides, etc., can solve problems such as poor control effects, overcome environmental toxic side effects and pesticide residue problems, and reduce production costs , the effect of energy consumption reduction

Active Publication Date: 2014-12-24
NORTH CHINA PHARMA GROUP AINO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methylotrophic Bacillus wettable powder of the invention is mainly aimed at citrus diseases, and has poor control effects on common cucumber gray mold and apple rot in vegetable cultivation in protected areas.

Method used

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  • Bacillus methylotrophicus wettable powder and preparation method and application thereof
  • Bacillus methylotrophicus wettable powder and preparation method and application thereof
  • Bacillus methylotrophicus wettable powder and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of methylotrophic bacillus wettable powder WP1-1

[0049] Add 1.0% diatomaceous earth to 1 part of the fermentation broth containing methylotrophic bacillus, collect the bacteria through the solid-liquid separation step, and dry to moisture at 60°C-70°C and vacuum degree of 0.07Mpa-0.09Mpa ≤5.0%, that is, the original drug of methylotrophic bacillus.

[0050] Add 6 parts by mass of GSWP compound additives and 6 parts by mass of dispersant MF to 100 parts by mass of the original drug of methylotrophic bacillus, mix evenly, and then perform jet milling. The crushing fineness is 45 μm. %, to obtain methylotrophic bacillus wettable powder WP1-1.

[0051] (2) Preparation of methylotrophic bacillus wettable powder WP1-2~WP1-8

[0052] The preparation process is basically the same as the preparation of methylotrophic bacillus wettable powder WP1-1, the difference is: firstly add flocculant polyacrylamide to the fermentation liquid containing methylotrophic ba...

Embodiment 2

[0064] (1) Preparation of methylotrophic bacillus wettable powder WP2-1

[0065] Add 300ppm polyacrylamide to 1 part of the fermentation broth containing methylotrophic bacillus. After the cells are coagulated for 30 minutes, carry out solid-liquid separation and collect the cells without adding diatomaceous earth. °C and vacuum degree of 0.07Mpa to 0.09Mpa and dried until the water content is ≤5.0%, the original drug of methylotrophic bacillus can be obtained.

[0066]Add 6 parts by mass of GSWP compound additives and 6 parts by mass of dispersant MF to 100 parts by mass of the original drug of methylotrophic bacillus, mix evenly, and then perform jet milling. The crushing fineness is 45 μm. %, to obtain methylotrophic bacillus wettable powder WP2-1.

[0067] (2) Preparation of methylotrophic bacillus wettable powder WP2-2~WP2-8

[0068] The preparation process is basically the same as that of the methylotrophic bacillus wettable powder WP2-1, the difference is that after t...

Embodiment 3

[0077] (1) Preparation of methylotrophic bacillus wettable powder WP3-1

[0078] Add 300ppm polyacrylamide to 1 part of the fermentation broth containing methylotrophic bacillus, add 1.0% diatomaceous earth after the cells are coagulated for 30 minutes, collect the cells through the solid-liquid separation step, and store them at room temperature under a vacuum of 0.07 Dry under the condition of MPa~0.09Mpa for 6 hours to obtain the original drug of methylotrophic bacillus.

[0079] Add 6 parts by mass of GSWP compound additives and 6 parts by mass of dispersant MF to 100 parts by mass of the original drug of methylotrophic bacillus, mix evenly, and then perform jet milling. The crushing fineness is 45 μm. %, to obtain methylotrophic bacillus wettable powder WP3-1.

[0080] (2) Preparation of methylotrophic bacillus wettable powder WP3-2~WP3-7

[0081] The preparation process is basically the same as the preparation of the methylotrophic bacillus wettable powder WP3-1, the d...

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Abstract

The invention discloses bacillus methylotrophicus wettable powder which comprises 100 parts by mass of bacillus methylotrophicus active components, 4-10 parts by mass of a wetting agent and 4-10 parts by mass of a dispersing agent. A method for preparing the bacillus methylotrophicus wettable powder comprises the following steps: weighing the bacillus methylotrophicus active ingredients, the wetting agent and the dispersing agent according to a ratio, and uniformly mixing; and milling through a jet mill, thereby obtaining the bacillus methylotrophicus wettable powder. The wettable powder is stable in quality and convenient to preserve and use for a long time, has good effects of preventing and treating cucumber gray mold and apple rot, solves the problems such as environmental toxic and side effects and pesticide residues brought by chemical agents, has the characteristics of high efficiency, no toxicity, safety and no residue, is a safe, effective, low-residue and environment-friendly microbial pesticide and has wide application prospects in the field of microbial control.

Description

technical field [0001] The invention relates to a methylotrophic bacillus wettable powder and a preparation method and application thereof, belonging to the field of microbial pesticides. Background technique [0002] Microbial pesticides refer to the use of living microorganisms and their metabolites to prevent and control agricultural pests and diseases. Microbial pesticides have strong selectivity, are safe to humans, livestock, crops and the natural environment, and are not easy to produce drug resistance, so they have been extensively studied. At present, there are relatively mature microbial pesticide products on the market, such as Bacillus thuringiensis, Bacillus licheniformis, and Bacillus subtilis. [0003] With the rapid development of economic globalization, my country will face increasingly stringent pesticide residue standards in international agricultural products and food trade, which requires my country's agricultural products to gradually transition from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/14A01N63/00A01P3/00
Inventor 杨德昌李学红胡江春李春田潘华奇刘书琴杨永军赵少品王晓宏胡艳霞苑丽蒲
Owner NORTH CHINA PHARMA GROUP AINO
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