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Goffer pseudomonas P94 and application thereof

A technology of pseudomonas and folds, applied in the field of new microbial strains, to achieve good control effects, good plant growth promotion effects, and increase yield effects

Inactive Publication Date: 2007-08-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad on the use of Pseudomonas rugosa for the control of Botrytis cinerea

Method used

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  • Goffer pseudomonas P94 and application thereof
  • Goffer pseudomonas P94 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Identification of Example 1 P94

[0025] Pseudomonas corrugata (Pseudomonas corrugata) P94 CGMCC NO.1895 was identified as Pseudomonas corrugata genotype II based on the results of morphological characteristics, physiological and biochemical characteristics, 16SrDNA sequence and specific primer analysis. The specific identification results are as follows:

[0026] 1. Morphological characteristics of bacteria

[0027] Gram staining is negative, yellow-green soluble non-fluorescent pigments are produced on PDA medium, and the colonies are blue-green; on KB medium, less pigment is produced, and the colonies are light yellow-green. Electron microscope observation shows that the bacteria are oblong, 0.7×1.8 μm in size, and have no less than two polar flagella (see Figure 1).

[0028] 2. Physiological and biochemical characteristics

[0029] Table 1 shows the physiological and biochemical characteristics of Pseudomonas rugosa P94.

[0030] Table 1. Physiological and bioch...

Embodiment 2

[0038] Embodiment 2 antibacterial spectrum analysis

[0039] Plate inhibition detection method for pathogenic fungi: use a hollower to inoculate target pathogenic fungi with a diameter of 5mm on the center of the PDA plate, P94 and target bacteria at an angle of 180° and inoculate at 2.5cm from both sides of the target pathogenic fungus, and incubate at 25°C for 4 to The diameter of pathogenic fungal colony was measured after 6 days. The plate inoculated with target fungi but not P94 was used as a control. The growth inhibition rate was calculated according to the following formula:

[0040] Growth inhibition rate=(C-T) / C×100%

[0041] C: Control fungal growth diameter

[0042] T: fungal growth diameter after inoculation with P94

[0043] Inhibition detection method on pathogenic bacteria plates: double-layer culture method was used to detect the inhibition of Pseudomonas rugosa P94 on pathogenic bacteria. After activating P94 with KB medium, make 10 with physiological sa...

Embodiment 3

[0051] Example 3 Detection of biocontrol-related traits

[0052] Protease detection: use skim milk plate (tryptone 5g, yeast extract 2.5g, glucose 1g, 7% skim milk 250ml, agar 15g, make up to 1000ml with water) to detect protease. Inoculate P94 into the center of the plate and incubate at 28°C for 72 hours. A transparent circle around the colony indicates protease positive (see Figure 3).

[0053] HCN detection: HCN detection test paper preparation, 10mg copper (II) ethylacetoacetate and 10mg 4,4'-methylenebis-(N,N-dimethylaniline) dissolved in 4ml of chloroform, cut Whatman filter paper strips, soaked in the prepared solution In the process, the HCN detection test paper was prepared after the chloroform volatilized completely. Inoculate P94 in a centrifuge tube containing 1ml of KB medium, suspend the HCN detection test paper in the centrifuge tube, and incubate at 28°C for 36 hours. The HCN detection test paper turns blue, indicating that P94 produces HCN (see Figure 4).

...

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Abstract

The invention discloses a new Pseudomonas corrugate strain (P94 GMCC No.1895), which is characterized by the following: manufacturing relative HCN to prevent bacteria, proteinase and phospholipase and IAA; inhibiting partial plant pathogenic fungus (Botrytis cinerea, Ceratocystis fimbriata, Monilinia laxa, Magnaporthe grisea, Pythium aphanidermatum and Phytophthora capsici) and partial pathogenic bacteria (Pseudomonas syringae, Acidovorax avenae and Ralstonia solanacearum); accelerating the growth of tomato and cucumber to improve production by 22%-28%.

Description

technical field [0001] The invention relates to a new microbial strain and its application, specifically Pseudomonas rugosa P94 and its application in plant growth promotion and disease biological control. Background technique [0002] Vegetable gray mold caused by Botrytis cinerea infection is an important plant disease with global distribution. Great losses are caused to the output of solanaceous fruits, berries, and melons. Since the 1980s, with the continuous expansion of protected areas in my country, the damage of botrytis cinerea has become increasingly serious. The microclimate environment of the greenhouse is very conducive to the occurrence of vegetable gray mold, which has become one of the most important diseases of vegetables in protected areas in my country. The pathogen Botrytis mostly invades from the open residual flowers, causing the petals to rot, and a gray-brown mold layer grows, and then expands to the young fruit. Causes more than half of the fruit ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12R1/38
Inventor 王慧敏郭岩彬陈凡郑辉王建辉杨毅玲李金云王远宏
Owner CHINA AGRI UNIV
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