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Yersinia pseudotuberculosis-sourced antifungal protein and coding gene and application thereof

A Yersinia, antifungal protein technology, applied in the field of DNA sequences encoding antifungal proteins, to achieve broad-spectrum antifungal activity

Inactive Publication Date: 2018-09-04
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no report has been reported to obtain antifungal proteins and their genes with deoxyribonuclease activity from bacteria

Method used

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  • Yersinia pseudotuberculosis-sourced antifungal protein and coding gene and application thereof
  • Yersinia pseudotuberculosis-sourced antifungal protein and coding gene and application thereof
  • Yersinia pseudotuberculosis-sourced antifungal protein and coding gene and application thereof

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Experimental program
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Embodiment Construction

[0040] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0041] Construction of YPK_AFP1001 inducible expression vector

[0042] The new gene ypk_ypk_AFP1001 was obtained, and the primer sequence of ypk_ypk_AFP1001 was amplified from wild-type Yersinia pseudotuberculosis (Yersiniapseudotuberculosis YPIII) genomic DNA using specific primers as follows

[0043] Forward primer YPK_AFP1001-F:

[0044] 5'-CGCGGATCCATGAAACCGTTAGATCAGAATATTA-3'

[0045] Reverse primer YPK_AFP1001-R:

[0046] 5'-CCGCTCGAGTTAACATCCTCCGCCACCTTTTGAG-3'

[0047] (1) Amplification of YPK_AFP1001 gene

[0048] Table 1 YPK_AFP1001 gene PCR amplification system

[0049]

[0050] PCR program:

[0051]

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Abstract

The invention discloses yersinia pseudotuberculosis-sourced antifungal protein and a coding gene and application thereof, and belongs to the technical field of genetic engineering. The antifungal protein is protein comprising the amino acid sequence shown as SEQ ID NO: 1, or protein obtained by substituting, deleting or inserting one or several amino acid sequences in the amino acid sequence shownas SEQ ID NO: 1 and having equivalent activity. The invention further provides the coding gene, an expression vector and engineering bacteria of the antifungal protein. The invention further providesa separation and purification method of the yersinia pseudotuberculosis-sourced antifungal protein. The protein is applied to an antifungal agent and the coding gene of the protein is applied to a transformed plant. The YPK_AFP1001 antifungal gene and the protein, related by the invention, have higher and more broad-spectrum antifungal activity.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a Yersinia pseudotuberculosis antifungal protein and its coding gene and application, in particular, to an antifungal protein YPK_AFP1001 derived from Yersinia pseudotuberculosis (Yersinia pseudotuberculosis) , methods of production and use thereof and DNA sequences encoding antifungal proteins. Background technique [0002] Pathogenic fungi can cause devastating damage to crops and have adverse health consequences for livestock and humans (Wang et al. 2003; Yevtushenko et al. 2005; Corrado et al. 2008); fungal infections directly cause five major food crops each year Yield losses of crops rice, wheat, maize, potatoes and soybeans amounted to 125 million tonnes. The diseases of fungi infecting food crops mainly include: rice blast, wheat stem rust, corn smut, potato late blight and soybean rust and other crop diseases. Today, the control of plant diseases mainly relie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/22C12N15/55A01N47/44A01P3/00A23L3/3526
CPCA01N47/44A23L3/3526C12N9/22
Inventor 沈锡辉潘君风宋莉杨昶杏徐磊张磊王瑶
Owner NORTHWEST A & F UNIV
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