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Chrysalid pteromalid antibiotic protein Pp-AP3 and encoding nucleic acid sequence thereof

A technology of antibacterial protein and nucleic acid sequence, which is applied in the fields of genetic engineering and molecular biology, and can solve the problem of reporting antibacterial peptides

Inactive Publication Date: 2008-12-24
ZHEJIANG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Many researchers have discovered hundreds of antimicrobial proteins from different insects, but there is no literature report on the antimicrobial peptides isolated from the chrysalis golden wasp

Method used

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  • Chrysalid pteromalid antibiotic protein Pp-AP3 and encoding nucleic acid sequence thereof
  • Chrysalid pteromalid antibiotic protein Pp-AP3 and encoding nucleic acid sequence thereof
  • Chrysalid pteromalid antibiotic protein Pp-AP3 and encoding nucleic acid sequence thereof

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Embodiment 1

[0095] Embodiment 1: (with reference to figure 1 :)

[0096] 1. Construction of Chrysalis chrysalis cDNA (complementary DNA) expression library:

[0097] Chrysalis chrysalis came from the Laboratory of Insect Physiology and Biochemistry, Institute of Insect Science, Zhejiang University.

[0098] a. Isolate total RNA from the whole body of Chrysalis chrysalis, obtain poly(A)+RNA with purified mRNA kit, and then use cDNA synthesis kit, Synthesis kits and A cDNA (complementary DNA) expression library was constructed using the Gigapack III cloning kit (purchased from the Takana agency of American clontech company, without a Chinese name).

[0099] b. According to the operation instructions of the kit, each step is performed to construct a cDNA (complementary DNA) expression library.

[0100] 2. Screening of Chrysalis chrysalis cDNA (complementary DNA) expression library:

[0101] a. Prepare SOLR (a type of Escherichia coli, no Chinese name) bacterial solution: burn the inoc...

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Abstract

The invention discloses a nucleic acid sequence of a pteromalus puparum antifungal protein Pp-AP3 code. The separated DNA molecule comprises: a polypeptide nucleotide sequence with the pteromalus puparum antifungal protein Pp-AP3 activity is encoded, the nucleotide sequence and the nucleotide sequence from the 767th ribonucleotide to the 871th ribonucleotide in the SEQ ID NO:1 have 70 percent of homology; or the nucleotide sequence can be hybridized with the nucleotide sequence from the 767th ribonucleotide to the 871th ribonucleotide in the SEQ ID NO:1 at a temperature of between 40 and 55 DEG C. The invention also discloses a pteromalus puparum antifungal protein polypeptide Pp-AP3 which has an amino acid sequence shown in the SEQ ID NO: 2. The pteromalus puparum antifungal protein Pp-AP3 has the antibiosis performance.

Description

technical field [0001] The invention relates to the fields of molecular biology and genetic engineering. In particular, it is an antibacterial protein Pp-AP3 expressed in the chrysalis chrysalis and its encoded nucleic acid sequence. Background technique [0002] In recent years, due to the increasing problems of drug abuse, drug residues and bacterial resistance, existing antibiotics are losing their original efficacy, which has aroused people's attention to food safety, and more and more countries have begun to call for banning Antibiotics, and antibacterial peptides (antibacterial peptides) have aroused great research interest because of their unique biological activity and special mechanism of action different from traditional antibiotics, and have become one of the hot spots in the field of molecular biology and biochemistry research. Researchers from various countries have discovered hundreds of antimicrobial peptides from mammals, insects, amphibians, etc., some of w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435A23K1/16A61K38/17A61P31/00A23K20/153
Inventor 慎小晶叶恭银成雄鹰胡萃
Owner ZHEJIANG UNIV
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