Process for purifying bromelain by chelated metal affinity membrane
A technology of bromelain and metal chelation, applied in the direction of hydrolytic enzymes, etc., can solve the problems of low product purity, high cost, short diffusion path, etc., achieve the effect of high purification multiple and enzyme activity, convenient source of raw materials, and easy extraction and purification
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Activation of nylon membrane and modification of bonded hydroxyethyl cellulose, the specific steps are as follows:
[0028] (1) Nylon membranes (10 sheets) were first hydrolyzed in 1 mol / L HCl at 25°C for 24 hours, and then activated with formaldehyde. Ten hydrolyzed nylon membranes were immersed in 20ml of formaldehyde solution (>36.5wt.%), added with 0.2ml of phosphoric acid (85wt.%), reacted at 60°C for 7h, and washed with hot water at 40-50°C for several times.
[0029] (2) Immerse the above-mentioned formaldehyde-activated nylon membranes (10 sheets) in 100ml of 2% hydroxyethyl cellulose solution, react at room temperature for 1h, then transfer to a 90°C oven, take it out after 1h, and wash with 1% Triton La (X-100) NaOH solution (0.1mol / L) was used to wash away unreacted hydroxyethyl cellulose, and then washed in deionized water for 2 days.
Embodiment 2
[0031] The modified nylon membrane chelates metal zinc ions, and the specific steps are as follows:
[0032] (1) 100 sheets (about 10 grams) of nylon membranes after the above-mentioned modification were immersed in 4.6ml epichlorohydrin, 46ml 2mol / L sodium hydroxide and 5mmol sodium borohydride mixed solution, reacted at room temperature for 2 hours, and then directly applied to Add 23ml of epichlorohydrin and 46ml of 2mol / L sodium hydroxide to the mixed solution, react at 60°C for 12h, and wash with deionized water several times.
[0033] (2) Immerse 10 films (approximately 1 gram) of the above-mentioned combined cyclopropane into a mixed solution containing 86mmol of iminodiacetic acid (IDA), 8mmol of sodium borohydride and 114.4ml of 2mol / L sodium carbonate, and shake at a constant temperature of 60°C After reacting for 12 hours, wash with deionized water, 5vol.% acetic acid and deionized water several times.
[0034] (3) Immerse the above-mentioned film in 500ml of 100pp...
Embodiment 3
[0036] Use a metal chelate membrane to adsorb bromelain and optimize the conditions. The specific steps are as follows:
[0037] (1) Determination of reaction time. Stack 10 modified nylon membranes to form a membrane stack, put them into the membrane bridge, and send 100ml of 1.0mg / ml bromelain Tris-HCl solution (pH=8) with a peristaltic pump at a flow rate of 2.0ml / min. 10min, 20min, 30min, 40min, 50min, 60min, 80min and 100min, carry out adsorption, and measure the amount of bromelain adsorbed. Experimental results such as figure 1 , the optimal response time is selected at 60min.
[0038] (2) Determination of reaction pH. Stack 10 modified nylon membranes to form a membrane stack, put them into the membrane bridge, and send 100ml of 1.0mg / ml bromelain Tris-HCl solution with different pH values into it with a peristaltic pump, and circulate the reaction for 2 hours at a flow rate of 2.0ml / min , adding bromelain in Tris-HCl solution with pH values of 5.0, 6.0, 7.0, 8...
PUM
Property | Measurement | Unit |
---|---|---|
quality score | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com