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Preparation method of graphene microsphere biological separation medium with controllable particle size

A technology of bioseparation and olefin microspheres, which is applied in chemical instruments and methods, and other chemical processes, can solve the problems of cumbersome operation, low sample activity recovery rate, and unsatisfactory purification effect, etc., and achieve high adsorption capacity and facilitate large-scale The effect of extraction and purification, convenient sample source

Active Publication Date: 2015-07-08
佛山市富馨科技实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have certain advantages, they all have disadvantages such as cumbersome operation, low recovery rate of sample activity, and unsatisfactory purification effect, especially when extracting a small amount of biologically active substances from a large volume of dilute solution.

Method used

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  • Preparation method of graphene microsphere biological separation medium with controllable particle size
  • Preparation method of graphene microsphere biological separation medium with controllable particle size
  • Preparation method of graphene microsphere biological separation medium with controllable particle size

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Add graphene oxide (44.55% molar oxygen content) into deionized water to form a mixed solution, and the mass concentration of graphene oxide in the mixed solution is 0.025wt%. Stirring under a magnetic stirrer for 20 hours, the rotating speed is 1200r / min;

[0040] (2) Put the mixed solution into an ultrasonic machine, and after ultrasonic vibration for 10 hours, the graphene oxide flakes are sufficiently dispersed, cooled in ice water, and the temperature of the water in the ultrasonic machine is 20°C;

[0041] (3) The mixed solution is placed in the atomizer, and it is atomized into aerosol droplets. 2 Driven by a suction and filtration vacuum pump, it slowly passes through a quartz tube heated to 400°C, and the end is collected by a PTFE filter membrane with a pore size of 0.05um;

[0042] (4) The collected film was dried at room temperature for 6 hours, and then placed in a vacuum dryer at 60° C. for 24 hours to obtain graphene microspheres with a controllable ...

Embodiment 2

[0045] (1) Add graphene oxide (44.55% molar oxygen content) into deionized water to form a mixed solution, and the mass concentration of graphene oxide in the mixed solution is controlled to be 0.05wt%. Stirring under a magnetic stirrer for 20 hours, the rotating speed is 1200r / min;

[0046] (2) Put the mixed solution into an ultrasonic machine, and after ultrasonic vibration for 10 hours, the graphene oxide flakes are sufficiently dispersed, cooled in ice water, and the temperature of the water in the ultrasonic machine is 20°C;

[0047] (3) The mixed solution is placed in the atomizer, and it is atomized into aerosol droplets. 2 Driven by a vacuum pump and a suction filter, it slowly passes through a quartz tube heated to 400°C, and the end is collected with a 0.1um PTFE filter membrane;

[0048] (4) The collected film was dried at room temperature for 6 hours, and then placed in a vacuum dryer at 60° C. for 24 hours to obtain graphene microspheres with a controllable parti...

Embodiment 3

[0051] (1) Add graphene oxide (46.88% molar oxygen content) into deionized water to form a mixed solution, and the mass concentration of graphene oxide in the mixed solution is controlled to be 0.05wt%. Stirring under a magnetic stirrer for 20 hours, the rotating speed is 1200r / min;

[0052] (2) Put the mixed solution into an ultrasonic machine, and after ultrasonic vibration for 10 hours, the graphene oxide flakes are sufficiently dispersed, cooled in ice water, and the temperature of the water in the ultrasonic machine is 20°C;

[0053] (3) The mixed solution is placed in the atomizer, and it is atomized into aerosol droplets. 2 Driven by a vacuum pump and a suction filter, it slowly passes through a quartz tube heated to 450°C, and the end is collected with a 0.1um PTFE filter membrane;

[0054] (4) The collected film was dried at room temperature for 6 hours, and then placed in a vacuum dryer at 60° C. for 24 hours to obtain graphene microspheres with a controllable parti...

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Abstract

The invention relates to a preparation method of a graphene microsphere biological separation medium with a controllable particle size. The preparation method comprises the following steps: uniformly stirring and mixing graphene oxide and deionized water to obtain a mixed solution; performing ultrasonic treatment and cooling on the mixed solution, then atomizing into aerosol droplets, passing the aerosol droplets through a quartz tube, collecting by using a polytetrafluoroethylene (PTFE) filtration membrane, and drying to obtain graphene microspheres; and preparing a graphene microsphere separation medium taking Reactive Blue 4 as a ligand. According to the method provided by the invention, the affinity separation medium is used for separating and purifying a lot of papain from papaya powder, testing the enzymatic activity and the protein content, and calculating purification folds; and the method provided by the invention is quick, simple and convenient, is large in separation quantity, good in activity and high in purity of an extracted enzyme, and is suitable for scale production.

Description

technical field [0001] The invention belongs to the field of preparation of bioseparation materials, in particular to a method for preparing a graphene microsphere bioseparation medium with controllable particle size. Background technique [0002] Papain (EC3.4.22.2) is a kind of sulfhydryl protease, which widely exists in the root, stem, leaf and fruit of papaya (Carica papaya), and is most abundant in immature milk. Widely used in food industry, such as clarification of beer and tenderization of meat, as well as leather, textile, daily chemical and pharmaceutical industries. The purification of papain mostly adopts the precipitation method, but this method is still mixed with other proteases, which cannot meet the needs of the pharmaceutical industry. [0003] At present, the methods for separating and purifying papain include rough separation and refined separation, wherein rough separation includes salting out method, isoelectric point precipitation method, organic solv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281B01J20/30
Inventor 聂华丽舒黎幼
Owner 佛山市富馨科技实业有限公司
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