39results about How to "Avoid the risk of cross-contamination" patented technology

The invention provides a probe based on double-signal amplification triggered by a target and application of the probe. The probe comprises a first hairpin, a second hairpin and a third hairpin, wherein the first hairpin, the second hairpin and the third hairpin are formed by pairing hybridization of complementary bases in folding areas after single-chain linear molecules are folded back; the parts, forming double-chain structures in the partial areas, of the hairpins are stem areas, and the parts, not forming the double-chain structures and folded back, are ring-shaped areas. According to the probe provided by the invention, the self-assembly of hairpins is realized through target protein catalysis, signal amplification is achieved under the assistance of an excision enzyme III, obvious amplification of a detection signal is realized, and the detection sensitivity is greatly improved further; since the probe does not depend on template replication in the detection process, the problem that false positive occurs due to cross contamination in the detection process is avoided, generation of a false positive signal is effectively prevented and background noise is reduced greatly.

The invention relates to the technical field of in-vitro nucleic acid detection and particularly provides a primer group and kit for detecting polymorphic sites of aspirin-resistant related genes, andan application thereof. The primer group and the kit for detecting polymorphic sites of aspirin-resistant related genes and the kit can detect polymorphism of genes such as COX1, COX2, GPIIIa, PEAR1,P2Y1, GPIa and PAI-1; the sensitivity is high, so that genome DNA low to 0.1ng / muL can be accurately detected; the specificity is good, the genome DNA up to 300ng / muL can not generate non-specific amplification; the whole Fluorescence PCR detection process can be finished only within 90 minutes, and the detection result is intuitive and easy in interpretation.

The invention relates to the technical field of biological pharmacy, in particular to a culture assembly and a medicine screening biological reaction device. The culture assembly comprises a culture plate; a plurality of groups of micro-flow culture chambers are arranged on the culture plate; each group of micro-flow culture chambers comprises a plurality of pore chambers; a fluid communication channel is arranged between every two adjacent pore chambers; each fluid communication channel is provided with a circulation valve; each pore chamber is internally provided with a three-dimensional bracket matched with the pore chamber correspondingly; a fluid passing cavity is formed between the bottom of each three-dimensional bracket and the bottom of the corresponding pore chamber; a supporting bracket layer is arranged at the bottom of the three-dimensional bracket; the supporting bracket layer comprises a plurality of through holes, and an electrostatic spinning film is arranged on the supporting bracket layer; and a liquid inlet and a liquid outlet which are communicated with the fluid communication channel are formed in each group of micro-flow culture chambers correspondingly. According to the culture assembly, each micro-flow culture chamber channel is separated, the risk of cross contamination is avoided, and the form of the flow valve is adopted, so that liquid flows in an S-shaped wave form, and local stagnant water is avoided.

The invention provides an MTHFR and MTRR gene polymorphism detection primer and probe combination, which includes an MTHFR C677T, MTHFR A1298C and MTRR A66G gene polymorphism detection primer and probe combination. The invention further provides a detection kit for the MTHFR and MTRR gene polymorphism. The kit is prepared from the following components of a nucleic acid releasing agent, 2*PCR reaction mixture (including a thermally activated Taq DNA polymerase and a UDG enzyme), three sets of specific primers, three sets of specific probes and an internal standard system. The kit only needs tobriefly treat a 2 [mu]l blood sample at the room temperature to produce high-quality DNA for repeated detection, the detection specificity is good, the coincidence rate with a gold standard sequencingmethod is 100%, the sensitivity is high, accurate typing of 0.2ng genomic DNA can be achieved, the detection process is short in consumed time, the process from sample processing to detection resultscan be completed within 1 hour.

The invention relates to the technical field of in-vitro diagnosis, and in particular relates to a quantitative detection method for gamma-interferon and a kit. The quantitative detection method for the gamma-interferon disclosed by the invention comprises the following steps: (1) preparing a fluorescent trapping microsphere by adopting a carbodiimide method; (2) releasing the gamma-interferon in vitro; (3) combining the fluorescent trapping microsphere with the gamma-interferon; (4) preparing an antigen-antibody complex; and (5) quantitatively detecting. The quantitative detection kit for gamma-interferon disclosed by the invention comprises the fluorescent microsphere, a coupling buffer solution, carbodiimide, an NHS activating agent and a fluorescein labelled anti-human gamma-interferon antibody. The quantitative detection method for the gamma-interferon and the kit, disclosed by the invention, are simpler to operate; and simultaneously, quantitative detection of the gamma-interferon is realized effectively.

The invention is applicable to the technical field of lithium ion batteries, and provides a manufacturing method of a bipolar polymer lithium ion battery and a lithium ion battery. A bipolar pole piece, a single-side positive pole piece and a single-side negative pole piece are manufactured by utilizing a positive active material piece, a current collector and a negative active material piece through hot-pressing compounding and a rolling method, the obtained pole pieces adsorb a mixed solution containing an electrolyte solution, and the pole pieces are wrapped by a polymer film through ultraviolet irradiation; and rolling, punching and laminating are performed to obtain a pole group, and rolling, packaging and forming are carried out on the pole group to obtain the bipolar polymer lithiumion battery. The lithium ion battery manufactured by the manufacturing method provided by the invention has the advantages of high safety, small internal resistance, high charging and discharging power, wide working temperature range and long cycle life, and after the lithium ion battery is manufactured into a module and a system, a thermal management device can be simplified, and the overall system manufacturing cost is reduced.

The invention discloses a livestock and poultry farm vehicle intelligent decontamination system and an implementation method, and relates to the field of livestock and poultry breeding equipment. Thesystem comprises a cleaning unit, a draining and disinfecting unit, a drying unit, a personnel disinfecting unit, an article disinfecting unit, a wastewater collecting (treating) unit, a personnel resting unit, a vehicle transferring device, a license plate recognition camera, a livestock and poultry excrement recognition camera, a cleaning effect detecting device, a disinfecting effect detectingdevice and a control system. A vehicle passes through the cleaning unit, the draining and disinfecting unit and the drying unit in sequence to be decontaminated after being detected to be qualified, and the vehicle can enter the next step to be decontaminated after being detected to be qualified through cleaning and disinfecting effects. Personnel and articles are respectively decontaminated by the personnel and article disinfection unit, and the articles can be taken and used after the disinfection effect detection is qualified. Decontamination paths of vehicles, personnel and articles are independent of one another, and the decontamination operation can be synchronously carried out. The decontamination system is reasonable and compact in layout, standard in process, and efficient and intelligent in operation, and the decontamination effect is guaranteed.

The invention discloses a fully-closed pollution-free digital nucleic acid detection chip which comprises a chip body, a first puncture needle and a second puncture needle, the chip body is made of a transparent material, a plurality of oil inlet through holes are formed in the chip body, outlets of the oil inlet through holes are connected with an oil inlet groove, one end, connected with the first puncture needle, of the oil inlet groove is provided with an opening, an opening in the other end of the first puncture needle extends into a test tube, the test tube is used for receiving an oil phase conveyed by the oil inlet through holes, an opening in one end of the second puncture needle is connected into a liquid inlet groove formed in an inner cavity of the chip body, an outlet of the liquid inlet groove is connected with a tiling cavity, the bottom plane of the tiling cavity is in the horizontal direction, and an opening in the other end of the second puncture needle extends into the test tube; and the opening in the other end of the first puncture needle extends into the oil phase in the test tube all the time, and when the test tube is filled with the oil phase, the opening in the other end of the second puncture needle is in contact with the top liquid level of the oil phase. The risk of cross contamination is avoided, operation is easy, and consumed time is short.

The invention provides a method for extracting and purifying pravastatin sodium from a fermentation liquor, and belongs to the field of biological medicine preparation. According to the method for extracting and purifying pravastatin sodium from the fermentation liquor, a large amount of impurities and pigments are removed by alkalizing and acidifying the fermentation liquor, and the effective components in an acidizing liquid are enriched by an adsorbent, so that the purity of an extraction substrate is improved, the quantity of the extraction substrate is greatly reduced, and the quality ofan extraction liquid is further improved; in the whole production process, a single solvent system is adopted, the process is not complicated, the generated three wastes are low in amount, and the method is suitable for large-scale production. The content of pravastatin sodium produced by the method is more than 98.8 percent, the content of main impurity 6-epipravastatin is less than 0.15%, the content of total related substances is less than 0.4%, the product quality accords with the EP9.6 edition quality standard (the content of the pravastatin sodium is between 97.0% and 102.0%; a main impurity A, namely6-epipravastatin, is less than or equal to 0.3%; the content of the total related substances is less than or equal to 0.6%) of qualified pravastatin sodium, the total yield can reach more than 75%, and the large-scale production prospect is good.

The invention relates to the field of medical equipment and especially relates to a disposable anti-pollution and anti-leakage syringe, and the syringe comprises a needle cylinder, a piston matching with the needle cylinder, and a push rod connected with the piston, wherein the syringe further comprises a sealing sleeve which is sleeved on the push rod, one end of the sealing sleeve is in closed connection with that of the needle cylinder opening, and the other end of the sealing sleeve is in closed connection with the piston or the push rod. In comparison with the traditional syringe, the syringe can effectively avoid germs from outside air or hands of medical personnel to enter inside solution so as to further prevent from bacterial infection and cross infection.

The invention provides wafer sorting equipment and a wafer sorting method. The equipment comprises a sorting chamber, wherein the sorting chamber comprises a first layer chamber and a second layer chamber, a first discharging port is formed in the discharging end of the first layer chamber, and a first conveying belt is arranged in the first layer chamber, a second discharging port is formed in the discharging end of the second layer chamber, and a second conveying belt is arranged in the second layer chamber, a filtering fan unit is arranged above the sorting chamber; and a calibrator unit, wherein the calibrator unit is located at the feeding end of the sorting chamber, the calibrator unit is at least provided with a first feeding port and a second feeding port, at least two mechanical arms are arranged in the calibrator unit, a first mechanical arm is used for transferring OK wafers from the first feeding port to the first conveying belt of the first layer chamber, and a second mechanical arm is used for transferring NG wafers from the second feeding port to the second conveying belt of the second layer chamber. According to the wafer sorting equipment and the wafer sorting method, the environment cleanliness during wafer sorting can be improved, the pollution risk is reduced, and the efficiency is improved.

The invention discloses a PCR reaction kit for detecting BRCA gene mutation. The PCR reaction kit comprises an integrally installed detection rod, the detection rod comprises a front sleeve, an intermediate tube movably installed at the inner side of the front sleeve, and a tail coil tube screwed at the rear end of the intermediate tube; the front sleeve comprises a reaction detection pipe sectionwith a closed front section and a hollow rear end, and a guide pipe section with two hollow ends; a sample injection funnel is fixed at the open end of the reaction detection pipe section; an ejectorpin is fixed on the inner side of the sample injection funnel, and a wax seal is arranged at the bottom of the sample injection funnel; the reaction detection pipe section is communicated with the guide pipe section through a soft sleeve. According to the PCR reaction kit for detecting BRCA gene mutation, the integrated detection rod is adopted, full-closed tube operation is adopted in the amplification process, the risk of cross contamination can be greatly avoided, and therefore the accuracy of BRCA gene mutation detection is guaranteed; in addition, the whole amplification process is simple and convenient, and the amplification operation efficiency is high.

The invention discloses a cap screwing method. According to the technical key points, the cap screwing method comprises the following steps that S1, a pipe cap is clamped, specifically, a clamping jaw motor is started, so that the pipe cap is clamped by clamping jaws; and S2, the pipe cap is screwed, specifically, after a rotating motor is started, a driving wheel is driven to rotate forwards or reversely, the driving wheel drives synchronous wheels to rotate forwards or reversely through a synchronous belt, the four synchronous wheels correspond to the four clamping jaws correspondingly, and forward rotation or reverse rotation of the synchronous wheels drives the four clamping jaws to rotate forwards or reversely, so that full-automatic unscrewing / screwing of a sampling pipe cap is realized. By the adoption of the cap screwing method, four pipe caps can be screwed / unscrewed synchronously.

The invention discloses a sample processing and detecting multifunctional device. The sample processing and detecting multifunctional device comprises a box body, an extraction mechanism, a liquid suction mechanism and a transmission mechanism, wherein a plurality of treatment chambers which are isolated from one another and a sample adding channel for adding samples are arranged in the box body;the extracting mechanism comprises an extracting guide rail and an extracting part, the extracting guide rail is arranged on the inner side wall of the box body, a part of the extracting guide rail isof an up-down circuitous structure, and the extracting part is movably connected to the extracting guide rail and further connected with the transmission mechanism; the liquid suction mechanism comprises a liquid suction guide rail and a liquid suction part, the liquid suction guide rail is arranged on the inner side wall of the box body, a part of the liquid suction guide rail is of an up-and-down circuitous structure, the liquid suction part is movably connected to the liquid suction guide rail, the liquid suction part is further connected with the transmission mechanism, and the liquid suction part is further communicated with the sample adding channel. The sample processing and detecting multifunctional device can avoid the risk of sample infection and cross contamination in the detection process.

The invention relates to the field of laboratory containers, in particular to a test tube capable of transferring a liquid at the upper end and a test tube assembly. The test tube comprises a tube body, wherein the lower end of the tube body is a closed tube bottom, a tube opening is formed in the upper end of the tube body, a wall bulge protruding towards the radial outer side of the tube body isarranged below the tube opening of the upper part of the tube body, and the wall bulge is provided with a concave cavity for allowing the liquid in the test tube to flow into the test tube when beingoverturned to a low position. The test tube assembly comprises the test tube capable of transferring the liquid at the upper end and a flip type tube cap which covers the upper end including the wallbulge of the test tube. According to the scheme, the problems of inconvenience caused for the reason that when the existing test tube is used, a pipette or a suction head needs to be inserted into the tube bottom to transfer the liquid and cross contamination which is easily caused for the reason that a liquid-transferring machine rod is inserted into the tube body can be solved, and the flip type test tube assembly can avoid the risk of falling and mismatch of a split type tube cap, and is convenient to operate.

The invention provides an intelligent tableware disinfecting cabinet. The disinfecting cabinet consists of an upper cover, a cabinet body and an intelligent control system; the upper cover includes aautomatic human body inductor, a flip cover and a flip cover driving mechanism; the cabinet body includes an ozone generator, electric heating pipes and a tableware storage and elevating mechanism; and the intelligent control system includes temperature control, ozone concentration control, elevating mechanism start-stop control, elevating mechanism motion directional control and flip cover open-close control. When the automatic human body inductor inducts that someone takes tableware, the flip cover can automatically opened and closed after the tableware are taken away; when the tableware reserved at a tableware taking position is used up, the elevating mechanism can automatically push out the tableware from the cabinet body to supplement; and when all the tableware in the cabinet body are used up, the addition of the tableware can be prompted by a working status indicator, and a tableware position sensor can control the elevating mechanism to cooperate with an operator to add the tableware in a gradual declining manner. The disinfecting cabinet is mainly used for the sterilization, disinfection and storage of the tableware, is suitable for various tableware such as plates, bowlsand trays and has the advantages of being convenient in tableware taking and simple in equipment operation; and the problems of hand cross contamination during the opening of a door can be avoided when people take the disinfected tableware, so that the disinfecting cabinet is especially suitable for public dining places such as buffet restaurants, fast food chain stores, hotels and unit canteens.

The invention discloses a negative-pressure weighing device. The negative-pressure weighing device comprises a weighing box body; a weighing chamber is defined at the front part of the interior of theweighing box body; the bottom of the weighing chamber is provided with a weighing device; the rear part of the weighing chamber is provided with a back plate which plays a role of separation; the topof the weighing chamber is provided with a static pressure box; a filtering chamber is formed behind the back plate; the bottom of the back plate is provided with a filtering screen; the bottom of the filtering chamber is provided with a primary effect filter; the primary effect filter is corresponding to the filtering screen; the rear of the primary effect filter is provided with an intermediateeffect filter; the top surface of the intermediate effect filter is provided with a circulation fan; the circulation fan is connected with the static pressure box through an air pipe; the front sidesurface of the static pressure box is provided with a side air exhausting high-efficiency filter; the side air exhausting high-efficiency filter is provided with a regulating valve; and the bottom surface of the static pressure box is provided with a unidirectional flow high-efficiency filter. With the negative-pressure weighing device of the invention adopted, a dust removal requirement can be satisfied; dust overflow and rise can be controlled; the risk of dust cross-contamination can be avoided; the dust can be prevented from being inhaled by a human body and hurting the human body; the safety of an external environment and people in an indoor environment can be protected.

The present disclosure provides a wafer sorting equipment and a wafer sorting method, the equipment includes: a sorting chamber, including a first layer chamber and a second layer chamber, the feeding end of the first layer chamber is provided with The first feeding port, the first conveyor belt is arranged in the first floor chamber; the second feeding port is provided at the feeding end of the second layer chamber, and the second conveyor belt is arranged in the second layer chamber; A filter fan unit is arranged on the top of the filter; the calibrator unit is located at the feeding end of the sorting chamber, the calibrator unit is provided with at least a first feeding port and a second feeding port, and at least two mechanical arms are arranged inside the calibrator unit , the first robotic arm is used to transfer OK wafers from the first loading port to the first conveyor belt of the first chamber, and the second robotic arm is used to transfer NG wafers from the second loading port to the second on the second conveyor in the layer chamber. The wafer sorting equipment and the wafer sorting method provided in the present disclosure can improve environmental cleanliness during wafer sorting, reduce pollution risks, and improve efficiency.

A D-dimer tester comprises a rotary sample application arm, a reagent position, a test position, a double tube pump, a liquid collection tube, a sample position, a microinjection pump and a needle washing position, wherein the sample position, the reagent position, the test position and the needle washing position are in a same working platform. The D-dimer tester provided by the invention is easy to operate, avoids the risk of cross contamination, has high consistency of test results, stable test and high precision, and meets market demands; and the unique alarm function of the D-dimer tester can avoid errors in manual operation, so as to ensure the correctness of test results in a hospital.

The invention relates to the technical field of medical instruments, and particularly discloses a soaking and disinfection device for a rigid endoscope. The device comprises a shell, wherein a cleaning barrel is arranged in the shell, a liquid outlet and an inserting opening allowing the rigid endoscope to be inserted are formed in the cleaning barrel, a drainage pipe is connected to the liquid outlet, a drainage valve is arranged on the drainage pipe, and a heating piece is arranged on the cleaning barrel; and a clean water barrel, an enzyme solution barrel and a disinfectant barrel which allcommunicate with the cleaning barrel are arranged in the shell, a first pump body communicates between the clean water barrel and the cleaning barrel, a second pump body communicates between the enzyme solution barrel and the cleaning barrel, and a third pump body communicates between the disinfectant barrel and the cleaning barrel. According to the scheme, the problem that in the prior art, whenthe rigid endoscope is taken out of a rigid endoscope cleaning disinfection machine and stored after cleaning and disinfection, the rigid endoscope is likely to be polluted is solved.

The invention provides a filter device used for a micro device. The filter device is used for filtering a liquid flowing into the micro device. The filter device comprises a filter and a connecting part, wherein the filter is fixed onto the micro device, and comprises an input end and an output end; the connecting part is used for connecting the output end of the filter and the first end of the micro device; the liquid flows into the filter from the input end of the filter, flows out from the output end of the filter, and then flows into the micro device after flowing through the connecting part. After the technical scheme is adopted, integrated integration of the filter device and the micro device is realized, and the filter device is beneficial for volume production; the filter device isconvenient to install and maintain, the service life of the micro device is prolonged, and the cost is reduced; the filter device does not exceed the original size of the micro device, and is convenient to use in narrow working environments; the cross contamination risk of a large device in the prior art is avoided.

The invention discloses a general electrode coating system for lithium / sodium ion batteries, which comprises a support plate, a base plate, a brushing device and a linear movement mechanism, a base is arranged on the support plate, a slot is recessed on the side of the base plate, and a slot is provided on the slot There is a buffer device inside, and when the support plate is inserted into the slot, it has a compressive force on the buffer device; a pressing device is provided outside the slot, and the pressing device is used to limit the ejection of the support plate; the brushing device includes a The upper roller has a roller shaft inside the roller, which can rotate around the roller shaft; the two ends of the roller shaft extend to the outside of the roller and are fixedly connected with the lifting structure respectively; the linear movement mechanism includes a screw rod, which is arranged on the bottom plate Sliders are slidably arranged on the two sides of the screw rod, and the sliders can pull the brushing device to slide through the connecting member. The invention prevents the displacement of the support plate and the battery base during the coating process through the buffer device and the pressing device, thereby ensuring the stability of the coating process.

The invention belongs to the field of nuclear fuel after-treatment technology and particularly relates to a mechanical arm separation module for an automatic separation device of nuclear samples. Themechanical arm separation module comprises a reagent storage groove bracket, a separation column bracket, a waste liquid treatment assembly, a slide rail, a mechanical arm platform, a sample bottle bracket and a lifting plate. The mechanical arm separation module is provided with five moving shafts, including three shafts for helping a sampling probe to complete movements in X-axis, Y-axis and Z-axis directions, the lifting plate for enabling the sample bottle bracket to move in the Z-axis direction, and the slide rail for enabling the separation column bracket to move in the X-axis direction,and complicated separation processes can be completed by perfect cooperation of the five shafts, so that the used space of the inside of a glove box is reduced, repair and maintenance are facilitated, the flowing time of reagents and samples is shortened, the liquid sampling accuracy is improved, the separation time is shortened, the efficiency is improved, and the function of performing sample loading separation twice after dilution is performed on eluent once is realized.

The invention relates to a liquid level detecting device for an active immunity workstation and belongs to the technical field of medical examination, food examination and biological experimental facilities. The liquid level detecting device is characterized in that a plunger pump is arranged on a sampling arm cross bar; the plunger pump is fixed with the sampling arm cross bar; a solenoid valve is connected with a connecting end of a plunger pump chamber and is fixed on the plunger pump through a bolt; a sampling head connecting mechanism is vertically fixed on the sampling arm cross bar; thesampling head connecting mechanism is connected with the plunger pump through a hose; a pressure sensor in the connecting pipeline is connected with the pipeline. The liquid level detecting device has the beneficial effects that: 1) the risk of cross contamination is avoided, 2) the material cost of instrument is reduced, 3) the plunger pump is directly fixed on the sampling arm cross bar so thatthe length of the connecting pipe of the sampling head connecting mechanism is shortened and the sampling accuracy is increased, and 4) the solenoid valve is used for controlling the connection of the pipeline and air, the pipeline pressure directly recovers to the initial air pressure after the plunger pump is started, and the pressure change in the pipeline caused by the resetting of the plunger pump can be avoided.

The invention discloses a sampling and liquid level detection device for an automatic sampling system, which belongs to the technical field of medical, food testing and biological experimental equipment. The device is characterized in that: an elastic board is arranged on a sampling arm beam; the inner end of the elastic board is fixed with the sampling arm beam; a sampling nozzle connecting mechanism vertically runs through the sampling arm beam to be fixed with the outer end of the elastic board; a sampling nozzle in-place detection sensor is fixed at the outer end of the sampling arm beam;a sampling nozzle in-place separation blade is arranged on the sampling nozzle in-place detection sensor and the outer end of the elastic board; a negative pressure generation mechanism and a plungerpump are in closed communication with the sampling nozzle connecting mechanism by a three way valve and a pressure difference sensor; and the sampling nozzle connecting mechanism is a conical pipe body. The device has the advantages of 1, avoiding the risk of cross contamination caused by spur sampling adopted by most of conventional apparatuses; and 2, avoiding the high cost of one-off conductive plastic sampling nozzles mostly independent on import at present by adopting ordinary sampling nozzles so as to reduce the apparatus cost.

The invention discloses a multi-cavity chemical vapor deposition device which comprises a box body, a gas supply structure is arranged on the box body, the interior of the box body is divided into a plurality of cavities through a plurality of separation structures, a plurality of heating structures are arranged in the box body, a plurality of lifting structures are arranged in the box body, and the lifting structures are arranged in the box body. The device has the beneficial effects that under the condition that the deposition time is long, such as a diamond film and an amorphous silicon film, but not limited to the diamond film and the amorphous silicon film, deposition can be carried out in equipment with three, ten or more cavities at the same time, industrial preparation of film materials is completed in a one-time large-scale mode, and the device is suitable for large-scale production. The hardware investment cost and the operation cost are saved; the deposition process can be carried out in the single cavity according to the process conditions under the complex process conditions and sequence, and the distance between the substrate and the hot wire and the distance between the substrate and the gas distribution system are adjusted through the lifting platform, so that the influence on the product quality caused by side reaction is avoided.

The invention discloses a sampling device for an automatic immunization system and a sampling method. The sampling device comprises a sampling arm. The sampling arm comprises a crossbeam with one end connected with an output terminal of an output shaft and the other end provided with a cylindrical sampling head coupling mechanism. The sampling head coupling mechanism includes a middle cavity chamber penetrating from top to bottom; an opening at top of the sampling head coupling mechanism is connected with a connection part of a three-dimensional communication valve; the other two connection parts of the three-dimensional communication valve are respectively connected with a connection part of a negative pressure generating mechanism and a connection part of a plunger pump through corresponding fluid path pipes; a differential pressure sensor is arranged on the fluid path pipe between the three-dimensional communication valve and the sampling head coupling mechanism. The sampling method comprises a sample liquid absorption process based on the above sampling device. The invention can realize a function of automatic detection on a sample liquid level and liquid absorption sampling by the sampling head, has high sampling precision; besides usage of a disposable TIP head reduces cost and avoids cross contamination.