PCR reaction kit for detecting BRCA gene mutation

Abstract

The invention discloses a PCR reaction kit for detecting BRCA gene mutation. The PCR reaction kit comprises an integrally installed detection rod, the detection rod comprises a front sleeve, an intermediate tube movably installed at the inner side of the front sleeve, and a tail coil tube screwed at the rear end of the intermediate tube; the front sleeve comprises a reaction detection pipe sectionwith a closed front section and a hollow rear end, and a guide pipe section with two hollow ends; a sample injection funnel is fixed at the open end of the reaction detection pipe section; an ejectorpin is fixed on the inner side of the sample injection funnel, and a wax seal is arranged at the bottom of the sample injection funnel; the reaction detection pipe section is communicated with the guide pipe section through a soft sleeve. According to the PCR reaction kit for detecting BRCA gene mutation, the integrated detection rod is adopted, full-closed tube operation is adopted in the amplification process, the risk of cross contamination can be greatly avoided, and therefore the accuracy of BRCA gene mutation detection is guaranteed; in addition, the whole amplification process is simple and convenient, and the amplification operation efficiency is high.

Description

technical field [0001] The invention relates to a reaction kit, in particular to a PCR reaction kit for detecting BRCA gene mutation, and belongs to the technical field of reaction kits. Background technique [0002] BRCA is a gene that can inhibit the occurrence of malignant tumors. It plays an important role in regulating the replication of human cells, the repair of genetic material DNA damage, and the normal growth of cells. There are hundreds of BRCA mutations that have been found, some of which are related to hereditary breast cancer. Cancer and ovarian cancer are related, summarizing the lifetime risk of cancer related to BRCA gene mutation shows that the risk of breast and ovarian cancer in people with BRCA gene mutation is 50%-85% and 15%-45%, respectively, compared with ordinary women , These are very high chances of getting cancer. Since the mutation of BRCA gene has a certain relationship with the occurrence of familial breast cancer and ovarian cancer, the detec...

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Problems solved by technology

[0002] BRCA is a gene that can inhibit the occurrence of malignant tumors. It plays an important role in regulating the replication of human cells, the repair of genetic material DNA damage, and the normal growth of cells. There are hundreds of BRCA mutations that have been found, some of which are related to hereditary breast cancer. Cancer and ovarian cancer are related, summarizing the lifetime risk of cancer related to BRCA gene mutation shows that the risk of breast cancer and ovarian cancer in people with BRCA gene mutation is 50%-85% and 15%-45%, respectively, compared with ordinary women , These are very high chances of getting cancer. Since the mutation of BRCA gene has a certain relationship with the occurrence of familial breast cancer and ovarian cancer, the detection of BRCA mutation gene has important clinical significance for the diagnosis, prevention and treatment of related cancers. Significance, in the prior art, BRCA gene mutation detection method is mainly restriction small fragment length polymorphism analysis (RFLP method) and DNA direct sequencing method, and RFLP method is the method that PCR is combined with restriction enzyme digestion; Current The detection reagent is easily infected by external bacteria, and it is difficult to avoid cross-contamination due to non-closed tube operation

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