Culture assembly and medicine screening biological reaction device

Abstract

The invention relates to the technical field of biological pharmacy, in particular to a culture assembly and a medicine screening biological reaction device. The culture assembly comprises a culture plate; a plurality of groups of micro-flow culture chambers are arranged on the culture plate; each group of micro-flow culture chambers comprises a plurality of pore chambers; a fluid communication channel is arranged between every two adjacent pore chambers; each fluid communication channel is provided with a circulation valve; each pore chamber is internally provided with a three-dimensional bracket matched with the pore chamber correspondingly; a fluid passing cavity is formed between the bottom of each three-dimensional bracket and the bottom of the corresponding pore chamber; a supporting bracket layer is arranged at the bottom of the three-dimensional bracket; the supporting bracket layer comprises a plurality of through holes, and an electrostatic spinning film is arranged on the supporting bracket layer; and a liquid inlet and a liquid outlet which are communicated with the fluid communication channel are formed in each group of micro-flow culture chambers correspondingly. According to the culture assembly, each micro-flow culture chamber channel is separated, the risk of cross contamination is avoided, and the form of the flow valve is adopted, so that liquid flows in an S-shaped wave form, and local stagnant water is avoided.

Description

technical field [0001] The invention relates to the technical field of drug screening, in particular to a culture component and a drug screening biological reaction device. Background technique [0002] Drug screening is the initial and key step in the development of new drugs, and there are problems of high cost, long cycle, complicated process and low success rate in the development of new drugs, which restrict the screening and development of new drugs. At present, most of the bioreactors used in drug screening are three-dimensional perfusion cell culture. Not only can drug factors and chemical culture solution be continuously added to the reactor to realize accurate screening of various drugs, but also the dynamic culture of three-dimensional cells can avoid the three-dimensional culture. The scale of the scaffold is too large, resulting in uneven mass transfer between the cells in the center of the three-dimensional scaffold and the cells at the edge of the scaffold. H...

AI Technical Summary

Problems solved by technology

For example, the currently used bioreactor reactor chamber and its preparation method mainly have the following deficiencies: (1) The dimensions and specifications of the reactor are designed by different developers, and there is no universality

(2) It is not easy to observe the growth of cells in the reaction chamber and on the fiber carrier

(4) It is generally a closed structure, and it is inconvenient to add auxiliary experimental drugs, etc.

(5) It is not suitable for soft materials such as hydrogel and fragile materials, and cannot carry

(6) The amount of the common culture plate needs to be converted and calculated, and the control experiment cannot be directly carried out

[0004] To sum up, traditional drug screening systems are mostly 2D cultures that cannot simulate the internal environment of 3D cultures in vivo, and single-nozzle 3D printing cannot guarantee the real-time and uniformity of biological cell scaffolds.

On the one hand, it cannot simulate the 3D internal environment in the body well; on the other hand, the inconsistency of biological cell materials affects the accuracy of drug screening results

The accuracy of the drug screening effect of the 2D culture method in the existing patent is unreliable, and the single-nozzle 3D printing drug screening biological reaction device cannot achieve the uniformity and simultaneity of the printed scaffold material, which will affect the drug screening results to a certain extent. Simultaneous single-nozzle printing The operation process is complicated and there is a risk of cross-infection

Therefore, the current drug screening system has many deficiencies in the accurate screening of drugs.

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