A pcr reaction kit for detecting brca gene mutation

Abstract

The invention discloses a PCR reaction kit for detecting BRCA gene mutation, which includes a detection rod installed integrally, the detection rod includes a front sleeve, an intermediate tube movably installed inside the front sleeve, and screwed to the intermediate tube. The tail coil at the rear end; the front casing includes a closed front section, a hollow reaction detection pipe section at the rear end, and a hollow guide pipe section at both ends; a sampling funnel is fixed at the open end of the reaction detection pipe section; the inner side of the sampling funnel is fixed There is a thimble, and the bottom of the sampling funnel is provided with a wax seal; the reaction detection tube section and the guide tube section are connected through a soft sleeve; the PCR reaction kit for detecting BRCA gene mutation of the present invention adopts an integrated detection stick, and the amplification process uses Fully closed tube operation can greatly avoid the risk of cross-contamination, thereby ensuring the accuracy of detecting BRCA gene mutations. In addition, the entire amplification process is simple and convenient, and the amplification operation efficiency is high.

Description

technical field [0001] The invention relates to a reaction kit, in particular to a PCR reaction kit for detecting BRCA gene mutation, and belongs to the technical field of reaction kits. Background technique [0002] BRCA is a gene that can inhibit the occurrence of malignant tumors. It plays an important role in regulating the replication of human cells, the repair of genetic material DNA damage, and the normal growth of cells. There are hundreds of BRCA mutations that have been found, some of which are related to hereditary breast cancer. Cancer and ovarian cancer are related, summarizing the lifetime risk of cancer related to BRCA gene mutation shows that the risk of breast and ovarian cancer in people with BRCA gene mutation is 50%-85% and 15%-45%, respectively, compared with ordinary women , These are very high chances of getting cancer. Since the mutation of BRCA gene has a certain relationship with the occurrence of familial breast cancer and ovarian cancer, the detec...

AI Technical Summary

Problems solved by technology

[0002] BRCA is a gene that can inhibit the occurrence of malignant tumors. It plays an important role in regulating the replication of human cells, the repair of genetic material DNA damage, and the normal growth of cells. There are hundreds of BRCA mutations that have been found, some of which are related to hereditary breast cancer. Cancer and ovarian cancer are related, summarizing the lifetime risk of cancer related to BRCA gene mutation shows that the risk of breast cancer and ovarian cancer in people with BRCA gene mutation is 50%-85% and 15%-45%, respectively, compared with ordinary women , These are very high chances of getting cancer. Since the mutation of BRCA gene has a certain relationship with the occurrence of familial breast cancer and ovarian cancer, the detection of BRCA mutation gene has important clinical significance for the diagnosis, prevention and treatment of related cancers. Significance, in the prior art, BRCA gene mutation detection method is mainly restriction small fragment length polymorphism analysis (RFLP method) and DNA direct sequencing method, and RFLP method is the method that PCR is combined with restriction enzyme digestion; Current The detection reagent is easily infected by external bacteria, and it is difficult to avoid cross-contamination due to non-closed tube operation

Images