A kind of extraction method of biological fermentation carotenoid
A carotene and extraction method technology, applied in chemical instruments and methods, hydrocarbons, extraction purification/separation, etc., can solve the problems of increasing oxidation risk, increasing the difficulty of carotenoid extraction process, reducing purity, etc.
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Embodiment 1
[0084] A method for extracting biologically fermented carotenoids, using Saccharomyces cerevisiae fermented liquid expressing lycopene as a raw material for extraction, specifically:
[0085] (1) Spray-dry the 20L lycopene fermented liquid after fermentation and putting it into the tank, set the inlet air temperature to 190°C, and the outlet air temperature to 90°C, spray dry to obtain 1kg of dry yeast powder;
[0086] (2) The dry yeast powder is humidified with purified water and adjusted to a water content of 10%, and then the wall is broken by a low-temperature shearing machine for 30 minutes. The cooling form is closed circulating water cooling, and the temperature is 8°C. The wall-broken bacteria powder is collected, and the wall-breaking rate is greater than 98% after testing, and the loss of lycopene content is less than 2%;
[0087] (3) Add 10 L of ethanol solution with a sodium hydroxide content of 1% to the dried bacterial powder after the wall breaking for saponific...
Embodiment 2
[0093] A method for extracting biologically fermented carotenoids, using Saccharomyces cerevisiae fermented liquid expressing β-carotene as a raw material for extraction, specifically:
[0094] (1) Spray-dry the 22L beta-carotene fermented liquid after fermentation and putting into the tank, set the air inlet temperature to 180°C, and the air outlet temperature to 80°C, spray dry to obtain 1.32kg of dry yeast powder;
[0095](2) The dry yeast powder is humidified with purified water and adjusted to a water content of 10%, and then the wall is broken by a low-temperature shearing breaker for 45 minutes. The cooling form is closed circulating water cooling, and the temperature is 4°C. Collect the broken wall bacteria powder, the broken wall rate is more than 99% after testing, and the loss of β-carotene content is less than 1.5%;
[0096] (3) Add 6.6L of ethanol solution with a sodium hydroxide content of 2% to the dried bacteria powder after the wall breaking for saponification...
Embodiment 3
[0102] A method for extracting biologically fermented carotenoids, using astaxanthin-expressing Saccharomyces cerevisiae fermentation broth as raw material for extraction, specifically:
[0103] (1) Spray-dry the 25L astaxanthin fermented liquid after fermentation and putting it into the tank, set the air inlet temperature to 190°C, and the air outlet temperature to 90°C, and spray dry to obtain 1.4kg of dry yeast powder;
[0104] (2) The dry yeast powder is humidified with purified water and adjusted to a water content of 9%, and then the wall is broken by a low-temperature shearing breaker for 60 minutes. The cooling form is closed circulating water cooling, and the temperature is 0°C. The wall-broken bacteria powder is collected, and the wall-breaking rate is greater than 99% after testing, and the loss of astaxanthin content is less than 2.5%;
[0105] (3) Add 8.4 L of ethanol solution with a sodium hydroxide content of 3% to the dried bacterial powder after the wall break...
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