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Aspergillus fungus with nematode-killing vitality, and its preparing method and use

A technology for killing nematodes and fungi, which is applied in the field of fungi that poison and kill plant parasitic nematodes and the preparation thereof, can solve the problems of polluted environment, poor selectivity, large waste of chemical nematicides and the like

Inactive Publication Date: 2007-01-10
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has poor selectivity, strong extinction, and damages soil biota. Zhang Keqin, He Shichuan, Zhou Wei, etc. 1991. The role and research progress of fungi and bacteria in nematode biocontrol. Insecticidal Microbes. 3: 55-66), the 21st century is called the environmental protection century, some effective chemical nematicides Therefore, the research on the biological control of plant parasitic nematodes has naturally become a hot issue.

Method used

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  • Aspergillus fungus with nematode-killing vitality, and its preparing method and use
  • Aspergillus fungus with nematode-killing vitality, and its preparing method and use
  • Aspergillus fungus with nematode-killing vitality, and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Cultivation of bacterial strain A. niger snf0407009

[0019] The mycelium of A. niger snf0407009 was inoculated on the test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; water 1000mL. Incubate at 25°C for 10 days to obtain test tube seeds.

[0020] Inoculate the test tube into 250mL Erlenmeyer flask (50mL per bottle) liquid medium again, the medium formula is (percentage by weight): sucrose (2.83%), ammonium sulfate (1.367%), K 2 HPO 4 (0.064%), MgSO 4 ·7H 2 O (0.182%), KCl (0.996%), FeSO 4 (0.02%), the rest is water, pH 6.5. At 25°C to 28°C, the shaker speed is 150r·min -1 ~200r·min -1 , fermentation 240h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.

Embodiment 2

[0021] Example 2: Cultivation of strain A. niger snf0407009

[0022] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0023] Liquid medium formula is: sucrose (3%), ammonium sulfate (5%), K 2 HPO 4 (0.08%), MgSO 4 ·7H 2 O(0.4%), KCl(2.0%), FeSO 4 (0.002%), pH 7.

Embodiment 3

[0024] Embodiment 3: the culture of bacterial strain A.niger snf0407009

[0025] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0026] Liquid medium formula is: sucrose (6%), ammonium sulfate (3%), K 2 HPO 4 (2%), MgSO 4 ·7H 2 O(0.5%), KCl(5.2%), FeSO 4 (0.04%), pH 6.

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Abstract

The invention relates to a cultivation of epiphyte that has plant infestation wireworm poisoning function and the metabolite manufacture method and the application. The strain of the epiphyte is Asipergillus. niger snf0407009 and the conserving code is CGMCC No.1714. It is made up from normal liquid fermenting and cultivating, and its metabolite has high poisoning function to plant infestation wireworm, especially root wireworm. The influence to the Meloidogyne incognita has been measured indoor. Different thickness fermenting liquid would have obviously influence to Meloidogyne incognita. One time fermenting liquid has the similar function as 10ug / mL aldicarb. It has good application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00
Inventor 段玉玺朱晓峰陈立杰
Owner SHENYANG AGRI UNIV
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