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Method for detoxification tissue culture and rapid propagation of dianthus caryophyllus

A technology of tissue culture rapid propagation and carnation, applied in the field of plant tissue culture, can solve the problems affecting germplasm quality and breeding, regenerated plants infected with viruses or fungi, etc., and achieve the effect of avoiding the influence of uncertain factors and simple operation and process

Inactive Publication Date: 2014-03-19
广州市绿洲园艺花卉有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carnations usually use cuttings or tissue culture for large-scale artificial breeding. Cutting plants are easier to survive, but they need a large number of original cuttings to carry out; tissue culture technology can breed a large number of microtraditions in a short period of time, but the tissue culture process It is common for callus and regenerated plants to be infected with viruses or fungi, which affects the quality and breeding of germplasm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for detoxification tissue culture and rapid propagation of carnation, comprising the steps of:

[0017] (1) Heat treatment: Carnation plants were heat-treated at 36-38°C for one week, the light intensity was 3000-4000Lx, and the photoperiod was 12h;

[0018] (2) Disinfection of the surface of the explants: use the lateral buds grown between the leaf axils of Carnation plants as the explants, wash them with tap water for 30 minutes, then disinfect them with 75% ethanol for 30 seconds, and then treat them with 4% sodium hypochlorite for 7 minutes. Finally, wash 5 times with sterile distilled water, and absorb excess water with sterile absorbent paper for later use;

[0019] (3) Shoot apex stripping: cut off the leaves to expose the bud body, and peel off the young leaves with a sterilized dissecting needle until only the 2 youngest leaf primordia are left;

[0020] (4) Shoot tip culture: Inoculate the leaf primordia with the last 2 young leaves on the induction ...

Embodiment 2

[0022] The method is the same as in Example 1, except that the formulation of the induction medium is that 30 g of sucrose, 7 g of agar, 3 mg of IAA, 1.5 mg of NAA, and 2 mg of ZT are added to 1 L of MS medium; a total of 100 leaf primordia are inoculated, of which 55 are directly Differentiated into regenerated plants, the regeneration rate was 55%, and 10 differentiated into clustered buds.

Embodiment 3

[0024] The method is the same as in Example 1, except that the formulation of the induction medium is that 30 g of sucrose, 7 g of agar, 1.5 mg of IAA, 0.5 mg of NAA, and 1 mg of ZT are added to 1 L of MS medium; a total of 100 leaf primordia are inoculated, of which 67 1 directly differentiated into regenerated plants with a regeneration rate of 67%, and 15 differentiated into clustered buds.

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Abstract

The present invention discloses a method for detoxification tissue culture and rapid propagation of dianthus caryophyllus. The method comprises steps such as a heat treatment, explant surface disinfection, stem apex peeling, stem apex culture and the like, wherein lateral bud growing between the dianthus caryophyllus plant leaf axils is adopted as the explant, the sufficient sterile explants can be obtained through match use of ethanol and sodium hypochlorite, the explant can be subjected to direct differentiation on an induction culture medium to obtain the regeneration plant, and the regeneration rate of the plant achieves 67%, wherein the induction culture medium comprises 1 L of an MS culture medium, 25-35 g of sucrose, 5-8 g of agar, 1-3 mg of IAA, 0.5-1.5 mg of NAA, and 0.5-2 mg of ZT.

Description

technical field [0001] The invention relates to a method for detoxifying carnation tissue culture and rapid propagation, which belongs to the field of plant tissue culture. Background technique [0002] Carnation ( Dianthus caryophyllus ) is carnation, also known as lion head carnation, musk carnation, big flower carnation, Dutch carnation, which is a plant of Caryophyllaceae and genus Carnation. Carnations are mainly distributed in the temperate zone of Europe and Fujian, Hubei and other places in mainland China. They are native to the Mediterranean region. Carnations are the main export species of Kenya and the largest export flower species of Colombia in the Americas. They are found in Japan, South Korea, Malaysia and other countries in Asia A large number of cultivation, in Europe, countries such as Germany, Hungary, Italy, Poland, Spain, Turkey, the United Kingdom and the Netherlands have a large scale of cultivation, and it is one of the most commonly used flowers in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张明
Owner 广州市绿洲园艺花卉有限公司
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