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A culture medium composition adaptable for carnation stalk induced bud and application thereof

A culture medium, carnation technology, applied in the field of plant tissue culture, can solve the problems of unfavorable large-scale industrial production, high concentration of hormones, and high cost of cultivation, etc., achieve remarkable induction effect, simplify the treatment process, and reduce environmental hormone pollution Effect

Inactive Publication Date: 2007-04-25
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a culture medium composition suitable for inducing buds of carnation stems and its application, so as to overcome the need for high-concentration hormones, easy vitrification, and the need for inducing buds in existing carnation stems. The addition of penicillin makes the cultivation cost expensive, easily causes pollution, and is not conducive to the defects of large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This example is the process of inducing buds from carnation stem segment explants, comparing the induction rate of induced buds and the quality of stem segment induced buds, and screening out the best medium formula.

[0040] 1) Obtaining sterile materials

[0041] Test materials: The carnation variety used for the test is: "0420" Mary, provided by the Shanghai Flower Breeding Farm.

[0042] Select a robust plant of the tested variety, cut off a stem section of about 3 cm, soak it in soapy water for 7-8 minutes, and rinse it with running water overnight. Soak the cleaned explant material in 75% alcohol for 30s on an ultra-clean bench, rinse it with sterile water 2-3 times, then disinfect it with 2% NaClO and add 1-2 drops of Tween-20 for 15-20min , Rinse 6-7 times with sterile water. Then cut about 1 cm stem segment to make the final inoculation explant.

[0043] 2) Stem segment explants induce buds

[0044]Gently absorb the water on the surface of the cut stems wit...

Embodiment 2

[0056] In this example, the stem segments of four carnation varieties were inoculated on the induction medium supplemented with 0.05, 0.1, 0.5 and 1.0 mg / L6-BA shown in Table 2, and the applicability of the medium to different varieties was tested.

[0057] 1. Test materials: The four carnation varieties tested are: "0409" Cherry Blossom, "0413" Magnolia, "0418" Poppy and "0420" Mary, provided by Shanghai Flower Breeding Farm.

[0058] 2. Medium:

[0059] As shown in Table 2, MS medium was supplemented with 0.05, 0.1, 0.5 and 1.0 mg / L 6-BA induction medium. The culture conditions and test methods are the same as in Example 1.

[0060] 3. Observe the test phenomenon and analyze the results:

[0061] The statistical test results are shown in Table 3. "Mary" had the highest induction rate and no vitrification phenomenon, "Sakura" and "Poppy" had medium induction rate and slight vitrification phenomenon, and "Magnolia" had the lowest induction rate and the most vitrification ph...

Embodiment 3

[0067] In this example, carnation stems were inoculated on LS medium, and different types and concentrations of auxin were added to compare the proliferation rate and re-propagation quality, and test the applicability of different mediums.

[0068] 1. Test materials: Carnation varieties tested are: "0409" Cherry Blossom, "0418" Poppy and "0420" Mary, provided by Shanghai Flower Breeding Farm.

[0069] 2. Medium:

[0070] As shown in Table 2, LS was used as the base medium, the medium was added with different concentrations of 6-BA or KT hormone, the sucrose concentration was 3.0%, the agar concentration was 0.7%, and the pH value was 5.8.

[0071] 3. Culture condition and test method are the same as embodiment 1.

[0072] 4. Observe the test phenomenon and analyze the results:

[0073] The LS medium supplemented with 0.05-1.0 mg / L of 6-BA is suitable for inducing buds from the stems of the above varieties, and the bud induction rate is 40%-70%.

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Abstract

The invention relates to cultivate medium composite, used for induce germ at bamboo. Wherein, it adds 6-BA at 0.05-1.2mg / L into MS cultivate medium, to effectively induce germ, while the MS added with 6-BA 1mg / L has best effect. The invention points out that growth adjuster 6-BA can accelerate the germ induction of bamboo. The invention also provides an application of said composite to cultivate the bamboo organism.

Description

technical field [0001] The invention belongs to the field of plant tissue culture. Background technique [0002] Carnation (Dianthus Caryophyllus) is also known as carnation and carnation. It is a perennial herbaceous plant of the family Caryophyllaceae, native to southern Europe. It has delicate stems and leaves, rich and elegant flowers, gorgeous and delicate colors, high ornamental value, and a single flower with a long flowering period. It is suitable for making bouquets and flower baskets. It is one of the four famous cut flowers in the world. [0003] Carnation is not easy to reproduce by seeds, and sexual reproduction is prone to hybridization between varieties, which cannot maintain the good quality of the parents. Cuttings are often used for reproduction in production. However, due to the low propagation coefficient and slow speed of cuttings, it is difficult to meet the needs of the market, especially for some newly bred and newly introduced varieties. Due to the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘丽娜魏秀丽李建粤周根余
Owner SHANGHAI NORMAL UNIVERSITY
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