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Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium

A somatic embryo and culture medium technology, applied in the field of special medium, can solve the problems of cumbersome ways to obtain callus and heavy workload, and achieve good proliferation effect

Inactive Publication Date: 2010-11-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the explants used in the above results are not limited by seasons, the way to obtain callus is cumbersome and requires a lot of work.

Method used

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  • Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium
  • Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium
  • Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Test material selection, culture medium design and inoculation culture

[0039] 1. The source and treatment of test materials:

[0040]The test material of the present invention is selected from the flower buds of Carnation, collected from the flower test base of Huazhong Agricultural University, Wuhan City, Hubei Province, and is a conventional commercially available Carnation variety (the in vitro cultured adult plant of Carnation of the present invention is not affected by varieties and its genotype constraints). The flower bud of carnation collected in the field is sterilized by conventional method (referring to: Li Mingjun compiles, plant tissue culture, China Agricultural Press, 1992 edition), the petal and the receptacle of the carnation flower bud after the disinfection are cut off on the ultra-clean workbench, The petals are separated from the receptacle, and the petals with the bases of the petals are cut into small pieces of 4 mm in length. These ...

Embodiment 2

[0056] Example 2 Effect of the size of flower buds on the callus induction rate of carnation

[0057] The size of carnation buds has a very important influence on the callus induction rate. In this experiment, callus induction was carried out by using carnation flower bracts of different sizes as explants. The size of carnation flower bracts is divided into 0.5~1.0cm, 1.0~1.5cm, 1.5~2.0cm. Different sizes of flower bracts have a very important effect on the induction of callus. The results showed that there was no significant difference in callus induction rate between flower buds with a size of 1.0-1.5 cm and 1.5-2.0 cm, but the callus induction rate was highest when the size of flower bracts was 1.0-1.5 cm. Therefore, flower buds with a size of 1.0-1.5 cm were selected to induce callus.

[0058] Table 2 Effects of flower buds of different sizes on somatic embryo induction of carnation callus

[0059] bract size

Embodiment 3

[0060] Example 3 Effects of Different Concentrations of 2,4-D and BA on Callus Induction of Carnation Flower Bracts

[0061] In plant tissue culture, 2,4-D is a widely used auxin growth regulator, and it is recognized as the most effective auxin for initiating cell dedifferentiation, forming callus or organogenesis . The most significant physiological function of cytokinin BA is to promote cell division.

[0062] Mixed use of auxin and cytokinin BA can regulate callus differentiation

[0063] After cultivating the flower bracts of carnation for about 1 week, a small amount of yellow-white callus can be seen in the swelling part of the incision. After about 1 month, two types of callus can be observed, one is white, translucent and more compact , the surface is shiny, and most of this callus can produce somatic embryos in subsequent subcultures. The other is green, loose and vigorously growing or water-soaked or browned. The state of this callus is difficult to adjust. It fa...

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Abstract

The invention belongs to the field of plant tissue culture, relating to a method for regenerating whole plant by in vitro propagation of flower and plant dianthus caryophyllus. The method comprises the following steps: a) inoculating the bud of the sterilized dianthus caryophyllus into an embryonic callus induction culture medium to induce the callus; b) transferring the callus with favourable development in step a) onto the embryonic callus induction culture medium to be proliferated; c) inoculating the callus with favourable development in step b) onto the embryonic callus induction culture medium, differentiating the induced somatic embryo to obtain a mature somatic embryo; and d) transferring the obtained mature somatic embryo onto the somatic embryo seedling culture medium to be regenerated into a whole plant. The explant adopted by the invention is not limited by seasons and can culture tissues and cells annually; in-vitro dianthus caryophyllus can be obtained by continuously differentiating the embryonic callus and has favourable differentiation effect; the embryonic callus of the dianthus caryophyllus can be successively stored for a long time and can keep the capability of being further differentiated into plantlets.

Description

technical field [0001] The invention belongs to the technical field of plant tissue and cell culture, and is related to the rapid propagation of garden plants, and specifically relates to a method for regenerating a complete carnation plant through direct somatic cell generation using carnation buds as explants. Includes special media for accompanying use. Background technique [0002] Carnation (Dianthus caryophyllus L.), also known as carnation, belongs to the family Caryophyllaceae (Dianthus L.). Native to southern Europe, it is a perennial flower and is one of the four major cut flowers in the world. At the same time, it can also be used as a potted plant for viewing and arranging flower beds. It is an indispensable flower for major festivals and major ceremonial occasions. [0003] The research on tissue culture and rapid propagation technology of carnation was carried out earlier, and many research results have been obtained. However, although carnation is relativel...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 包满珠张俊卫程晓岩王文恩刘国锋宁国贵傅小鹏
Owner HUAZHONG AGRI UNIV
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