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Flower specificity promoter, expression case and plant expression carrier thereof

A plant expression vector and promoter technology, applied in the field of carnation CMB2 gene promoter, can solve the problem of few flower-specific promoters and achieve long-lasting effect

Inactive Publication Date: 2008-03-05
NANJING FORESTRY UNIV
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Problems solved by technology

[0006] At present, there are few flower-specific promoters that have been studied and can be used in flower genetic engineering, and most of them have applied for patents

Method used

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  • Flower specificity promoter, expression case and plant expression carrier thereof
  • Flower specificity promoter, expression case and plant expression carrier thereof
  • Flower specificity promoter, expression case and plant expression carrier thereof

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Experimental program
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Embodiment Construction

[0044] 1. Isolation of the 5' upstream sequence of the CMB2 gene by chromosome walking

[0045] The CMB2 gene is a petal-specific expression gene, which can be expressed continuously and stably from the beginning of flowering to full opening, and its transcripts were not detected in vegetative organs. The molecular structure analysis of CMB2 gene showed that it was consistent with the reported plant MADS box gene. Southern blot analysis showed that the CMB2 gene existed as a single copy in the carnation genome [7] .

[0046] The regulation-related sequence of the CMB2 gene in GenBank (Accession number: L40805) has only 268 bp of the upstream sequence of the gene translation start site, and only contains a small amount of cis-acting elements. Therefore, the present invention adopts anchor PCR (APCR) and inverse PCR (IPCR) gene cloning methods to clone 3080 bp upstream regulatory sequence of translation start site of carnation floral organ-specific expression gene CMB2 after t...

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Abstract

The present invention relates to one specific flower promoter obtained through cloning carnation CMB2 gene, and the expression box and plant expression vector of the promoter. The specific flower promoter can drive the high efficiency specific expression of target gene in the flower organ, especially in petal and filament, of transgenic plant without expression in root, stem, leaf and other vegetative organs. The promoter of the present invention may applied in flower gene engineering to cultivate novel beautiful durative strain of carnation and other flowers.

Description

1. Technical field [0001] The present invention relates to genetic engineering of flowers, more precisely, the present invention relates to the promoter of the isolated carnation (Dianthus caryophyllus L.) CMB2 gene, which can drive a polynucleotide sequence or a target gene to be expressed at a high level in the flower organ of the plant , and not expressed in vegetative organs such as roots, stems and leaves (including but not limited to). 2. Background technology [0002] Due to the high degree of heterozygosity of carnations, the more successful lines in modern business are generally asexual reproduction of individuals with better quality to maintain their excellent shape. As an ornamental crop, flower color, flower fragrance, flower shape, and prolonging the vase life are important goals of breeding. Selecting excellent strains and transferring the target gene that can change the traits of flowers into carnations provides a new way for cultivating novel, beautiful and ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82
Inventor 黄敏仁郭素敏高磊徐立安诸葛强陈英戴咏梅蔡有铭
Owner NANJING FORESTRY UNIV
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