35 results about "Aspartic Acid Proteases" patented technology

The present invention relates to a method for modifying polyamide. The method comprises that polyamide is contacted with an enzyme preparation comprising an effective amount of protease enzyme in aqueous environment under conditions suitable for the function of the enzyme. The enzyme is preferably selected from the group of aspartic proteases, cysteine proteases and metallo-proteases.

The invention discloses a preparation method for a pea aspartic acid protease inhibitor, and belongs to the technical field of a plant extraction biological activity product. The method comprises the following steps of: directly grinding pea into powder; leaching with water; performing high-speed centrifugation; thermally treating supernatant; performing high-speed centrifugation again; separating the purified aspartic acid protease inhibitor from the supernatant by a DEAE (Diethylaminoethyl)-52 ion exchange column chromatography; and then freeze-drying under vacuum to obtain the aspartic acid protease inhibitor dry powder. The aspartic acid protease inhibitor prepared by the invention has higher inhibition activity on pepsin and rennin in aspartic acid protease family, is directly extracted from pea, is low in cost and safe in application, and has broad application prospect in the fields of food additives, medicines, agriculture and the like.

The invention relates to a new pepsin-like aspartic acid protease (Napsin A) monoclonal antibody, a preparation method of the hybridoma cell line 21A5, and the application of the monoclonal antibody in tumor occurrence and prognosis evaluation. The antigen for preparing the antibody is a preferred antigenic peptide, which is chemically synthesized and coupled with KLH carrier protein as an immunogen to immunize mice. The finally obtained antibody belongs to IgG2b subtype, and the sequence encoding its variable region is cloned by gene way to get. The immunohistochemical detection of the finally obtained antibody on a variety of tumor tissues found that the antibody can well identify the occurrence of tumors and can be used for the immunological diagnosis of these tumors.

The present invention relates generally to antifungal hydrogels to locally deliver antifungal drugs. Specifically, the present invention provides aspartic protease-triggered antifungal hydrogels to locally deliver antifungal drugs that specifically respond to aspartic proteases secreted by virulent, pathogenic Candida.

The invention provides a preparation method for an aspartic protease inhibitor in enzymatic hydrolysis products of soybean protein isolate, which belongs to the field of separation and purification of biological products. The invention relates to enzymatic hydrolysis of soybean protein isolate with papain for obtainment of a crude product of the aspartic protease inhibitor. The aspartic protease inhibitor is prepared from the crude product by carrying out heat treatment and high speed centrifugation on an enzymatic hydrolysate and carrying out DEAE-52 ion exchange chromatography and separation on an obtained supernatant, and dry powder of the aspartic protease inhibitor is obtained by carrying out vacuum freeze drying on the obtained aspartic protease inhibitor. The aspartic protease inhibitor prepared in the invention has high inhibitory activity on pepsin and chymosin in the family of aspartic protease; the aspartic protease inhibitor extracted from the natural processing material soybean protein isolate which has undergone enzymatic hydrolysis has good specificity, good stability, safety in application and an application prospect in a wide variety of fields, e.g., medicines andhealth care, food additives, control of insect diseases, etc.