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Tissue culture and rapid propagation method of carnation

A technology of carnation group and carnation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of thin stems, vitrification, weak proliferation, etc., and achieve the effect of overcoming thin stems

Active Publication Date: 2013-12-25
YUNNAN JICHUANG HORTICULTURAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of tissue culture and propagation, Carnation has thin and weak stems, yellowing, weak proliferation and severe vitrification, which affect the yield and quality of seedlings and limit the application of tissue culture technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Cut the vitrified carnation seedlings into sections and put them into the following medium: MS+BA 0.05mg / L+KT 0.05mg / L+NAA 0.1mg / L, at a culture temperature of 25°C and a light intensity of 3000lx , under the condition that the light time is 14 hours / day, culture for 25 days, then cut the proliferated tissue culture seedlings into sections, and put them into the following medium: MS+BA 0.05mg / L+NAA 0.1mg / L, at a culture temperature of Under the conditions of 25°C, light intensity of 3000lx, and light time of 14 hours / day, continue to cultivate for 25 days, and then cut the proliferated tissue culture seedlings into the following medium: MS+KT 0.05mg / L+NAA 0.1 mg / L, the culture temperature is 25°C, the light intensity is 3000lx, and the light time is 14 hours / day. Continue to cultivate for 25 days, then cut the proliferated tissue culture seedlings into sections, put them into MS medium, and cultivate The temperature was 25°C, the light intensity was 3000lx, and the ligh...

Embodiment 2

[0028] Cut the non-proliferating carnation plantlets into sections and put them into the following medium: MS+BA 0.15mg / L+KT 0.15mg / L+NAA 0.1mg / L, at a culture temperature of 24°C and a light intensity of 3000lx, under the conditions of 12 hours / day light, culture for 20 days, then cut the proliferated tissue culture seedlings into the following medium: MS+BA 0.25mg / L+NAA 0.1mg / L, at the culture temperature Under the conditions of 24°C, light intensity of 3000lx, and light time of 12 hours / day, the culture was continued for 20 days; after two rounds of culture, the tissue culture seedlings had high reproductive coefficient and good growth, and the tissue culture seedlings with good growth and development were obtained.

Embodiment 3

[0030] Cut the carnation seedlings with weak proliferation into sections and put them into the following medium: MS+BA 0.2mg / L+NAA 0.1mg / L, the culture temperature is 26°C, the light intensity is 2500lx, and the light time is 14 Under the condition of 1 hour / day, continue to cultivate for 30 days, then cut the proliferated tissue culture seedlings into sections and put them into the following medium: MS+BA 0.15mg / L+KT 0.1mg / L+NAA 0.1mg / L. The temperature is 25°C, the light intensity is 2500lx, and the light time is 12 hours / day, and the culture is continued for 25 days, and then the proliferated tissue culture seedlings are cut into sections and put into MS+BA 0.1mg / L+KT 0.15mg / L +NAA 0.1mg / L culture medium, under the conditions of culture temperature 24℃, light intensity 2000lx, and light time 14 hours / day, continue to culture for 30 days; The growth is good, and the tissue culture seedlings with good growth and development are obtained.

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Abstract

The invention provides a tissue culture and rapid propagation method of a carnation. The method comprises the following steps: cutting the hypogenetic tissue culture carnation seedlings into fragments; putting the fragments into different culture mediums to cultivate for 15-30 days alternately, wherein each time, the culture temperature is 22-26 DEG C, the light intensity is 2000-3000lx and the light duration is 10-14 hours per day; cutting the proliferous tissue culture seedlings into fragment and putting the fragments into the next culture medium for further cultivation to obtain well-developed tissue culture seedlings. The tissue culture and propagation method provided by the invention can effectively solve the problems that during the tissue culture and propagation of the carnation, due to the reason of variety or increase of subculture times, the stems of the tissue culture seedlings are thin and delicate, the propagation coefficient is decreased, growth is slow, yellowing, vitrifying, weak propagation ability or no propagation is generated, and the like. The tissue culture and rapid propagation method of the carnation has important significance on carnation germplasm resource preservation and industrialized production of good seedlings.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of carnation, in particular to a method for overcoming weak proliferation and vitrification of carnation tissue culture seedlings, and belongs to the field of biotechnology. Background technique [0002] Carnation ( Dianthus caryophyllus Liunn ), also known as carnation, is a perennial herbaceous plant of the genus Caryophyllaceae in the caryophyllaceae family. It is one of the most widely used flowers in the world and one of the most important export flowers in China and Yunnan Province. Carnation is loved by people for its beautiful flower posture, bright colors and long fresh-keeping period. At present, carnation is in great demand in the market, but conventional cutting propagation and vegetative propagation cannot meet the demand of the market. Tissue culture is an effective way to quickly propagate seedlings in a large number and meet market demand in a short period of t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周旭红桂敏莫锡君苏艳田敏卢珍红吴学尉龙江
Owner YUNNAN JICHUANG HORTICULTURAL TECH
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