A kind of hybridization seed production method of dianthus
A technology of hybrid seed production and carnation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to meet market demand, difficulty in breeding seeds, susceptibility to viruses, etc., to achieve less virus infection and better seed quality , the effect of good plant growth
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specific Embodiment approach
[0032] detailed description Embodiment 1, take Chinese Dianthus male parent and American Dianthus female parent hybridization as example:
[0033] 1. Seedling cultivation
[0034] (1) Female seedling cultivation: choose the stem tip of American Dianthus as the isolated maternal tissue, and conduct tissue culture after the stem tip is sterilized. The tissue culture medium includes differentiation medium, rooting medium and subculture medium. Three Different culture media are prepared separately and used together. The formula of differentiation medium: MS plus BA1.0mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L; the formula of rooting medium: MS plus NAA 0.2 mg / L, sucrose 4000 mg / L, agar powder 550 mg / L; subculture medium formula: big seedling MS plus IBA0.4 mg / L, BA0.5 mg / L, sucrose 4000 mg / L, agar Agar powder 5500 mg / L, seedling MS plus BA0.5 mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L and MS plus BA1.0 mg / L, NAA0.4 mg / L L, sucrose 4000 mg / ...
Embodiment 2
[0054] Embodiment 2, take American Dianthus male parent and Chinese Dianthus female parent hybridization as example:
[0055] 1. Seedling cultivation
[0056] (1) Female seedling cultivation: choose the stem tip of Dianthus chinensis as the isolated maternal tissue, and conduct tissue culture after the stem tip is sterilized. The tissue culture medium includes differentiation medium, rooting medium and subculture medium. Three Different culture media are prepared separately and used together. The formula of differentiation medium: MS plus BA1.0mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L; the formula of rooting medium: MS plus NAA 0.2 mg / L, sucrose 4000 mg / L, agar powder 550 mg / L; subculture medium formula: big seedling MS plus IBA0.4 mg / L, BA0.5 mg / L, sucrose 4000 mg / L, agar Agar powder 5500 mg / L, seedling MS plus BA0.5 mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L and MS plus BA1.0 mg / L, NAA0.4 mg / L L, sucrose 4000 mg / L, agar powder 5500 mg...
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