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Acer negundo aurea tissue culture rapid propagation method

The invention relates to a technique for rapid tissue culture and tissue culture of A. chinensis, which is applied in the field of plant tissue culture and can solve the problems of complex tissue culture process and the like, and achieve the effects of simple tissue culture process, robust growth and simple culture process.

Active Publication Date: 2015-04-08
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention utilizes tissue culture technology to obtain Acer aureus aureus, with a reproduction coefficient of 3 to 6. The tissue culture process is simple, time-saving and labor-saving, solves the problem of complex tissue culture processes in the prior art, and provides a The method of tissue culture and rapid propagation of Acer oleifera provides technical support for the industrial seedling cultivation of Acer oleifera

Method used

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  • Acer negundo aurea tissue culture rapid propagation method
  • Acer negundo aurea tissue culture rapid propagation method
  • Acer negundo aurea tissue culture rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: A method for tissue culture and rapid propagation of Acer quinquefolia includes the following steps:

[0028] (1) Sterilization of explants: Collect the young stems of Acer platyphylla without diseases and insect pests, remove the leaves, cut the stems into 2cm shoots with buds, and sterilize them with alcohol for 30s on a clean bench. Sterilize with mercury for 5 minutes, rinse with sterile water 4 times;

[0029] (2) Start-up culture: inoculate the sterilized stem with buds into the start-up medium, cultivate in the culture room for 2 weeks, and the axillary buds will extend 2cm;

[0030] (3) Primary culture: Cut off the axillary buds, inoculate them into the primary culture medium, and cultivate them in the culture room for 3 weeks to obtain robust rooted Acer platyphylla aseptic tissue culture seedlings. After 3 weeks of culture, the plants grow 6 cm. And rooting, the root system is developed, the number of roots is 2, the root length is 3cm, and the axillary ...

Embodiment 2

[0036] Example 2: A method for tissue culture and rapid propagation of Acer quinquefolia includes the following steps:

[0037] (1) Sterilization of explants: Collect the young stems of Acer platyphylla free of diseases and insect pests, remove the leaves, cut the stems into 3cm stems with buds, and sterilize them with alcohol for 60s on a clean bench. Sterilize with mercury for 8 minutes, rinse with sterile water 6 times;

[0038] (2) Start-up culture: Inoculate the sterilized stem with buds into the start-up medium, cultivate in the culture room for 3 weeks, and the axillary buds will extend 4cm;

[0039] (3) Primary culture: Cut off the axillary buds, inoculate them into the primary culture medium, and cultivate them in a culture room for 2 weeks to obtain robust rooted Acer spp. aseptic tissue culture seedlings. After 4 weeks of culture, the plants grow 7 cm. And rooting, the root system is well-developed, the number of roots is 12, the root length is 5cm, and the axillary buds...

Embodiment 3

[0045] Example 3: A method for tissue culture and rapid propagation of Acer quinquefolia includes the following steps:

[0046] (1) Sterilization of explants: collect the young stems of Acer platyphylla without pests and diseases, remove the leaves, cut the stems into 2.3cm stems with buds, and sterilize them with alcohol for 40s on a clean bench , Sterilized with mercury for 6min, rinsed with sterile water 5 times;

[0047] (2) Start-up culture: inoculate the sterilized stem with buds into the start-up medium, cultivate in the culture room for 16 days, and the axillary buds will extend 2.8cm;

[0048] (3) Primary culture: Cut off the axillary buds, inoculate them into the primary culture medium, and cultivate them in the culture room for 16 days to obtain robust rooted Acer palmatus aseptic tissue culture seedlings. After 23 days of culture, the plants grow 6.3 cm. And rooting, the root system is well-developed, the number of roots is 6, the root length is 3.5cm, and the axillary ...

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Abstract

The invention discloses an acer negundo aurea tissue culture rapid propagation method which belongs to the technical field of plant tissue culture, and includes explant sterilization, starting culture, primary culture, subculture and transplanting steps. Only one medium is used in the method, and the medium is 1 / 2DKW+0.1mg / L NAA (naphthalene acetic acid) +0.05mg / L IAA (indole acetic acid) + 30g / L sucrose + 5g / L agar, proliferation and rooting culture can be performed on the same medium, and the method has the characteristics of being simple in culture process, easy to operate, high in propagation coefficient, short in breeding cycle, free of hardening, and high in survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture method, in particular to a tissue culture rapid propagation method of Acer aurantiaceae. Background technique [0002] Acer negundo 'Aurea' is a plant belonging to the genus Acer in the Aceraceae family. The golden leaf maple is a tall deciduous tree, a cultivar of the maple leaf, which was introduced from Europe. The pinnate compound leaves of the golden leaf maple are very large, the leaf color is soft, and it is golden yellow in spring; the leaves are not scorched in summer; the cold resistance is strong, and it can withstand low temperatures from -40 degrees to -45 degrees; it has a wide range of suitable growth, It can be planted in Shenyang, Jiangsu, Zhejiang, and South China in Northeast China. It likes soil with good air permeability, and can be planted alone or in groups; it has strong growth potential and germination power, with an annual gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 沈香兰马建华王小辉朱晓菲马良良杨小霞
Owner SICHUAN COLORLINK CO LTD
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