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Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide

A technology of plant growth-promoting bacteria and microorganisms, which is applied in the field of plant growth-promoting bacteria and their agents, and their preparation, and in the field of plant growth-promoting agents. To avoid problems such as the difficulty of obtaining species, to achieve the effect of comprehensive growth-promoting performance, single strain, and low cost

Inactive Publication Date: 2011-04-27
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since it is difficult to obtain strains with a variety of high-efficiency growth-promoting effects at the same time, most of the plant growth-promoting agents for dissolving phosphorus and nitrogen-fixing plants that are currently put into production are multi-strain mixed agents, and there is antagonism between strains, and the nutritional requirements in the agent and Metabolic imbalance etc.

Method used

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  • Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide
  • Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide
  • Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide

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Embodiment 1

[0020] Klebsiella pneumoniae ( Klebsiella pneumoniae ) isolation, identification and purification: The RSN19 strain was sterilized from surface-sterilized red bean grass ( Onbrychis viciaefoliacv. ) isolated from root nodules, and the purified strain was morphologically identified as conforming to the general definition of Enterobacteriaceae. The pure culture of the bacterial strain was identified as Klebsiella pneumoniae (Klebsiella pneumoniae) (No. 084 in 2010 Weijianzi) by the Microorganism Identification and Preservation Center of the Chinese Academy of Sciences in 2010; Ampicillin was tolerated. The nitrogenase activity of the RSN19 strain reached 6695 ± 174 nmol C2H2 d-1, the dissolved phosphorus (calcium phosphate) was 15.29 ± 0.46 mg / L at 4 days, and the auxin production was 8.38 mg / L at 6 days. In the sand culture test under the condition of no nitrogen and only providing calcium phosphate, the biomass of alfalfa seedlings after applying RSN19 bacterial solution fo...

Embodiment 2

[0022] A preparation method of a plant growth-promoting agent is carried out in accordance with the following steps:

[0023] (1) Klebsiella pneumoniae ( Klebsiella pneumoniae ) RSN19 was inoculated in YMA (yeast mannitol-agar) solid medium and activated at 26°C-28°C for 18-24h;

[0024] (2) Activated Klebsiella pneumoniae RSN19 was inoculated in a liquid medium for fermentation at a temperature of 25°C to 29°C, and cultured to a concentration of Klebsiella pneumoniae The number of RSN19 bacteria is 4~6??10 10 indivual;

[0025] (3) In the above step (2) Klebsiella pneumoniae RSN19 fermentation broth, sterile liquid medium, and 10% pH 7.0 sterile phosphate buffer are mixed in a volume ratio of 6:3:1; packaged in sterile aluminum foil bags or sterile polypropylene bags, Make a single-species liquid bacterial agent;

[0026] (4) Mix the liquid inoculum prepared in the above step (3) with sterile peat powder at a volume ratio of 1:6 to make a solid microbial inoculum.

...

Embodiment 3

[0029] The growth-promoting effect of the bacterial agent of the present invention in sand culture under the condition of controlling nitrogen and phosphorus nutrition

[0030] 1. Experimental materials

[0031] 1.1 Test material

[0032] Liquid bacterial agent of the present invention: Klebsiella pneumoniae ( Klebsiella pneumoniae ), its microbial deposit number is: CGMCC No. 4128.

[0033] 1.2 Plant material

[0034] The seeds of alfalfa Longdong (Medicogosativa. cv. LongDong) and Queen (Medicogo. sativa. cv. Queen), Gansu red bean grass (Qnobrychisspp. cv. Gansu) and oat (Calibre) (Avena. sativa L. Calibre) were obtained from Provided by the Key Laboratory of Grassland Ecosystem Ministry of Education, the seed purity is 98%, and the seeds are sterilized with 70% ethanol solution for 5 minutes. In order to eliminate the influence of rhizobia and nitrogen-fixing bacteria on the experimental results, the seeds were soaked in sterile water to accelerate germination, the see...

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Abstract

The invention discloses growth-promoting rhizobacteria and fungicide thereof, as well as a preparation method and application of the fungicide, namely Klebsiellapneumoniae and the fungicide taking the Klebsiellapneumoniae used as an active ingredient, the preparation method of the fungicide and application of the fungicide in phosphorus dissolving and nitrogen fixation. The invention has the advantages that the nitrogen fixing and phosphorus dissolving bacterium integrated with multiple growth-promoting capabilities, such as phosphorous dissolving, nitrogen fixing and auxin producing, into a whole is provided to prepare the nitrogen fixing and phosphorous dissolving fungicide, the fungicide has the characteristics of complete growth promotion performance, high fertilizer efficiency and low cost, and is suitable for the regions lacking nitrogen and phosphorous and having hungry soil; and the preparation process is simple and convenient, high fertilizer efficacy of nitrogen fixing and phosphorous dissolving is obtained, and the invention has excellent development prospect.

Description

technical field [0001] The invention relates to a plant growth-promoting bacterium agent, in particular to a plant growth-promoting bacterium and its bacterium agent, as well as its preparation method and application, belonging to the field of biological fertilizers. Background technique [0002] As essential mineral elements for plants, nitrogen and phosphorus are important components of various structural and functional substances such as proteins, nucleic acids, phospholipids, enzymes, and plant hormones in plant living cells. It plays an important role in the process of gene expression and regulation and cell signal transduction. The lack of nitrogen and phosphorus will inhibit the growth and development of plants, resulting in a decline in crop yield and quality. Long-term large-scale application of chemical fertilizers not only increases the cost of agricultural production, but also causes the selective accumulation of certain elements in the soil and the deterioratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/22
Inventor 师尚礼李剑峰张淑卿霍平慧
Owner GANSU AGRI UNIV
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