Method for improving cadmium resistance of tomatoes
A technology of tomato and cadmium resistance, applied in the fields of land preparation methods, horticultural methods, botanical equipment and methods, etc., can solve the problem of less research on synergistic promotion, and achieve the effect of reducing potential risks, improving cadmium resistance and strong cadmium resistance.
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[0032] The preparation process of the tested bacterial agent: Soil samples were collected from the rhizosphere of many common crops polluted by acid mine wastewater in Shangba Village, Guangdong Province. The soil suspension was inoculated into LB solid medium (10.0 g of bacteriological peptone, 5.0 g of yeast extract, 10.0 g of NaCl, 1 L of deionized water, and 1 L of agar) added with 30 mg / L Cd(II) by dilution coating 15g, pH 7.0), pick a single colony of bacteria with different shapes and plump growth on the plate, and streak on the solid medium containing Cd(II) (30mg / L), enrich and purify continuously for 2-3 times, and then Obtain pure cultures of Cd-tolerant bacteria. After separation and purification, the test strain was spread on the LB solid medium plate, and incubated at 30°C for 48 hours, and a single colony with good separation was selected and sent to the biological company for 16SrRNA strain identification. Two strains of bacteria PGPR (Enterobacter sp. (herein...
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