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Application of Pseudoalteromonas in Preparation of Nanomaterials

A technology of pseudoalteromonas and nanomaterials is applied in the application field of pseudoalteromonas in the preparation of nanomaterials, which can solve the problems of chemical sludge consumption of chemical agents, high operating costs, complicated processes, etc. Cadmium performance, saving raw materials, simple operation effect

Active Publication Date: 2019-02-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biological treatment of cadmium and phosphorus in wastewater mainly relies on adsorption and anaerobic phosphorus removal, the process is complicated, and it is necessary to control aeration conditions and sludge age.
In terms of high-salt seawater wastewater treatment, the method of acclimating salt-tolerant activated sludge is mainly used, but it takes a long time and high operating costs. The high-efficiency sedimentation biological filtration process based on chemical precipitation consumes a large amount of chemical agents and produces chemical sludge.

Method used

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  • Application of Pseudoalteromonas in Preparation of Nanomaterials
  • Application of Pseudoalteromonas in Preparation of Nanomaterials
  • Application of Pseudoalteromonas in Preparation of Nanomaterials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS prepares nanomaterials in low-salt wastewater

[0037] (1) Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS was inoculated in liquid LB medium at 0.9% (v / v), and cultured in a constant temperature shaker at 25° C. and 200 rpm for 20 hours to obtain enriched bacteria.

[0038] (2) The enriched bacterial suspension was inoculated in low-salt wastewater at 10% (v / v), and cultured in a constant temperature shaker at 25°C and 200rpm for 48 hours to obtain a bacterial suspension containing cadmium-phosphorus-sulfur nanoparticles. liquid.

[0039](3) The bacterial suspension containing cadmium-phosphorus-sulfur nanoparticles was centrifuged and washed with deionized water. The centrifugation speed is 3000rpm, and low-speed centrifugation is used to prevent the elution of particles on the surface of the bacteria, and the nanomaterials are prepared.

[0040] Described liquid LB culture medium component is as fo...

Embodiment 2

[0047] Embodiment 2: Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS prepares nanomaterials in high-salt wastewater

[0048] (1) Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS was inoculated in liquid LB medium at 0.9% (v / v), and cultured in a constant temperature shaker at 25° C. and 200 rpm for 20 hours to obtain enriched bacteria.

[0049] (2) Centrifuge the enriched bacteria at 3000 rpm for 10 min, discard the supernatant, and resuspend to the original volume with high-salt wastewater to obtain the bacteria washed once.

[0050] (3) Inoculate the washed bacterial suspension into three groups of high-salt wastewater at 0.2‰ (v / v), and cultivate it in a constant temperature shaker at 25°C and 200rpm for 48h to obtain cadmium-phosphorus-sulfur nanoparticles. bacterial suspension.

[0051] (4) The bacterial suspension containing cadmium-phosphorus-sulfur nanoparticles was washed with deionized water at a centrifugation speed of 3000 rpm, and low-speed centrifugation wa...

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Abstract

The invention discloses the application of Pseudoalteromonas sp. DSBS in the preparation of nanomaterials in nM-level unsaturated high-salt wastewater containing cadmium and phosphorus; the Pseudoalteromonas DSBS has been used on December 12, 2013 Preserved in the China Type Culture Collection Center, address: Luojia Mountain, Wuchang, Wuhan City, preservation number: CCTCC M2013652. The invention also discloses a cadmium-phosphorus-sulfur nanomaterial, which is biosynthesized by Pseudoalteromonas DSBS in nM-level unsaturated high-salt wastewater containing cadmium and phosphorus. The bacterial strain of the invention can synthesize nanoparticles in different salinities and cadmium-phosphorus unsaturated systems, and achieve the removal of cadmium and phosphorus in water bodies. The substrate utilization rate is high and the effluent is free of cadmium and phosphorus pollution.

Description

technical field [0001] The invention relates to the application of pseudoalteromonas in the preparation of nanometer materials. Background technique [0002] Nanomaterials have unique physical characteristics and chemical properties, and can be used in the fields of sensors, catalysis, semiconductors, drug delivery, pollutant degradation, and environmental remediation, and have attracted extensive attention from academic and engineering circles in recent years. [0003] At present, the synthesis of metal nanomaterials mainly includes physical, chemical and biological methods. Physical methods include evaporation and condensation, physical pulverization, and mechanical alloying, but their output rate is low, energy consumption is high, and cost is high. Chemical methods include chemical precipitation, hydrothermal synthesis, template method, etc. However, it is not easy to control the size, shape and structure of the synthesized nanoparticles; it needs harsh conditions such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12N1/20C02F3/34B82Y40/00B82Y5/00B01J27/14C12R1/01C02F101/20C02F101/14C02F101/10
CPCC12N1/20C12P3/00B82Y5/00B82Y40/00C02F3/34B01J27/14C02F2101/14C02F2101/20C02F2101/101C02F2101/006C02F2101/105B01J35/39Y02P20/59Y02P20/133
Inventor 周维芝王美张海欧刘东生赵海霞
Owner SHANDONG UNIV
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