66 results about "Pseudoalteromonas sp." patented technology

The invention relates to a chitinase gene chiC and encoded protein and application thereof, and belongs to the technical field of genetic engineering. According to the chitinase gene chiC and the encoded protein and the application thereof, pseudoalteromonas sp. DL-6 serves as a research object, the chitinase gene chiC with a nucleotide sequence showed in SEQ ID No 1 is cloned for the first time, and a high-activity chitinase is obtained. The preparation method of the high-activity chitinase is simple, the production cost is low, a product is easy to conserve, the appropriate hydrolysis temperature is 30 DEG C, The enzyme-decomposed PH is 9.0, high degradation activity of a colloidal chitin can reach to 1.569 + / - 0.017 U / ml, the chitinase chiC degrades an aipha chitin and a beta chitin to generate a chitobiose, and industrial production prospect is possessed.

The invention discloses a method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam, and relates to a microorganism adsorption and immobilization method. The invention provides a method for immobilizing the pseudoalteromonas sp. SP48 by the polyurethane foam, which can effectively overcome the defect of immobilizing cells by an entrapment method, and makes the pseudoalteromonas sp. SP48 show high-efficiency alga killing activity effectively and durably by taking the polyurethane foam as an adsorption carrier. Pseudoalteromonas sp. SP48-containing polyurethane foam is formed by sterilizing granulated, boiled and dried polyurethane foam and an alga killing bacterium culture medium together, and inoculating the SP48 for shaking culture to ensure that the SP48 enters or attaches to the inner wall of the polyurethane foam for growth. The SP48 is adsorbed and immobilized by the polyurethane foam which has a certain density and size, high adsorption capacity, no toxic or side pollution, good mass transfer and energy transfer effects, convenience and low cost; application performance in actual red tide treatment is improved remarkably; and the used polyurethane foam can be recycled through treatment, which achieves good economic benefit.

The invention relates to pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and an application thereof. Pseudoalteromonas sp. DSBS is collected in the China Center for Type Culture Collection (CCTCC) in Luojiashan, Wuchang, Wuhan City on December 12, 2013, with collection number of CCTCC M2013652. The strain has cadmium resistance and can still grow in cadmium-containing wastewater, and the live thallus can take full advantage of the characteristics of heavy metal adsorption and absorption, simultaneously absorbs phosphorus or forms Cd-P precipitates around the thallus in the growth process and synchronously removes cadmium and phosphorus. The toxicity of cadmium towards the strain is reduced in the presence of phosphorus.

The invention discloses a marine Pseudoalteromonas sp. B8453013 (strain collection number CGMCC No.3281) of low-temperature proteinase, a production method for producing low-temperature proteinase from the strain and a low-temperature proteinase. A system method for strain collection, rejuvenation and seed selection is built by alternating Pseudoalteromonas sp. B8453013; the method for producing the low-temperature proteinase is built by culture medium optimization screening and the optimized control of fermentation technology conditions; and the produced low-temperature proteinase has the advantages of good low-temperature activity and high enzyme activity. The low-temperature proteinase can be widely applied to industries, such as detergents, feed, fur, food processing and the like.

The invention discloses pseudoalteromonas sp.QN-1 with a low-temperature petroleum degradation function, and the collection number is CGMCC No. 11635. The pseudoalteromonas is selected from petroleum-polluted seas in north China, can degrade petroleum at a low temperature, and particularly, can degrade naphthalene in the petroleum at the extreme temperature of 0 DEG C. The pseudoalteromonas QN-1 can be applied to biological remediation of ocean petroleum pollution, and particularly, can be applied to naphthalene degradation in low-temperature environments, such as in winter (including glacial periods), at seas in north China.

The invention relates to pseudoalteromonas (highly active gellan gum oligosaccharide producing bacteria) and a use thereof. Specifically, the invention provides pseudoalteromonas PE2, namely pseudoalteromonas sp., wherein the preservation number is CGMCC NO:16439. The invention also provides the use of the pseudoalteromonas PE2 for producing gellan gum lyase and producing gellan gum oligosaccharides.

The invention discloses a drinking water heavy metal efficient adsorbent. The adsorbent contains exopolysaccharides which is generated by synergistic fermentation of pseudoalteromonas GHS18 with the preservation number being CGMCC No.12913 and pseudoalteromonas JP with the preservation number being CGMCC No.12914. The drinking water heavy metal efficient adsorbent has the following beneficial effects: growth and propagation between the pseudoalteromonas sp. GHS18 and the pseudoalteromonas sp. JP have remarkable synergistic effect, the adsorption effect of the adsorbent prepared from the obtained exopolysaccharides on the heavy metal in water is better than that of the adsorbent prepared from the exopolysaccharides obtained through fermentation of single marine bacteria, and remarkable progress is achieved. The prepared adsorbent can adsorb various kinds of heavy metal such as cadmium, lead and copper in the drinking water, has high adsorption efficiency and stable performance, is green and safe, and has high economic value.

The invention aims to provide a scophthalmas maximus antagonistic bacterium, which is pseudoalteromonas sp. is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology of Chinese Academy of Sciences in No. 1 Beichen West Road, Chaoyang District, Beijing on July 3rd, 2013 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7870. A bacterium which is screened by the invention with fin bleeding and deadly diseases of scophthalmas maximus is strong in antagonistic activity, and the antagonistic bacterium extracellular products prepared by utilizing a salting out method are stable in yield, low in cost and high in antagonistic activity. By using the antagonistic bacterium or antagonistic products, the immune protective rate for scophthalmas maximus is 50-60 percent; the antagonistic product is a pure biological preparation, has the characteristics of being safe and environmentally-friendly, is prevented from medicinal residues and environmental pollution; the method can be used for inhibiting occurrence and popularization of fin bleeding deadly epidemic diseases of scophthalmas maximus.

The invention relates to a preparation method of a microbial bactericide, which belongs to the field of microbial fermentation industry. The invention discloses a method for preparing Vibrio anguillaris bactericide by using Pseudoalteromonas sp., which consists of (1) slant activation and culture of strains, (2) liquid fermentation culture, (3) crude fermented liquid Mention, (4) steps such as the preparation of bacteriostatic agent are formed. The method of the invention is simple, the culture medium is cheap and easy to prepare, and the liquid culture technology is adopted, which is beneficial to enlargement and industrialized production, and has stable properties and good application value.

The invention discloses an application of pseudoalteromonas sp. DSBS to preparation of a nano-material. The pseudoalteromonas sp. DSBS is collected in China Center for Type Culture Collection (CCTCC) on December 12, 2013, with an address of Luojiashan, Wuchang, Wuhan and a collection number of CCTCC M2013652. The invention furthermore discloses the cadmium-phosphorus-sulfur nano-material, which is biologically synthesized from the pseudoalteromonas sp. DSBS in a water body containing cadmium and phosphorus. According to a strain, nanoparticles can be synthesized in cadmium-phosphorus unsaturated systems with different salinities, and cadmium and phosphorus in the water body can be removed. The substrate utilization rate is high and outlet water is free of cadmium and phosphorus pollution.

The invention discloses pseudoalteromonas polysaccharide degrading bacteria as well as a culture method and application thereof. The pseudoalteromonas sp. Q08 is preserved in China General Microbiological Culture Collection Center in Institute of Microbiology, Chinese Academy of Sciences No. 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, with the preservation number being CGMCC No.16720. The strain can grow by taking microbe, alga, land plants and various types of animal-derived polysaccharide as an only carbon source, ensures that the species of produced polysaccharide degrading enzyme is rich, belongs to multifunctional polysaccharide degrading bacteria, and ensures that extracellular enzyme preparations prepared from the strain degrade microbe, alga, land plants and animal saccharides, and is obvious in degradation activity for sodium alginate and agarose from alga and HA, CSC and CSE from animals.

The invention aims at providing a marine bacterium Pseudoalteromonas sp SC127 and an ulva sulfuric acid bhamnosan enzyme prepared from the same. The preservation number of the strain is CCTCC NO. M 2015422, and the strain has the capability of producing the sulfuric acid bhamnosan enzyme and can be used for preparing oligomeric sulfuric acid bhamnosan. It is determined through enzyme separation and purification and SDS-PAGE that the molecular weight of the sulfuric acid bhamnosan enzyme produced from the strain is 110.6 kDa. The strain serving as a production strain of the sulfuric acid bhamnosan enzyme has the advantages of being low in nutritional requirement, high in enzyme production activity, good in stability, short in production cycle and low in cost, has certain application value in industrial production and provides a guarantee for activity research and development of oligosaccharide.

The invention relates to a chitin binding protein CBP58, and an encoding gene and an application thereof, and belongs to the technical field of gene engineering. The encoding gene of the chitin binding protein CBP58 is cloned from a strain pseudoalteromonas sp.DL-6 for the first time; and a high-activity expression product is obtained by construction of a prokaryotic expression vector and imidazole elution. The preparation process is simple; a simple and convenient method is provided for subsequent research on the chitin protein CBP58; the chitin protein CBP58 is low in production cost and easy to store, has a chitin binding function, is applied to purification and auxiliary identification of chitin, has a good inhibiting effect on aspergillus niger (Aspergillus niger) and cytospora mandshurica miura (Cytospora mandshurica miura), has a potential biological control function and has wide application value; a novel research direction is provided for research and development of anti-fungal medicines in the field of agriculture; and great industrial benefits and economic value are generated.

The invention discloses salt-tolerant Pseudoalteromonas sp and application thereof, and belongs to the technical field of coastal zone microbial technology and marine aquaculture wastewater treatment.The Pseudoalteromonas sp is Pseudoalteromonas hodoensis S2-M-5, which is isolated from sediment near a sewage outlet of Jinhai Bay Beach in Weihai City, Shandong Province, and is preserved in the General Microbiology Center of the China Microbial Culture Collection Management Committee, and the preservation number is CGMCC No. 14429. After inoculating the Pseudoalteromonas hodoensis S2-M-5 is inoculated in a suitable fermentation medium for shaking fermentation culture, the flocculation rate of a bacterial treatment suspension reaches 78.20%, the COD removal rate is 50% or more, the ammonia nitrogen removal rate is 21.27%, and the nitrite nitrogen removal rate is 40.19%. The Pseudoalteromonas hodoensis S2-M-5can be applied to the treatment of marine aquaculture wastewater due to salt tolerance and safety.

The invention discloses a method for preparing a flocculating agent by use of a compound microbial agent. A remarkable synergetic function is produced on growth and reproduction of Pseudoalteromonas sp. GHS18 and Pseudoalteromonas sp. JP, and the prepared flocculating agent has the decolorization flocculation effect superior to that obtained through fermentation by single strains. The preparationmethod of the flocculating agent comprises steps as follows: culture of a seed solution, preparation of a liquid culture medium, expanded fermenting culture and preparation of the flocculating agent.A hydrolysate obtained by hydrolyzing crop waste with inorganic acid is used for replacing a carbon source in the culture medium, and the cost is saved; sandy soil is taken as a carrier, so that the strains are attached to the carrier, and effective ingredients of the flocculating agent can be simply and thoroughly separated from a culture solution.

The invention discloses a cypermethrin- and / or deltamethrin- degrading bacterium in seawater environment, and separation, purification and applications thereof. The bacterium, and the separation, the purification and the applications thereof are characterized in that: the bacterium is an HS-10 bacterial strain and is classified and named as Pseudoalteromonas sp.; the bacterium is preserved in the common microbe center of China Committee for Culture Collection of Microorganisms on 24 April 2012; the accession number of the bacterium is CGMCCNo.6044; the Genbank accession number of the 16SrDNA of the bacterium is JX087926; and the bacterium is advantageous in that the bacterium can safely, efficiently and rapidly degrade cypermethrin and deltamethrin in the seawater environment, and the bacterium has a good application prospect in the fields of offshore environment protection, treatment of pesticide residue pollution in a breeding environment and food safety guaranteeing.

The invention discloses a bacterial exopolysaccharide-based drinking water heavy metal adsorbent, which is prepared from exopolysaccharides produced by a marine bacterium Pseudoalteromonas sp. GHS18 strain with the preservation number of CGMCC No. 12913, agar and starch, wherein the mass ratio of the agar to the starch is (1 to 2) : (2 to 1). The beneficial effects are as follows: the prepared adsorbent can adsorb various heavy metal such as cadmium, lead and copper in drinking water, has the advantages of high adsorption efficiency and stable performance, and overcomes the defects of potential toxicity of inorganic polymeric adsorbents and synthetic organic polymeric adsorbents to organisms, thus ultimately achieving non-polluting emission. When being used, the adsorbent just needs to be put into the drinking water, the use is convenient, and drinking water quality is improved.

The invention provides a method for preparing dextran enzyme by utilizing marine bacteria Pseudoalteromonas sp.LP621. The method is characterized in that: the method comprises the following steps that: strains of the Pseudoalteromonas sp.LP621 are inoculated to a 2216E culture medium under the conditions of rotating speed of 180r / min, liquid volume of 20 percent and culture time of 16h so as to obtain a seed liquid; according to 2 percent of inoculum size, the seed liquid is inoculated to a fermentation medium, and under the condition of rotating speed of 180r / min, the seed liquid is cultured at a temperature of 25 DEG C for 24h, and under the condition of rotating speed of 10,000r / min, the seed liquid is centrifuged for 5min, and the precipitations are thalli; and the thalli are suspended in a buffer solution, placed in a ice water bath for ultrasonic crushing for 5min, and centrifuged for 10min under the condition of rotating speed of 12,000r / min, and a supernatant is a coarse enzyme liquid of the dextran enzyme. The invention also relates to a dextran enzyme product obtained by the method, and the dextran enzyme product plays important role in the fields of prevention and treatment of a decayed tooth, sugar industry, production of pharmaceutical dextran and the like.

The invention relates to a strain of Pseudoalteromonas sp. and application thereof, in particular to a strain of Pseudoalteromonas sp. SM20429 and the application thereof, which belong to the technical field of marine organisms. The Pseudoalteromonas sp. SM20429 was collected in China Center for Type Culture Collection (CCTCC) on 19 September, 2010, wherein the address is Wuhan University in China and the strain collection number is CCTCC M 2010239. The invention also relates to an autonomous replication expression vector pWD used for the Pseudoalteromonas sp. SM20429, and a construction method and the application thereof. The nucleotide sequence of the autonomous replication expression vector pWD is shown as SEQ ID NO.1. The strain SM20429 has a typical psychrophilic characteristic and can grow quickly at the low temperature of 15 DEG C. Therefore, the strain SM20429 has a potential value of being developed into a psychrophilic expression host capable of largely expressing foreign proteins to fulfill the aim of preparing psychrophilic protein quickly at the low temperature.

The invention belongs to the technical field of microbiological research and relates to a melanin-producing marine bacterium and a fermentation method for producing natural melanin. The melanin is extracted from the culture product of a marine bacterium. The bacterium needs to be cultured in a medium containing L-tyrosine (L-Tyr) and supplemented with glucose, peptone, beef extract or beef powder and aged seawater, under such a condition that the initial pH value ranges from 6.1 to 7.8. The culture product is subjected to high-speed centrifugation to remove the bacterium, the pH value of the supernatant is adjusted to the range from 2 to 4, the supernatant is incubated at room temperature and then centrifuged to remove foreign matters, and the resulting product is dried until the weight is constant and then eluted with organic solvents to produce the purified melanin (i.e. natural melanin). The bacterium is a strain of Pseudoalteromonas sp. and has a preservation serial number of CGMCC No.1782. The natural melanin has radioprotective and antioxidant effects. The production method has low cost and high yield and can be widely applied.

The invention relates to a Pseudoalteromonas sp. LP602 CGMCC No.4319. The strain is characterized in that the strain is a Gram-negative rod-shaped bacterium, and has capsules but no spores; and the growth temperature range of the strain is 4-37 DEG C, the growth pH range is 6.0-11.0, and the grown NaCl concentration range is 0.5-10%. The invention also discloses a method for producing a dextran enzyme by using the Pseudoalteromonas sp. and a dextran enzyme product prepared by the method. The dextran enzyme plays an important role in the aspects of saprodontia prevention and treatment, medicaldextran production and the like.

The invention discloses a culture medium for producing carrageenase by using fermentation of edible pelvetia silquosa pseudoalteromonas (Pseudoalteromonas sp.) ASY5. The culture medium comprises a strain activation medium, a seed medium and a fermentation medium. The invention also discloses a fermentation method for producing the carrageenase by using fermentation of edible pelvetia silquosa pseudoalteromonas. On the basis of shake-flask fermentation, the screened edible pelvetia silquosa pseudoalteromonas is subjected to fermentation cultivation in a fermentation tank. The enzyme producing rule of the pseudoalteromonas in the fermentation tank is researched, the yield of the carrageenase is effectively improved, and the carrageenase produced by using fermentation of edible pelvetia silquosa pseudoalteromonas (Pseudoalteromonas sp.) ASY5 is subjected to pilot plant test scale-up fermentation, and important technical parameter guidance is provided for large-scale production in the future.

The invention relates to the field of environmental treatment, and in particular relates to sulfur oxidizing bacteria and application thereof. The sulfur oxidizing bacteria are Pseudoalteromonas sp. LZB5-2, and have been preserved in the China General Microbiological Culture Collection Center on January 29, 2021; the preservation address is Beijing, China; the preservation number is CGMCC NO.21762; and the classification name is Pseudoalteromonas sp. According to the invention, sludge, sewage waste and the like which cannot be directly utilized are comprehensively utilized by utilizing microorganisms; and the invention can also be applied to aquatic product culture.

The invention discloses a strain of Pseudoalteromonas marina, and a Pseudoalteromonas marina decolorization flocculant preparation method, wherein the is Pseudoalteromonas marina separated and screened from the mud discharged by Ruditapes philippinarum bred in the marine farm of Zhujiajian, Zhoushan, Zhejiang, China, and the identifying results show that the strain is Pseudoalteromonas sp.. The preparation method comprises: seed liquid culture, liquid culture medium preparation, expansion fermentation culture, and flocculant preparation. According to the present invention, the carbon source inthe culture medium is replaced with the hydrolysis liquid obtained through the crop waste hydrolysis with the inorganic acid, such that the cost is saved; the sandy soil is used as the carrier, and the bacteria are adhered to the carrier, such that the effective components of the flocculant can be simply and completely separated from the culture liquid; and the prepared decolorization flocculanthas advantages of less consumption, high decoloration rate, large floc forming, quick settling, stable performance, good decolorization flocculation effect, no potential toxicity to organisms, healthand environmental protection.

The invention discloses a method for quantitatively detecting the activity of L-amino acid oxidase by using a Prussian blue agar plate, which comprises the following steps: with a punch, punching a small hole having a diameter of 4-10 mm on the Prussian blue agar plate; then, adding a liquid to be determined into the small hole, and standing at room temperature for 10-30 minutes, wherein hydrogen peroxide generated by the liquid to be determined forms a blue ring around the small hole; and determining the diameter of the blue ring, determining the release amount of hydrogen peroxide according to the hydrogen peroxide and a standard straight line made of the diameter of the blue ring, and then deducing to obtain the activity of L-amino acid oxidase, wherein the liquid to be determined is a reaction liquid obtained by reacting an enzyme source and a substrate under the conditions of a temperature of 25-50 DEG C and a pH value of 5-9 for 0.5-2 hours, the enzyme source is an enzyme obtained through the fermentation culture of Pseudoalteromonas sp. B3, and the substrate is L-amino acid. The method has the characteristics of low cost, simple and convenient operation process, wide applicability, high stability and the like.

The invention discloses a culture medium for Pseudoalteromonas sp.DHQ25 and a preparation method thereof, relates to a culture medium, and provides a culture medium suitable for culturing the Pseudoalteromonas sp.DHQ25 and capable of obviously improving cell density of the Pseudoalteromonas sp.DHQ25, and a preparation method thereof. The culture medium consists of 13 to 13.5g / L of peptone, 4 to 4.5g / L of yeast powder, 0.05 to 0.15g / L of glucose, 20 to 30g / L of NaCl, 0.1 to 0.3g / L of MgSO4.7H2O, 0.4 to 0.6g / L of CaCl2.2H2O, 0.03 to 0.09g / L of KCl, 0.04 to 0.06g / L of K2HPO4, 0.04 to 0.06g / L of KH2PO4, and 0.0015 to 0.0025g / L of Fe2(SO4)3, and distilled water is used for fixing the volume of 1L. The preparation method comprises the following steps of: designing an experiment by using power builder (PB); performing a steepest ascent experiment; and performing a central composite design.

The invention discloses Pseudoalteromonas sp.DC-2 having a low-temperature petroleum degrading function and collected under CGMCC No.11633. The Pseudoalteromonas sp.DC-2 is screened from a sea area polluted by petroleum in North China, can efficiently degrade petroleum at low temperature, especially in extreme environment of 0 DEG C. The Pseudoalteromonas sp.DC-2 can be applied in bioremediation of sea petroleum pollution, especially in petroleum spilling bioremediation at low temperature in the sea area in North China in winter (including glacial period).

The invention provides essence capable of resisting allergy, repairing skin and improving skin immunity. The essence is prepared from a water-phase ingredient and an additive phase ingredient, whereinthe water-phase ingredient is the balance of water; the additive phase ingredient is prepared from 3% to 9% of caulis dendrobii nobile lindl extractive, 5% to 9% of vegetable amino acid, 3% to 10% ofhoney extractive, 4% to 6% of pseudoalteromonas sp fermentation product extractive, 1% to 3% of honey extractive, 3% to 9% of burdock root extractive, 3% to 9% of acetyl hexapeptide-8, 3% to 8% of acetyl octapeptide, 2% to 5% of dipeptide-2, 5% to 10% of brown seaweed extractive and 6% to 9% of lactobacillus / rice fermentation product. The essence capable of resisting allergy, repairing skin and improving skin immunity is suitable for varieties of skin and can be applied to prevention of skin inflammation. A contraction effect on collagen fiber shows that the essence can astringe skin and shrink pores. Meanwhile, the essence has extremely-strong effects of replenishing water, moisturizing, nourishing skin, resisting wrinkle and quickly repairing skin and further has high safety. A preparation method of the essence capable of resisting allergy, repairing skin and improving skin immunity disclosed by the invention is simple.