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Method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam

A technology of polyurethane foam and algaecide, which is applied in directions such as being fixed on/in an organic carrier, can solve the problems of improper selection of immobilization time, low application value of free marine bacteria, and poor immobilization effect of bacteria, etc. Achieve good economic benefits, convenient and cheap mass transfer and energy transfer, and good mass and energy transfer.

Inactive Publication Date: 2011-03-23
XIAMEN UNIV
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  • Claims
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Problems solved by technology

[0012] The purpose of the present invention is to aim at the defect that the immobilization effect of bacteria is not good due to the improper selection of immobilization material or polyurethane foam density, size and immobilization time in the existing microbial adsorption process, and the practical application value of free marine bacteria It is not a high problem to provide a polyurethane foam immobilization that can not only effectively solve the shortcomings of immobilized cells by embedding, but also use polyurethane foam as an adsorption carrier, which can effectively and permanently make the marine algicide Pseudoalteromonas sp.SP48 exhibit high algicidal activity The method of marine algicide SP48

Method used

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  • Method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam
  • Method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam
  • Method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam

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Embodiment Construction

[0028] 1. Preparation of marine algicide Pseudoalteromonas sp.SP48 bacterial liquid

[0029] Put the activated SP48 bacterial solution into a 250mL Erlenmeyer flask containing 100mL Zobell 2216E medium according to the inoculation amount of 1%, and culture it at 150r / min at 28°C for 16-24h.

[0030] 2. Preparation of Polyurethane Foam

[0031] Purchase commercially available polyurethane foams of different densities, cut them into square foams of a certain size as required, and then boil the foams in boiling water for 3 to 4 times, and bake them in an oven at 70°C to constant weight.

[0032] 3. Preparation of Polyurethane Immobilized Algicide

[0033] The spare polyurethane foam was sterilized together with the algicidal culture medium, and then inserted into the activated SP48 bacterial solution at a rate of 1%, and cultured in shake flasks.

[0034] 4. Detection of algicidal activity of immobilized algicidal bacteria

[0035] Take out the immobilized polyurethane foam an...

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Abstract

The invention discloses a method for immobilizing pseudoalteromonas sp. SP48 by polyurethane foam, and relates to a microorganism adsorption and immobilization method. The invention provides a method for immobilizing the pseudoalteromonas sp. SP48 by the polyurethane foam, which can effectively overcome the defect of immobilizing cells by an entrapment method, and makes the pseudoalteromonas sp. SP48 show high-efficiency alga killing activity effectively and durably by taking the polyurethane foam as an adsorption carrier. Pseudoalteromonas sp. SP48-containing polyurethane foam is formed by sterilizing granulated, boiled and dried polyurethane foam and an alga killing bacterium culture medium together, and inoculating the SP48 for shaking culture to ensure that the SP48 enters or attaches to the inner wall of the polyurethane foam for growth. The SP48 is adsorbed and immobilized by the polyurethane foam which has a certain density and size, high adsorption capacity, no toxic or side pollution, good mass transfer and energy transfer effects, convenience and low cost; application performance in actual red tide treatment is improved remarkably; and the used polyurethane foam can be recycled through treatment, which achieves good economic benefit.

Description

technical field [0001] The invention relates to a method for adsorbing and immobilizing microorganisms, in particular to a method for adsorbing and immobilizing marine algicide Pseudoalteromonas sp. SP48 using polyurethane foam (polyurethane foam, PUF) as a carrier. Background technique [0002] Microbial adsorption and immobilization technology is a relatively new technology in the field of marine environmental protection. It is based on the static electricity, surface tension and adhesion between the charged microbial cells and the carrier, so that the microbial cells are fixed on the surface and inside of the carrier to form biological membrane method. Compared with ordinary embedding technology, adsorption immobilization technology has the following advantages: it can reduce cell growth hysteresis caused by oxygen transfer and nutrient transfer, and it will not be limited by expansion culture like embedding method, which is convenient for industrialization in production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 郑天凌周月霞田蕴吕静琳周艳艳林婧张帮周苏建强杨小茹章军
Owner XIAMEN UNIV
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