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Pseudoalteromonas with low-temperature polyaromatic hydrocarbon degradation function and application of pseudoalteromonas

A technology of alternating pseudomonas and polycyclic aromatic hydrocarbons, applied in the field of microorganisms, can solve problems such as lack of research results, and achieve the effect of simple culture method and strong naphthalene degradation ability

Active Publication Date: 2016-08-31
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few studies on low-temperature degradation bacteria of marine petroleum hydrocarbons in my country, especially the research results on low-temperature degradation bacteria of marine PAHs are even scarcer.

Method used

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  • Pseudoalteromonas with low-temperature polyaromatic hydrocarbon degradation function and application of pseudoalteromonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of bacterial strain Pseudoalteromonas sp.QN-1

[0020] Isolation of Strain Pseudoalteromonas sp.QN-1

[0021] (1) Sample collection: Jiaozhou Bay;

[0022] (2) Enrichment and acclimatization: Add 100 mL of MMC culture solution into a 250 mL Erlenmeyer flask, and sterilize at 120° C. for 15 min. Dichloromethane was sterilized by filtration with a 0.22 μm pore size organic solvent-resistant filter membrane. Weigh 0.2g of naphthalene and dissolve it in 20mL of sterilized dichloromethane, then transfer it to 100mL of sterilized MMC culture medium, and shake it on a shaker overnight to volatilize the dichloromethane. After dichloromethane volatilizes, add 5 mL of seawater sample, place it in a low-temperature biochemical incubator, shake and cultivate at 120 r / min, 10 ° C for 30 days, and then take 5 mL of culture solution from it and transfer again: add 5 mL of culture solution to the solution containing 0.2 g Naphthalene in 100mL M...

Embodiment 2

[0027] Fermentation of Example 2 bacterial strain Pseudoalteromonas sp.QN-1

[0028] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100mL of sterilized 2216E liquid medium, and culture it under low temperature shaking for 7 days (0°C, 120r / min) to become a seed solution; The liquid was added into the 2216E liquid medium at a volume ratio of 10%, and cultured at 0° C. for 7 days to obtain a fermentation liquid.

Embodiment 3

[0029] Example 3 Determination of Naphthalene Degradation Rate of Bacterial Strain Pseudoalteromonas sp.QN-1 at 0°C

[0030] (1) Degrading bacteria seed solution

[0031] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100 mL of sterilized 2216E liquid medium, and culture it with shaking at low temperature for 7 days (0°C, 120r / min).

[0032] (2) Degradation culture

[0033] Weigh 0.2g of naphthalene and dissolve it in 20mL of filter-sterilized n-hexane, transfer it to a conical flask filled with 100mL of sterilized MMC liquid medium, place it on a shaker and vibrate until the n-hexane is completely volatilized, according to 10% of the inoculation amount was used to inoculate the seed solution of the degrading bacteria, and the culture was shaken at low temperature for 30 days (0° C., 120 r / min). The culture medium not inoculated with degrading bacteria was used as the blank group.

[0034] (3) Extraction

[0035]After...

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Abstract

The invention discloses pseudoalteromonas sp.QN-1 with a low-temperature petroleum degradation function, and the collection number is CGMCC No. 11635. The pseudoalteromonas is selected from petroleum-polluted seas in north China, can degrade petroleum at a low temperature, and particularly, can degrade naphthalene in the petroleum at the extreme temperature of 0 DEG C. The pseudoalteromonas QN-1 can be applied to biological remediation of ocean petroleum pollution, and particularly, can be applied to naphthalene degradation in low-temperature environments, such as in winter (including glacial periods), at seas in north China.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a marine low-temperature bacterium and its application in bioremediation of marine naphthalene pollution. The strain can degrade petroleum hydrocarbons at a low temperature of 0°C to 15°C, especially has the characteristics of efficiently degrading naphthalene in petroleum hydrocarbons under extreme conditions of 0°C, and can be used for biological treatment of oil spill pollution and naphthalene pollution in northern seas of my country. repair work. Background technique [0002] With the rapid development of the marine transportation industry and the rapid rise of the marine mining industry, marine oil spill accidents have become increasingly frequent and harmful. In particular, oil spills in low-temperature seas cause organic pollutants to persist due to the low temperature, which will cause greater harm to the local ecosystem. Polycyclic aromatic hydrocarbons (PAHs) in petrole...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32C02F103/08
CPCC02F3/344C02F2101/327C02F2103/08C12N1/205C12R2001/01
Inventor 郭平林建国曹宾霞
Owner DALIAN MARITIME UNIVERSITY
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