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Pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and application thereof

A technology of pseudoalteromonas and bacteria, applied in the direction of bacteria, water pollutants, chemical instruments and methods, etc., can solve the problems of wastewater treatment that are rarely reported, achieve broad industrial application prospects, high removal rate, and low cost Effect

Active Publication Date: 2014-04-23
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, heavy metal microbial remediation mainly focuses on soil remediation, and there are few reports on the treatment of wastewater with heavy metals exceeding the standard.

Method used

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  • Pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and application thereof
  • Pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and application thereof
  • Pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Screening for Pseudoalteromonas sp. DSBS:

[0035] (1) 193 bacterial strains obtained from deep sea mud in the South China Sea were activated in seawater LB liquid medium and spread on solid LB medium plates containing cadmium concentrations of (50-250) mg / L respectively , place the plate upside down in the incubator and culture until colonies grow.

[0036] (2) After activating all strains with cadmium resistance level of 100mg / L for 24 hours, inoculate 2% (v / v) inoculum into 100ml seawater LB liquid medium containing cadmium concentration of 50mg / L, 200r / After 7 days of incubation on a shaker, the bacterial liquid was centrifuged at 10,000 r / min, and the remaining cadmium concentration in the supernatant was determined.

[0037] (3) Select the strain with the best cadmium removal effect.

[0038] The obtained strain can grow under the culture conditions of 15-30°C, pH value 7-8, and salinity 2%-5%. In pairs or short chains, with capsules and flagella. The colony ...

Embodiment 2

[0041] The cultivation method of Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS, the steps are as follows:

[0042] (1) Inoculate the DSBS of Pseudoalteromonas sp. obtained in Example 1 into liquid LB medium, activate and culture at 25°C for 24 hours, and prepare the activated bacterial liquid;

[0043] (2) Inoculate the activated bacterial solution prepared in step (1) into simulated wastewater at a volume fraction of 10%, shake and culture at 25°C and 200r / min for 24h, and centrifuge at 10000r / min for 10min. That is, Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS cells were obtained.

[0044] The composition of simulated wastewater in the step (2) is as follows:

[0045] Glucose 4.6g / L, sodium acetate 1.5g / L, sodium chloride 3.5g / L, magnesium sulfate heptahydrate 2.4g / L, ammonium chloride 2.6g / L, cadmium (Cd(HNO 3)2.4H 2 O) and total phosphorus (K 2 HPO 4 ) concentrations were 6.5mg / L and 7.5mg / L, and the initial pH was 7.5.

[0046] Described liquid LB culture...

Embodiment 3

[0049] The Pseudoalteromonas sp. (Pseudoalteromonas sp.) DSBS thalline that embodiment 2 is made is inoculated in the Erlenmeyer flask that 100ml simulated waste water is housed in 250ml with the inoculum of volume percentage 10%, and wherein cadmium, total phosphorus concentration are respectively 6.5mg / L and 7.5mg / L, the pH value is 7.5, cultured at 25°C for 48 hours, centrifuged to collect the supernatant, and determine the remaining cadmium ion concentration and total phosphorus content in the supernatant.

[0050] As detected by molybdenum antimony anti-spectrophotometry and flame atomic absorption method, the content of phosphorus and cadmium decreased significantly, and phosphorus was removed first, and finally the removal rates of phosphorus and cadmium were greater than 90%. The results are as follows figure 1 shown.

[0051] The cadmium-adsorbed bacterial adsorbent was centrifuged at 10,000 r / min for 10 min, and washed twice with deionized water to obtain the cadmium...

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Abstract

The invention relates to pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and an application thereof. Pseudoalteromonas sp. DSBS is collected in the China Center for Type Culture Collection (CCTCC) in Luojiashan, Wuchang, Wuhan City on December 12, 2013, with collection number of CCTCC M2013652. The strain has cadmium resistance and can still grow in cadmium-containing wastewater, and the live thallus can take full advantage of the characteristics of heavy metal adsorption and absorption, simultaneously absorbs phosphorus or forms Cd-P precipitates around the thallus in the growth process and synchronously removes cadmium and phosphorus. The toxicity of cadmium towards the strain is reduced in the presence of phosphorus.

Description

technical field [0001] The invention relates to a strain of pseudoalteromonas for efficiently removing cadmium and phosphorus in waste water and an application thereof, belonging to the technical field of waste water biological treatment. Background technique [0002] Electroplating and chemical wastewater often contain a certain concentration of cadmium and phosphorus. This type of wastewater will cause serious pollution to the environment, affect the growth of aquatic organisms, and even endanger human health. Therefore, this type of wastewater needs to be treated before it can be discharged. [0003] At present, the methods for treating heavy metal-containing wastewater are mainly physical and chemical methods such as chemical precipitation, ion exchange, and reverse osmosis. The chemical precipitation method is widely used because of its low cost and relatively mature technology. The chemical precipitation method adds chemicals (NaOH, lime, etc.) to the wastewater to ma...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20C02F101/10
Inventor 周维芝黄兆松张海鸥刘东升
Owner SHANDONG UNIV
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