Scophthalmas maximus fin rot disease antagonistic bacterium and application thereof
A technology of turbot and antagonistic bacteria, applied in the field of turbot antagonistic bacteria, can solve problems such as decline in control effect, environmental pollution, drug residues, etc., and achieve the effects of curbing occurrence and prevalence, high antagonistic activity, and strong antagonistic activity
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[0009] The present invention will be described in detail below. The screening method for the above-mentioned antagonistic bacteria is as follows: firstly, the pathogenic bacteria Vibrio anguillarum (Vibrio anguillarum), which causes hemorrhage in the fins of turbot, is used as the indicator bacteria, and more than 100 strains of bacteria isolated from Antarctic ice water and turbot culture pond water are used to screen the antagonistic bacteria. bacteria. Extraction of the extracellular product of antagonistic bacteria for the prevention of epidemic diseases of hemorrhage and death in turbot fins. The specific technical solutions are as follows:
[0010] (1) Screening of antagonistic bacteria: Inoculate the indicator bacteria, Vibrio anguillarum, a pathogen of hemorrhagic turbot fins, in LB liquid medium containing 3% NaCl, and culture with shaking at 28°C for 12 to 16 hours. Take 40 μl ~ 50 μl of indicator bacteria and spread them evenly on the high-salt nutrient agar mediu...
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