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Scophthalmas maximus fin rot disease antagonistic bacterium and application thereof

A technology of turbot and antagonistic bacteria, applied in the field of turbot antagonistic bacteria, can solve problems such as decline in control effect, environmental pollution, drug residues, etc., and achieve the effects of curbing occurrence and prevalence, high antagonistic activity, and strong antagonistic activity

Inactive Publication Date: 2013-11-13
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Since 2009, there has been an outbreak of epidemic diseases in turbot in Weihai breeding area in Shandong Province. The first symptoms are that the side fins and tail fins become lighter in color, and bleeding spots appear. Lesions, diseased turbot will die without festering. The disease mostly occurs in the seedling stage and early growing stage. It is highly contagious, widespread and has a high mortality rate. It only takes 3 to 4 days for fins to die from bleeding spots, which is an acute death and causes great economic losses to the breeding owners.
Traditional antibiotics and other chemical drug prevention and control are likely to cause bacterial resistance, resulting in a decline in the control effect year by year. At the same time, the use of chemical drugs is likely to cause drug residues, pollute the environment, and ultimately affect human health.

Method used

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Embodiment Construction

[0009] The present invention will be described in detail below. The screening method for the above-mentioned antagonistic bacteria is as follows: firstly, the pathogenic bacteria Vibrio anguillarum (Vibrio anguillarum), which causes hemorrhage in the fins of turbot, is used as the indicator bacteria, and more than 100 strains of bacteria isolated from Antarctic ice water and turbot culture pond water are used to screen the antagonistic bacteria. bacteria. Extraction of the extracellular product of antagonistic bacteria for the prevention of epidemic diseases of hemorrhage and death in turbot fins. The specific technical solutions are as follows:

[0010] (1) Screening of antagonistic bacteria: Inoculate the indicator bacteria, Vibrio anguillarum, a pathogen of hemorrhagic turbot fins, in LB liquid medium containing 3% NaCl, and culture with shaking at 28°C for 12 to 16 hours. Take 40 μl ~ 50 μl of indicator bacteria and spread them evenly on the high-salt nutrient agar mediu...

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Abstract

The invention aims to provide a scophthalmas maximus antagonistic bacterium, which is pseudoalteromonas sp. is preserved in the China General Microbiological Culture Collection Center of the Institute of Microbiology of Chinese Academy of Sciences in No. 1 Beichen West Road, Chaoyang District, Beijing on July 3rd, 2013 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7870. A bacterium which is screened by the invention with fin bleeding and deadly diseases of scophthalmas maximus is strong in antagonistic activity, and the antagonistic bacterium extracellular products prepared by utilizing a salting out method are stable in yield, low in cost and high in antagonistic activity. By using the antagonistic bacterium or antagonistic products, the immune protective rate for scophthalmas maximus is 50-60 percent; the antagonistic product is a pure biological preparation, has the characteristics of being safe and environmentally-friendly, is prevented from medicinal residues and environmental pollution; the method can be used for inhibiting occurrence and popularization of fin bleeding deadly epidemic diseases of scophthalmas maximus.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms and biological control, in particular to a turbot antagonist and application thereof. Background technique [0002] Turbot (Scophthalmas maximus) is a precious marine fish species in Europe and one of the most important marine aquaculture fish in Europe. It was introduced to my country for artificial breeding in 1992. Aquaculture species and the main object of factory farming, with an annual output value of more than 1.5 billion yuan. However, with the expansion of the breeding range, the increase of the breeding density, and the degeneration of the species, the disease problem of turbot has become increasingly prominent. Various bacterial diseases, parasitic diseases, and viral diseases have occurred from time to time, and they have become more and more serious. . Among them, bacterial disease is one of the most serious diseases in turbot farming. Since 2009, an epidemic of turbot...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/01
Inventor 郑风荣林学政刘洪展王波张白羽
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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