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Cypermethrin- and/or deltamethrin- degrading bacterium in seawater environment, and separation, purification and applications thereof

A technology of cypermethrin and deltamethrin, which is applied to cypermethrin and/or deltamethrin degrading bacteria and their separation, purification and application fields, and can solve problems such as fishery production and ecological environment impact, environment, food safety and human health hazards. , to achieve the effect of eliminating biological toxicity, broad development prospects and good degradation effect

Active Publication Date: 2014-07-09
湖州优研知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residual cypermethrin and deltamethrin can enter the water body through surface runoff or use in aquaculture sea areas, which will bring great harm to the environment, food safety and human health, and have an impact on fishery production and the ecological environment

Method used

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  • Cypermethrin- and/or deltamethrin- degrading bacterium in seawater environment, and separation, purification and applications thereof
  • Cypermethrin- and/or deltamethrin- degrading bacterium in seawater environment, and separation, purification and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0024] Screening and Identification of Degrading Strains

[0025] 1. Media and reagents

[0026] Nutrient medium: Accurately weigh 0.01 g of ferric phosphate, 5.0 g of peptone, and 1.0 g of yeast extract, add 1,000 mL of filtered seawater, mix well, adjust the pH to 7.0-7.8, and prepare after high-pressure steam sterilization (121°C, 20 min). have to.

[0027] Enrichment culture solution: Add cypermethrin and deltamethrin solutions to the nutrient medium so that the concentrations of both cypermethrin and deltamethrin are 100 mg / L.

[0028] 2. Isolation and purification of bacterial strains

[0029] The seawater samples were collected from the coastal waters of Ningbo, and 500 mL of seawater was collected and enriched by filtration in a sterile state, and then added to the enrichment culture solution containing 100 mg / L of cypermethrin and deltamethrin, and heated at 28 °C and 180 r After 7 days of culture on a shaker / min shaker, transfer 10% of the inoculum to the above-me...

specific Embodiment 2

[0031] Identification of strains degrading lambda-cypermethrin and / or deltamethrin pesticides

[0032] The bacterial strain obtained above is carried out morphological characteristic and molecular biological identification, and the Gram staining microscope picture of this bacterial strain is as follows: figure 1 As shown, the scanning electron microscope image is shown in figure 2 As shown, the main biological characteristics of the strain are: Gram stain negative, bacillus, arginine double hydrolysis test, lysine decarboxylase test, citrate test, ornithine decarboxylation test, urease test positive , V-P reaction, H 2 S reaction, gelatin liquefaction test negative, can use sucrose. The strain grows in seawater, the optimum growth temperature is 25-30°C, and the optimum growth pH is 7.0-7.8. On solid medium, the colonies are round, raised, with complete edges, off-white, and translucent.

[0033] The serial number of the strain is JX087926. The BLAST homology comparison...

specific Embodiment 3

[0034] Degradation effect and application of cypermethrin and / or deltamethrin degrading bacteria on pyrethroid pesticides in seawater

[0035]Inoculate the isolated and purified strains into 100 mL of enrichment medium containing 100 mg / L of cypermethrin and deltamethrin (the preparation method is as shown in Example 1), and use the enrichment medium without inoculation as a control , and cultured on a constant temperature shaker at 28°C and 180 rpm for 7 days. After culturing, draw 2 mL of culture solution, add 0.4 g NacL, vortex and mix well, and use 4 mL of a mixed solution of n-hexane and acetone (volume ratio 1:1) and 2 mL of a mixed solution of n-hexane and acetone Extract, combine the supernatant obtained from two extractions, take 3 mL of the supernatant, add 0.4 g of anhydrous sodium sulfate, dry it with nitrogen, and dissolve it with a mixed solution of 1 mL of isooctane and acetone (volume ratio 1:1), For GC-ECD analysis and detection, each experiment was repeated ...

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Abstract

The invention discloses a cypermethrin- and / or deltamethrin- degrading bacterium in seawater environment, and separation, purification and applications thereof. The bacterium, and the separation, the purification and the applications thereof are characterized in that: the bacterium is an HS-10 bacterial strain and is classified and named as Pseudoalteromonas sp.; the bacterium is preserved in the common microbe center of China Committee for Culture Collection of Microorganisms on 24 April 2012; the accession number of the bacterium is CGMCCNo.6044; the Genbank accession number of the 16SrDNA of the bacterium is JX087926; and the bacterium is advantageous in that the bacterium can safely, efficiently and rapidly degrade cypermethrin and deltamethrin in the seawater environment, and the bacterium has a good application prospect in the fields of offshore environment protection, treatment of pesticide residue pollution in a breeding environment and food safety guaranteeing.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a cypermethrin and / or deltamethrin degrading bacteria in a seawater environment and its separation, purification and application. Background technique [0002] Organophosphorus pesticides have the characteristics of high efficiency, low residue and low use cost. They are currently the most widely used agricultural chemicals with many varieties and high yields. The use of organophosphorus pesticides has produced huge economic and social benefits in terms of agricultural production increase and disease and pest vector control, but pyrethroids have been used as organochlorine pesticides due to their high efficiency, broad spectrum, and relatively low toxicity to non-target organisms. And organophosphorus pesticide substitutes are widely used in agricultural production, and are currently widely used in orchards, tea gardens, farmland and fish farming and other pest control. Howe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/04A62D101/28C02F101/38
Inventor 史西志张蓉蓉孙爱丽刘菁华李德祥陈炯
Owner 湖州优研知识产权服务有限公司
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