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Enterobacter cloacae and application of enterobacter cloacae

A technology for the promotion of Enterobacter cloacae and plant growth, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of reduced utilization rate, damage to the soil environment, limited yield of leeks, etc., and achieve the effect of avoiding discomfort

Active Publication Date: 2012-11-21
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Planting leeks requires a large amount of chemical fertilizers, which not only affects the quality of leeks, but also damages the soil environment. It is a well-known fact that with the extensive use of chemical fertilizers, their utilization rate continues to decrease
This shows that it is limited to increase the yield of leek by increasing the application of chemical fertilizers in large quantities.

Method used

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  • Enterobacter cloacae and application of enterobacter cloacae
  • Enterobacter cloacae and application of enterobacter cloacae
  • Enterobacter cloacae and application of enterobacter cloacae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1, Isolation and Identification of Enterobacter cloacae SXH-1

[0042] 1. Obtaining of strain SXH-1

[0043] 1. Sampling

[0044] The leeks and soil for the test were taken from Shuangjing Town, Hulan District, Harbin City, Heilongjiang Province (soil physical and chemical properties: pH8.15, total salt content 1.83%, alkaline hydrolyzed nitrogen 36mg / kg, available phosphorus 8mg / kg, available phosphorus 132mg / kg, organic matter 2.69%). Put leeks and plant rhizosphere soil into clean fresh-keeping bags prepared in advance, and bring them back to the laboratory for planting for testing.

[0045] 2. Screening and purification

[0046] (1) Take 1g of rhizosphere soil sample in 50mL of PAF medium, shake culture at 28°C. PAF medium contains peptone, casein hydrolyzate, MgSO 4 、K 2 HPO 4 and glycerin.

[0047] (2) Take 1mL of the shaken PAF culture solution from (1), put it in 50mL of PAF medium, and culture it with shaking at 28°C.

[0048] (3) Take 1 mL of ...

Embodiment 2

[0066] Example 2, the ability of Enterobacter cloacae SXH-1 to produce ACC deaminase

[0067] Experimental mechanism: 1-aminocyclopropane-1-carboxylic acid is degraded under the action of ACC deaminase to generate α-butyruvate (α-KA) and ammonia gas.

[0068] The experimental steps are as follows:

[0069] 1. Cultivate Enterobacter cloacae SXH-1 overnight in TSB culture medium, collect the bacteria by centrifugation at 4°C and wash three times with DF culture medium.

[0070] 2. Suspend the bacteria obtained in step 1 in ADF culture medium, shake and culture at room temperature (21±1°C) for 2 days, collect the bacteria by centrifugation at 4°C, and wash three times with 0.1mol / L Tris-HCl buffer (pH=7.6) .

[0071] 3. Resuspend the bacteria obtained in step 2 (wet weight about 0.005g) in 600μL 0.1mol / L Tris-HCl buffer (pH=8.0), add 30μL toluene and shake rapidly for 30s (using a vortex shaker) To break the cells, the whole broken system is the cell extract.

[0072] 4. Grou...

Embodiment 3

[0079] Example 3, the ability of Enterobacter cloacae SXH-1 to produce growth hormone (indole acetic acid, IAA)

[0080]l. Incubate Enterobacter cloacae SXH-1 in DF culture medium at 28°C and 180 r min -1 Shake culture for 2 days, and then micro-transfer into DF culture medium supplemented with different concentrations of tryptophan (L-Trp) (the concentration of L-Trp is 0, 50, 100, 200 or 500 μg·mL -1 ) for 2 days, and samples were taken at 600nm to measure the absorbance (OD 600nm Numerical value), and the rest of the culture medium was centrifuged at 8000g at room temperature to obtain the supernatant.

[0081] 2. Take 500 μL of the supernatant from step 1, add 2 mL of Salkowski reagent (150 mL of H 2 SO 4 , 250mLddH 2 O and 7.5mL 0.5mol / L FeCl 3 . Aqueous solution, the rest is water), and measure the absorbance (OD 535nm value).

[0082] The DF culture medium was used instead of the supernatant in step 1 to carry out the experiment in step 2, as a zero-setting proc...

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Abstract

The invention discloses enterobacter cloacae and application of the enterobacter cloacae. The enterobacter cloacae SXH-1 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6296. The invention also protects the application of the enterobacter cloacae SXH-1 to the plant growth promotion. The plant is concretely chives. The enterobacter cloacae SXH-1 provided by the invention can use ACC (aminocyclopropane carboxylic acid) as the unique nitrogen source for growth, and meanwhile, the ACC is decomposed. The strain is hopeful to realize important effects in the chive growth promotion aspect.

Description

technical field [0001] The invention relates to a strain of Enterobacter cloacae and its application. Background technique [0002] Leek (Allium tuberosum Rottl. ex Spr.) is a perennial perennial plant of the genus Allium tuberosum in the family Liliaceae. It is native to China and is cultivated all over the country. [0003] Leek is rich in vitamins and various minerals, and contains a variety of sulfides and has a special aroma, which can increase appetite and has certain medicinal value. Its seeds and leaves can be used as medicine, which has the effects of invigorating the stomach, refreshing, antiperspirant and astringent, tonifying kidney and yang, and solidifying essence. In traditional Chinese medicine, leeks have a very famous name called "Yangyang grass", and some people call leeks "intestinal cleansing grass". Wait. Because of its good taste and delicious taste, leek is loved by everyone, especially before the Spring Festival, so it is an economical plant. [...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/88A01N63/02A01P21/00C12P17/10C12P1/04C12R1/01
Inventor 郭长虹史煦涵马军蔡洪生霍智慧
Owner HARBIN NORMAL UNIVERSITY
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